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Sperm cryopreservation of yellow drum Nibea albiflora: A special emphasis on post-thaw sperm quality

This study was intended to develop an optimal sperm cryopreservation protocol for yellow drum (Nibea albiflora), a commercially important fish species in East Asia. Specifically, we evaluated cryoprotectant type, concentration, and cooling rate and found that the highest fertilization (56–61%) and h... Full description

Journal Title: Aquaculture 2012, Vol.368, pp.82-88
Main Author: Dai , Tongren
Other Authors: Zhao , Enhui , Lu , Gang , Che , Kai , He , Qiutao , Lu , Yonglin , Fang , Qiongshan , Wang , Hansheng , Zheng , Leyun , Li , Suping , Huang , Changjiang , Dong , Qiaoxiang
Format: Electronic Article Electronic Article
Language: English
Subjects:
Quelle: AGRIS (Food and Agriculture Organization of the United Nations)
ID: ISSN: 0044-8486
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recordid: faoagrisUS201400186167
title: Sperm cryopreservation of yellow drum Nibea albiflora: A special emphasis on post-thaw sperm quality
format: Article
creator:
  • Dai , Tongren
  • Zhao , Enhui
  • Lu , Gang
  • Che , Kai
  • He , Qiutao
  • Lu , Yonglin
  • Fang , Qiongshan
  • Wang , Hansheng
  • Zheng , Leyun
  • Li , Suping
  • Huang , Changjiang
  • Dong , Qiaoxiang
subjects:
  • Lipids
  • Fish
  • Membrane Potential
  • Hatching
  • Nibea Albiflora
  • Dna Fragmentation
  • Ethylene Glycol
  • Sperm Motility
  • Eggs
  • Lipid Composition
  • Cryopreservation
  • Spermatozoa
  • Dimethyl Sulfoxide
ispartof: Aquaculture, 2012, Vol.368, pp.82-88
description: This study was intended to develop an optimal sperm cryopreservation protocol for yellow drum (Nibea albiflora), a commercially important fish species in East Asia. Specifically, we evaluated cryoprotectant type, concentration, and cooling rate and found that the highest fertilization (56–61%) and hatch (42–47%) can be obtained from yellow drum sperm suspended in 5% ethylene glycol or 10% dimethyl sulfoxide, cooled at 220°C/min, thawed in 40°C water bath for 7s, and fertilized with fresh (non-frozen) eggs at a sperm-to-egg ratio of 10⁷. We also compared other sperm quality parameters such as sperm motility, motility duration, membrane integrity, mitochondrial membrane potential, and DNA fragmentation between fresh and thawed sperm samples. Although cryopreservation resulted in a significant increase in DNA fragmentation and significant decreases in the remaining parameters, the magnitude of these changes was small when compared to the 2000 to 3000-fold reduction in fertilization/hatch capability of thawed sperm. These findings suggest possible disruption of signaling pathways that cannot be detected by conventional quality assays. Further analysis of sperm membrane lipid composition revealed a significant negative correlation between sperm quality and cholesterol-to-sphingomyelin ratio. These findings indicate that lipid rafts could be the main targets for cryodamage-induced decrease in fertilization capability of thawed sperm. Future studies are necessary to further explore the role of lipid rafts in cryopreservation-mediated damage in fish sperm. ; p. 82-88.
language: eng
source: AGRIS (Food and Agriculture Organization of the United Nations)
identifier: ISSN: 0044-8486
fulltext: fulltext
issn:
  • 00448486
  • 0044-8486
url: Link


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titleSperm cryopreservation of yellow drum Nibea albiflora: A special emphasis on post-thaw sperm quality
creatorDai , Tongren ; Zhao , Enhui ; Lu , Gang ; Che , Kai ; He , Qiutao ; Lu , Yonglin ; Fang , Qiongshan ; Wang , Hansheng ; Zheng , Leyun ; Li , Suping ; Huang , Changjiang ; Dong , Qiaoxiang
ispartofAquaculture, 2012, Vol.368, pp.82-88
identifierISSN: 0044-8486
subjectLipids ; Fish ; Membrane Potential ; Hatching ; Nibea Albiflora ; Dna Fragmentation ; Ethylene Glycol ; Sperm Motility ; Eggs ; Lipid Composition ; Cryopreservation ; Spermatozoa ; Dimethyl Sulfoxide
descriptionThis study was intended to develop an optimal sperm cryopreservation protocol for yellow drum (Nibea albiflora), a commercially important fish species in East Asia. Specifically, we evaluated cryoprotectant type, concentration, and cooling rate and found that the highest fertilization (56–61%) and hatch (42–47%) can be obtained from yellow drum sperm suspended in 5% ethylene glycol or 10% dimethyl sulfoxide, cooled at 220°C/min, thawed in 40°C water bath for 7s, and fertilized with fresh (non-frozen) eggs at a sperm-to-egg ratio of 10⁷. We also compared other sperm quality parameters such as sperm motility, motility duration, membrane integrity, mitochondrial membrane potential, and DNA fragmentation between fresh and thawed sperm samples. Although cryopreservation resulted in a significant increase in DNA fragmentation and significant decreases in the remaining parameters, the magnitude of these changes was small when compared to the 2000 to 3000-fold reduction in fertilization/hatch capability of thawed sperm. These findings suggest possible disruption of signaling pathways that cannot be detected by conventional quality assays. Further analysis of sperm membrane lipid composition revealed a significant negative correlation between sperm quality and cholesterol-to-sphingomyelin ratio. These findings indicate that lipid rafts could be the main targets for cryodamage-induced decrease in fertilization capability of thawed sperm. Future studies are necessary to further explore the role of lipid rafts in cryopreservation-mediated damage in fish sperm. ; p. 82-88.
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titleSperm cryopreservation of yellow drum Nibea albiflora: A special emphasis on post-thaw sperm quality
descriptionThis study was intended to develop an optimal sperm cryopreservation protocol for yellow drum (Nibea albiflora), a commercially important fish species in East Asia. Specifically, we evaluated cryoprotectant type, concentration, and cooling rate and found that the highest fertilization (56–61%) and hatch (42–47%) can be obtained from yellow drum sperm suspended in 5% ethylene glycol or 10% dimethyl sulfoxide, cooled at 220°C/min, thawed in 40°C water bath for 7s, and fertilized with fresh (non-frozen) eggs at a sperm-to-egg ratio of 10⁷. We also compared other sperm quality parameters such as sperm motility, motility duration, membrane integrity, mitochondrial membrane potential, and DNA fragmentation between fresh and thawed sperm samples. Although cryopreservation resulted in a significant increase in DNA fragmentation and significant decreases in the remaining parameters, the magnitude of these changes was small when compared to the 2000 to 3000-fold reduction in fertilization/hatch capability of thawed sperm. These findings suggest possible disruption of signaling pathways that cannot be detected by conventional quality assays. Further analysis of sperm membrane lipid composition revealed a significant negative correlation between sperm quality and cholesterol-to-sphingomyelin ratio. These findings indicate that lipid rafts could be the main targets for cryodamage-induced decrease in fertilization capability of thawed sperm. Future studies are necessary to further explore the role of lipid rafts in cryopreservation-mediated damage in fish sperm. ; p. 82-88.
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authorDai , Tongren ; Zhao , Enhui ; Lu , Gang ; Che , Kai ; He , Qiutao ; Lu , Yonglin ; Fang , Qiongshan ; Wang , Hansheng ; Zheng , Leyun ; Li , Suping ; Huang , Changjiang ; Dong , Qiaoxiang
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abstractThis study was intended to develop an optimal sperm cryopreservation protocol for yellow drum (Nibea albiflora), a commercially important fish species in East Asia. Specifically, we evaluated cryoprotectant type, concentration, and cooling rate and found that the highest fertilization (56–61%) and hatch (42–47%) can be obtained from yellow drum sperm suspended in 5% ethylene glycol or 10% dimethyl sulfoxide, cooled at 220°C/min, thawed in 40°C water bath for 7s, and fertilized with fresh (non-frozen) eggs at a sperm-to-egg ratio of 10⁷. We also compared other sperm quality parameters such as sperm motility, motility duration, membrane integrity, mitochondrial membrane potential, and DNA fragmentation between fresh and thawed sperm samples. Although cryopreservation resulted in a significant increase in DNA fragmentation and significant decreases in the remaining parameters, the magnitude of these changes was small when compared to the 2000 to 3000-fold reduction in fertilization/hatch capability of thawed sperm. These findings suggest possible disruption of signaling pathways that cannot be detected by conventional quality assays. Further analysis of sperm membrane lipid composition revealed a significant negative correlation between sperm quality and cholesterol-to-sphingomyelin ratio. These findings indicate that lipid rafts could be the main targets for cryodamage-induced decrease in fertilization capability of thawed sperm. Future studies are necessary to further explore the role of lipid rafts in cryopreservation-mediated damage in fish sperm.
pubElsevier B.V.