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cAMP response element-binding protein promotes gliomagenesis by modulating the expression of oncogenic microRNA-23a

Gliomas are the most common and deadly type of primary brain tumor. In this study, we showed that cAMP response element-binding protein (CREB), a proto-oncogenic transcription factor that is overexpressed in gliomas, can promote gliomagenesis by modulating the expression of oncogenic microRNA-23a (m... Full description

Journal Title: Proceedings of the National Academy of Sciences of the United States of America 2012, Vol.109(39), pp.15805-15810
Main Author: Tan , Xiaochao
Other Authors: Wang , Shan , Zhu , Liyuan , Wu , Chao , Yin , Bin , Zhao , Jizong , Yuan , Jiangang , Qiang , Boqin , Peng , Xiaozhong
Format: Electronic Article Electronic Article
Language: English
Subjects:
Publisher: National Academy of Sciences
Created: 2012
ID: ISSN: 0027-8424
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recordid: faoagrisUS201600129815
title: cAMP response element-binding protein promotes gliomagenesis by modulating the expression of oncogenic microRNA-23a
format: Article
creator:
  • Tan , Xiaochao
  • Wang , Shan
  • Zhu , Liyuan
  • Wu , Chao
  • Yin , Bin
  • Zhao , Jizong
  • Yuan , Jiangang
  • Qiang , Boqin
  • Peng , Xiaozhong
subjects:
  • Microarray Technology
  • Cell Viability
  • Cyclic Amp
  • Gene Overexpression
  • Prediction
  • Transcription Factors
  • Regulatory Sequences
  • Microrna
  • Cell Growth
  • Brain
  • Polymerase Chain Reaction
  • Luciferase
  • Tissues
ispartof: Proceedings of the National Academy of Sciences of the United States of America, 2012, Vol.109(39), pp.15805-15810
description: Gliomas are the most common and deadly type of primary brain tumor. In this study, we showed that cAMP response element-binding protein (CREB), a proto-oncogenic transcription factor that is overexpressed in gliomas, can promote gliomagenesis by modulating the expression of oncogenic microRNA-23a (mir-23a). First, we found that CREB is highly expressed in glioma tissues and cell lines. CREB is also essential for glioma cell growth and cell survival in vitro and is critical for gliomagenesis in vivo. Second, microRNA microarray, ChIP-chip, ChIP-quantitative PCR, and luciferase reporter assays showed that CREB directly binds to the regulatory sequences of mir-23a and enhance the expression of mir-23a. Moreover, mir-23a was confirmed as a functional downstream target of CREB in glioma cell growth and cell survival. Finally, using computational prediction followed by experimental confirmation, we identified PTEN, which is frequently silenced in gliomas, as a downstream target of mir-23a. Taken together, we propose that CREB promotes gliomagenesis and acts as a modulator of oncogenic mir-23a, which represses the tumor suppressor PTEN. ; p. 15805-15810.
language: eng
source:
identifier: ISSN: 0027-8424
fulltext: fulltext
issn:
  • 00278424
  • 0027-8424
url: Link


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titlecAMP response element-binding protein promotes gliomagenesis by modulating the expression of oncogenic microRNA-23a
creatorTan , Xiaochao ; Wang , Shan ; Zhu , Liyuan ; Wu , Chao ; Yin , Bin ; Zhao , Jizong ; Yuan , Jiangang ; Qiang , Boqin ; Peng , Xiaozhong
publisherNational Academy of Sciences
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ispartofProceedings of the National Academy of Sciences of the United States of America, 2012, Vol.109(39), pp.15805-15810
identifierISSN: 0027-8424
subjectMicroarray Technology ; Cell Viability ; Cyclic Amp ; Gene Overexpression ; Prediction ; Transcription Factors ; Regulatory Sequences ; Microrna ; Cell Growth ; Brain ; Polymerase Chain Reaction ; Luciferase ; Tissues
descriptionGliomas are the most common and deadly type of primary brain tumor. In this study, we showed that cAMP response element-binding protein (CREB), a proto-oncogenic transcription factor that is overexpressed in gliomas, can promote gliomagenesis by modulating the expression of oncogenic microRNA-23a (mir-23a). First, we found that CREB is highly expressed in glioma tissues and cell lines. CREB is also essential for glioma cell growth and cell survival in vitro and is critical for gliomagenesis in vivo. Second, microRNA microarray, ChIP-chip, ChIP-quantitative PCR, and luciferase reporter assays showed that CREB directly binds to the regulatory sequences of mir-23a and enhance the expression of mir-23a. Moreover, mir-23a was confirmed as a functional downstream target of CREB in glioma cell growth and cell survival. Finally, using computational prediction followed by experimental confirmation, we identified PTEN, which is frequently silenced in gliomas, as a downstream target of mir-23a. Taken together, we propose that CREB promotes gliomagenesis and acts as a modulator of oncogenic mir-23a, which represses the tumor suppressor PTEN. ; p. 15805-15810.
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titlecAMP response element-binding protein promotes gliomagenesis by modulating the expression of oncogenic microRNA-23a
descriptionGliomas are the most common and deadly type of primary brain tumor. In this study, we showed that cAMP response element-binding protein (CREB), a proto-oncogenic transcription factor that is overexpressed in gliomas, can promote gliomagenesis by modulating the expression of oncogenic microRNA-23a (mir-23a). First, we found that CREB is highly expressed in glioma tissues and cell lines. CREB is also essential for glioma cell growth and cell survival in vitro and is critical for gliomagenesis in vivo. Second, microRNA microarray, ChIP-chip, ChIP-quantitative PCR, and luciferase reporter assays showed that CREB directly binds to the regulatory sequences of mir-23a and enhance the expression of mir-23a. Moreover, mir-23a was confirmed as a functional downstream target of CREB in glioma cell growth and cell survival. Finally, using computational prediction followed by experimental confirmation, we identified PTEN, which is frequently silenced in gliomas, as a downstream target of mir-23a. Taken together, we propose that CREB promotes gliomagenesis and acts as a modulator of oncogenic mir-23a, which represses the tumor suppressor PTEN. ; p. 15805-15810.
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abstractGliomas are the most common and deadly type of primary brain tumor. In this study, we showed that cAMP response element-binding protein (CREB), a proto-oncogenic transcription factor that is overexpressed in gliomas, can promote gliomagenesis by modulating the expression of oncogenic microRNA-23a (mir-23a). First, we found that CREB is highly expressed in glioma tissues and cell lines. CREB is also essential for glioma cell growth and cell survival in vitro and is critical for gliomagenesis in vivo. Second, microRNA microarray, ChIP-chip, ChIP-quantitative PCR, and luciferase reporter assays showed that CREB directly binds to the regulatory sequences of mir-23a and enhance the expression of mir-23a. Moreover, mir-23a was confirmed as a functional downstream target of CREB in glioma cell growth and cell survival. Finally, using computational prediction followed by experimental confirmation, we identified PTEN, which is frequently silenced in gliomas, as a downstream target of mir-23a. Taken together, we propose that CREB promotes gliomagenesis and acts as a modulator of oncogenic mir-23a, which represses the tumor suppressor PTEN.
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