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Cytokinin-induced gene expression in cultured green cells of Nicotiana tabacum identified by fluorescent differential display

The cell growth and plastid development of cultured green tobacco cells were maintained by the phytohormone cytokinin. After subculture into cytokinin-free medium, when cytokinin treatment was resumed, physiological changes, induced by cytokinin were analyzed. Changes in chlorophyll biosynthesis and... Full description

Journal Title: Bioscience Biotechnology, and Biochemistry, 2001, Vol.(6), pp.1275-1283
Main Author: Kimura , T.
Other Authors: Nakano , T. , Taki , N. , Ishikawa , M. , Asami , T. , Yoshida , S.
Format: Electronic Article Electronic Article
Language: English
Subjects:
Quelle: AGRIS (Food and Agriculture Organization of the United Nations)
ID: ISSN: 0916-8451
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recordid: faoagrisJP2002000926
title: Cytokinin-induced gene expression in cultured green cells of Nicotiana tabacum identified by fluorescent differential display
format: Article
creator:
  • Kimura , T.
  • Nakano , T.
  • Taki , N.
  • Ishikawa , M.
  • Asami , T.
  • Yoshida , S.
subjects:
  • Nicotiana Tabacum
  • Cytokinins
  • Gene Expression
  • Methods
ispartof: Bioscience, Biotechnology, and Biochemistry, 2001, Vol.(6), pp.1275-1283
description: The cell growth and plastid development of cultured green tobacco cells were maintained by the phytohormone cytokinin. After subculture into cytokinin-free medium, when cytokinin treatment was resumed, physiological changes, induced by cytokinin were analyzed. Changes in chlorophyll biosynthesis and photosynthetic gene expression were observed 1 week after cytokinin induction, and changes in cell growth were observed 2 weeks after cytokinin induction. Two cytokinin-induced genes (cig) were isolated from these cells using the fluorescent differential display technique. Northern analysis confirmed that expression of these cig was induced by both natural and synthetic cytokinins. The expression of cig1 was also induced by abscisic acid, and its cDNA sequence was similar to the proline dehydrogenase gene. The expression of cig2 is specific to cytokinin and is not induced by other phytohormones. The amino acid sequence encoded by cig2 is similar to the GDP / GTP exchange factor eIF2B, which regulates translation initiation. The expression of these cig suggests a complex induction system involving cytokinin and other phytohormones. ; Summary (En) ; 7 fig.; 38 ref.
language: eng
source: AGRIS (Food and Agriculture Organization of the United Nations)
identifier: ISSN: 0916-8451
fulltext: fulltext
issn:
  • 09168451
  • 0916-8451
url: Link


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titleCytokinin-induced gene expression in cultured green cells of Nicotiana tabacum identified by fluorescent differential display
creatorKimura , T. ; Nakano , T. ; Taki , N. ; Ishikawa , M. ; Asami , T. ; Yoshida , S.
ispartofBioscience, Biotechnology, and Biochemistry, 2001, Vol.(6), pp.1275-1283
identifierISSN: 0916-8451
subjectNicotiana Tabacum ; Cytokinins ; Gene Expression ; Methods
descriptionThe cell growth and plastid development of cultured green tobacco cells were maintained by the phytohormone cytokinin. After subculture into cytokinin-free medium, when cytokinin treatment was resumed, physiological changes, induced by cytokinin were analyzed. Changes in chlorophyll biosynthesis and photosynthetic gene expression were observed 1 week after cytokinin induction, and changes in cell growth were observed 2 weeks after cytokinin induction. Two cytokinin-induced genes (cig) were isolated from these cells using the fluorescent differential display technique. Northern analysis confirmed that expression of these cig was induced by both natural and synthetic cytokinins. The expression of cig1 was also induced by abscisic acid, and its cDNA sequence was similar to the proline dehydrogenase gene. The expression of cig2 is specific to cytokinin and is not induced by other phytohormones. The amino acid sequence encoded by cig2 is similar to the GDP / GTP exchange factor eIF2B, which regulates translation initiation. The expression of these cig suggests a complex induction system involving cytokinin and other phytohormones. ; Summary (En) ; 7 fig.; 38 ref.
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descriptionThe cell growth and plastid development of cultured green tobacco cells were maintained by the phytohormone cytokinin. After subculture into cytokinin-free medium, when cytokinin treatment was resumed, physiological changes, induced by cytokinin were analyzed. Changes in chlorophyll biosynthesis and photosynthetic gene expression were observed 1 week after cytokinin induction, and changes in cell growth were observed 2 weeks after cytokinin induction. Two cytokinin-induced genes (cig) were isolated from these cells using the fluorescent differential display technique. Northern analysis confirmed that expression of these cig was induced by both natural and synthetic cytokinins. The expression of cig1 was also induced by abscisic acid, and its cDNA sequence was similar to the proline dehydrogenase gene. The expression of cig2 is specific to cytokinin and is not induced by other phytohormones. The amino acid sequence encoded by cig2 is similar to the GDP / GTP exchange factor eIF2B, which regulates translation initiation. The expression of these cig suggests a complex induction system involving cytokinin and other phytohormones. ; Summary (En) ; 7 fig.; 38 ref.
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titleCytokinin-induced gene expression in cultured green cells of Nicotiana tabacum identified by fluorescent differential display
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abstractThe cell growth and plastid development of cultured green tobacco cells were maintained by the phytohormone cytokinin. After subculture into cytokinin-free medium, when cytokinin treatment was resumed, physiological changes, induced by cytokinin were analyzed. Changes in chlorophyll biosynthesis and photosynthetic gene expression were observed 1 week after cytokinin induction, and changes in cell growth were observed 2 weeks after cytokinin induction. Two cytokinin-induced genes (cig) were isolated from these cells using the fluorescent differential display technique. Northern analysis confirmed that expression of these cig was induced by both natural and synthetic cytokinins. The expression of cig1 was also induced by abscisic acid, and its cDNA sequence was similar to the proline dehydrogenase gene. The expression of cig2 is specific to cytokinin and is not induced by other phytohormones. The amino acid sequence encoded by cig2 is similar to the GDP / GTP exchange factor eIF2B, which regulates translation initiation. The expression of these cig suggests a complex induction system involving cytokinin and other phytohormones.
date2001-06