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Molecular characterization of photomixotrophic tobacco cells resistant to protoporphyrinogen oxidase-inhibiting herbicides

Peroxidizing herbicides inhibit protoporphyrinogen oxidase (Protox), the last enzyme of the common branch of the chlorophyll- and heme-synthesis pathways. There are two isoenzymes of Protox, one of which is located in the plastid and the other in the mitochondria. Sequence analysis of the cloned Pro... Full description

Journal Title: Plant physiology Lancaster, Pa., 1998, Vol.(3), pp.751-758
Main Author: Watanabe , N.
Other Authors: Che , F.S. , Iwano , M. , Takayama , S. , Nakano , T. , Yoshida , S. , Isogai , A.
Format: Electronic Article Electronic Article
Language: English
Subjects:
Quelle: AGRIS (Food and Agriculture Organization of the United Nations)
ID: ISSN: 0032-0889
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recordid: faoagrisUS1999008963
title: Molecular characterization of photomixotrophic tobacco cells resistant to protoporphyrinogen oxidase-inhibiting herbicides
format: Article
creator:
  • Watanabe , N.
  • Che , F.S.
  • Iwano , M.
  • Takayama , S.
  • Nakano , T.
  • Yoshida , S.
  • Isogai , A.
subjects:
  • Nicotiana Tabacum
  • Cell Culture
  • Chlorophylls
  • Haemoglobin
  • Biosynthesis
  • Oxidoreductases
  • Isoenzymes
  • Enzymic Activity
  • Enzyme Inhibitors
  • Oxidation
  • Herbicides
  • Chemical Reactions
  • Biochemical Pathways
  • Genetics
  • Messenger Rna
  • Genes
  • Nucleotide Sequence
  • Gene Expression
  • Genotypes
  • Genetic Variation
  • Plastids
  • Mitochondria
  • Cell Structure
  • Ultrastructure
  • Nicotiana Tabacum
  • Culture De Cellule
  • Chlorophylle
  • Hemoglobine
  • Biosynthese
  • Oxydoreductase
  • Isoenzyme
  • Activite Enzymatique
  • Inhibiteur D'Enzyme
  • Oxydation
  • Herbicide
  • Reaction Chimique
  • Voie Biochimique Du Metabolisme
  • Genetique
  • Arn Messager
  • Gene
  • Sequence Nucleotidique
  • Expression Des Genes
  • Genotype
  • Variation Genetique
  • Plaste
  • Mitochondrie
  • Structure Cellulaire
  • Ultrastructure
  • Nicotiana Tabacum
  • Cultivo De Celulas
  • Clorofilas
  • Hemoglobina
  • Biosintesis
  • Oxidorreductasas
  • Isoenzimas
  • Actividad Enzimatica
  • Inhibidores De Enzimas
  • Oxidacion
  • Herbicidas
  • Reacciones Quimicas
  • Via Bioquimica Del Metabolismo
  • Genetica
  • Arn Mensajero
  • Genes
  • Secuencia Nucleotidica
  • Expresion Genica
  • Genotipos
  • Variacion Genetica
  • Plastidios
  • Mitocondria
  • Estructura Celular
  • Ultraestructura
  • Molecular Sequence Data
  • Heme
  • Peroxidation
  • Genetic Regulation
  • Localization
ispartof: Plant physiology, Lancaster, Pa., 1998, Vol.(3), pp.751-758
description: Peroxidizing herbicides inhibit protoporphyrinogen oxidase (Protox), the last enzyme of the common branch of the chlorophyll- and heme-synthesis pathways. There are two isoenzymes of Protox, one of which is located in the plastid and the other in the mitochondria. Sequence analysis of the cloned Protox cDNAs showed that the deduced amino acid sequences of plastidial and mitochondrial Protox in wild-type cells and in herbicide-resistant YZI-1S cells are the same. The level of plastidial Protox mRNA was the same in both wild-type and YZI-1S cells, whereas the level of mitochondrial Protox mRNA YZI-1S cells was up to 10 times the level of wild-type cells. Wild-type cells were observed by fluorescence microscopy to emit strong autofluorescence from chlorophyll. Only a weak fluorescence signal was observed from chlorophyll in YZI-1S cells grown in the Protox inhibitor N-(4-chloro-2-fluoro-5-propagyloxy)-phenyl-3,4,5, 6-tetrahydrophthalimide. Staining with DiOC6 showed no visible difference in the number or strength of fluorescence between wild-type and YZI-1S mitochondria. Electron micrography of YZI-1S cells showed that, in contrast to wild-type cells, the chloroplasts of YZI-1S cells grown in the presence of N-(4-chloro-2-fluoro-5-propagyloxy)-phenyl- 3,4,5,6-tetrahydrophthalimide exhibited no grana stacking. These results suggest that the herbicide resistance of YZI-1S cells is due to the overproduction of mitochondrial Protox ; references
language: eng
source: AGRIS (Food and Agriculture Organization of the United Nations)
identifier: ISSN: 0032-0889
fulltext: fulltext
issn:
  • 00320889
  • 0032-0889
url: Link


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titleMolecular characterization of photomixotrophic tobacco cells resistant to protoporphyrinogen oxidase-inhibiting herbicides
creatorWatanabe , N. ; Che , F.S. ; Iwano , M. ; Takayama , S. ; Nakano , T. ; Yoshida , S. ; Isogai , A.
ispartofPlant physiology, Lancaster, Pa., 1998, Vol.(3), pp.751-758
identifierISSN: 0032-0889
subjectNicotiana Tabacum ; Cell Culture ; Chlorophylls ; Haemoglobin ; Biosynthesis ; Oxidoreductases ; Isoenzymes ; Enzymic Activity ; Enzyme Inhibitors ; Oxidation ; Herbicides ; Chemical Reactions ; Biochemical Pathways ; Genetics ; Messenger Rna ; Genes ; Nucleotide Sequence ; Gene Expression ; Genotypes ; Genetic Variation ; Plastids ; Mitochondria ; Cell Structure ; Ultrastructure ; Nicotiana Tabacum ; Culture De Cellule ; Chlorophylle ; Hemoglobine ; Biosynthese ; Oxydoreductase ; Isoenzyme ; Activite Enzymatique ; Inhibiteur D'Enzyme ; Oxydation ; Herbicide ; Reaction Chimique ; Voie Biochimique Du Metabolisme ; Genetique ; Arn Messager ; Gene ; Sequence Nucleotidique ; Expression Des Genes ; Genotype ; Variation Genetique ; Plaste ; Mitochondrie ; Structure Cellulaire ; Ultrastructure ; Nicotiana Tabacum ; Cultivo De Celulas ; Clorofilas ; Hemoglobina ; Biosintesis ; Oxidorreductasas ; Isoenzimas ; Actividad Enzimatica ; Inhibidores De Enzimas ; Oxidacion ; Herbicidas ; Reacciones Quimicas ; Via Bioquimica Del Metabolismo ; Genetica ; Arn Mensajero ; Genes ; Secuencia Nucleotidica ; Expresion Genica ; Genotipos ; Variacion Genetica ; Plastidios ; Mitocondria ; Estructura Celular ; Ultraestructura ; Molecular Sequence Data ; Heme ; Peroxidation ; Genetic Regulation ; Localization
descriptionPeroxidizing herbicides inhibit protoporphyrinogen oxidase (Protox), the last enzyme of the common branch of the chlorophyll- and heme-synthesis pathways. There are two isoenzymes of Protox, one of which is located in the plastid and the other in the mitochondria. Sequence analysis of the cloned Protox cDNAs showed that the deduced amino acid sequences of plastidial and mitochondrial Protox in wild-type cells and in herbicide-resistant YZI-1S cells are the same. The level of plastidial Protox mRNA was the same in both wild-type and YZI-1S cells, whereas the level of mitochondrial Protox mRNA YZI-1S cells was up to 10 times the level of wild-type cells. Wild-type cells were observed by fluorescence microscopy to emit strong autofluorescence from chlorophyll. Only a weak fluorescence signal was observed from chlorophyll in YZI-1S cells grown in the Protox inhibitor N-(4-chloro-2-fluoro-5-propagyloxy)-phenyl-3,4,5, 6-tetrahydrophthalimide. Staining with DiOC6 showed no visible difference in the number or strength of fluorescence between wild-type and YZI-1S mitochondria. Electron micrography of YZI-1S cells showed that, in contrast to wild-type cells, the chloroplasts of YZI-1S cells grown in the presence of N-(4-chloro-2-fluoro-5-propagyloxy)-phenyl- 3,4,5,6-tetrahydrophthalimide exhibited no grana stacking. These results suggest that the herbicide resistance of YZI-1S cells is due to the overproduction of mitochondrial Protox ; references
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1Che , F.S.
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4Nakano , T.
5Yoshida , S.
6Isogai , A.
titleMolecular characterization of photomixotrophic tobacco cells resistant to protoporphyrinogen oxidase-inhibiting herbicides
descriptionPeroxidizing herbicides inhibit protoporphyrinogen oxidase (Protox), the last enzyme of the common branch of the chlorophyll- and heme-synthesis pathways. There are two isoenzymes of Protox, one of which is located in the plastid and the other in the mitochondria. Sequence analysis of the cloned Protox cDNAs showed that the deduced amino acid sequences of plastidial and mitochondrial Protox in wild-type cells and in herbicide-resistant YZI-1S cells are the same. The level of plastidial Protox mRNA was the same in both wild-type and YZI-1S cells, whereas the level of mitochondrial Protox mRNA YZI-1S cells was up to 10 times the level of wild-type cells. Wild-type cells were observed by fluorescence microscopy to emit strong autofluorescence from chlorophyll. Only a weak fluorescence signal was observed from chlorophyll in YZI-1S cells grown in the Protox inhibitor N-(4-chloro-2-fluoro-5-propagyloxy)-phenyl-3,4,5, 6-tetrahydrophthalimide. Staining with DiOC6 showed no visible difference in the number or strength of fluorescence between wild-type and YZI-1S mitochondria. Electron micrography of YZI-1S cells showed that, in contrast to wild-type cells, the chloroplasts of YZI-1S cells grown in the presence of N-(4-chloro-2-fluoro-5-propagyloxy)-phenyl- 3,4,5,6-tetrahydrophthalimide exhibited no grana stacking. These results suggest that the herbicide resistance of YZI-1S cells is due to the overproduction of mitochondrial Protox ; references
subject
0Nicotiana Tabacum
1Cell Culture
2Chlorophylls
3Haemoglobin
4Biosynthesis
5Oxidoreductases
6Isoenzymes
7Enzymic Activity
8Enzyme Inhibitors
9Oxidation
10Herbicides
11Chemical Reactions
12Biochemical Pathways
13Genetics
14Messenger Rna
15Genes
16Nucleotide Sequence
17Gene Expression
18Genotypes
19Genetic Variation
20Plastids
21Mitochondria
22Cell Structure
23Ultrastructure
24Culture De Cellule
25Chlorophylle
26Hemoglobine
27Biosynthese
28Oxydoreductase
29Isoenzyme
30Activite Enzymatique
31Inhibiteur D'Enzyme
32Oxydation
33Herbicide
34Reaction Chimique
35Voie Biochimique Du Metabolisme
36Genetique
37Arn Messager
38Gene
39Sequence Nucleotidique
40Expression Des Genes
41Genotype
42Variation Genetique
43Plaste
44Mitochondrie
45Structure Cellulaire
46Cultivo De Celulas
47Clorofilas
48Hemoglobina
49Biosintesis
50Oxidorreductasas
51Isoenzimas
52Actividad Enzimatica
53Inhibidores De Enzimas
54Oxidacion
55Herbicidas
56Reacciones Quimicas
57Via Bioquimica Del Metabolismo
58Genetica
59Arn Mensajero
60Secuencia Nucleotidica
61Expresion Genica
62Genotipos
63Variacion Genetica
64Plastidios
65Mitocondria
66Estructura Celular
67Ultraestructura
68Molecular Sequence Data
69Heme
70Peroxidation
71Genetic Regulation
72Localization
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titleMolecular characterization of photomixotrophic tobacco cells resistant to protoporphyrinogen oxidase-inhibiting herbicides
authorWatanabe , N. ; Che , F.S. ; Iwano , M. ; Takayama , S. ; Nakano , T. ; Yoshida , S. ; Isogai , A.
creationdate19981100
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0Nicotiana Tabacum
1Cell Culture
2Chlorophylls
3Haemoglobin
4Biosynthesis
5Oxidoreductases
6Isoenzymes
7Enzymic Activity
8Enzyme Inhibitors
9Oxidation
10Herbicides
11Chemical Reactions
12Biochemical Pathways
13Genetics
14Messenger Rna
15Genes
16Nucleotide Sequence
17Gene Expression
18Genotypes
19Genetic Variation
20Plastids
21Mitochondria
22Cell Structure
23Ultrastructure
24Culture De Cellule
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26Hemoglobine
27Biosynthese
28Oxydoreductase
29Isoenzyme
30Activite Enzymatique
31Inhibiteur D'Enzyme
32Oxydation
33Herbicide
34Reaction Chimique
35Voie Biochimique Du Metabolisme
36Genetique
37Arn Messager
38Gene
39Sequence Nucleotidique
40Expression Des Genes
41Genotype
42Variation Genetique
43Plaste
44Mitochondrie
45Structure Cellulaire
46Cultivo De Celulas
47Clorofilas
48Hemoglobina
49Biosintesis
50Oxidorreductasas
51Isoenzimas
52Actividad Enzimatica
53Inhibidores De Enzimas
54Oxidacion
55Herbicidas
56Reacciones Quimicas
57Via Bioquimica Del Metabolismo
58Genetica
59Arn Mensajero
60Secuencia Nucleotidica
61Expresion Genica
62Genotipos
63Variacion Genetica
64Plastidios
65Mitocondria
66Estructura Celular
67Ultraestructura
68Molecular Sequence Data
69Heme
70Peroxidation
71Genetic Regulation
72Localization
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1Che , F.S.
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6Isogai , A.
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1Che, F.S.
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4Nakano, T.
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6Isogai, A.
atitleMolecular characterization of photomixotrophic tobacco cells resistant to protoporphyrinogen oxidase-inhibiting herbicides
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abstractPeroxidizing herbicides inhibit protoporphyrinogen oxidase (Protox), the last enzyme of the common branch of the chlorophyll- and heme-synthesis pathways. There are two isoenzymes of Protox, one of which is located in the plastid and the other in the mitochondria. Sequence analysis of the cloned Protox cDNAs showed that the deduced amino acid sequences of plastidial and mitochondrial Protox in wild-type cells and in herbicide-resistant YZI-1S cells are the same. The level of plastidial Protox mRNA was the same in both wild-type and YZI-1S cells, whereas the level of mitochondrial Protox mRNA YZI-1S cells was up to 10 times the level of wild-type cells. Wild-type cells were observed by fluorescence microscopy to emit strong autofluorescence from chlorophyll. Only a weak fluorescence signal was observed from chlorophyll in YZI-1S cells grown in the Protox inhibitor N-(4-chloro-2-fluoro-5-propagyloxy)-phenyl-3,4,5, 6-tetrahydrophthalimide. Staining with DiOC6 showed no visible difference in the number or strength of fluorescence between wild-type and YZI-1S mitochondria. Electron micrography of YZI-1S cells showed that, in contrast to wild-type cells, the chloroplasts of YZI-1S cells grown in the presence of N-(4-chloro-2-fluoro-5-propagyloxy)-phenyl- 3,4,5,6-tetrahydrophthalimide exhibited no grana stacking. These results suggest that the herbicide resistance of YZI-1S cells is due to the overproduction of mitochondrial Protox
date1998-11