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In situ precipitation: a novel approach for preparation of iron-oxide magnetoliposomes.(ORIGINAL RESEARCH)(Report)

Background: Conventional methods of preparing magnetoliposomes are complicated and inefficient. A novel approach for magnetoliposomes preparation was investigated in the study reported here. Methods: Fe[Cl.sub.3]/Fe[Cl.sub.2], solutions were hydrated with lipid films to obtain liposome-encapsulated... Full description

Journal Title: International Journal of Nanomedicine Annual, 2014, Vol.9, p.2607(11)
Main Author: Xia, Shudong
Other Authors: Li, Peng , Chen, Qiang , Armah, Malik , Ying, Xiaoying , Wu, Jian , Lai, Jiangtao
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 1178-2013 ; DOI: 10.2147/IJN.S59859
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recordid: gale_hrca401216844
title: In situ precipitation: a novel approach for preparation of iron-oxide magnetoliposomes.(ORIGINAL RESEARCH)(Report)
format: Article
creator:
  • Xia, Shudong
  • Li, Peng
  • Chen, Qiang
  • Armah, Malik
  • Ying, Xiaoying
  • Wu, Jian
  • Lai, Jiangtao
subjects:
  • Drug Delivery Systems -- Research
  • Ferric Oxide -- Usage
  • Ferric Oxide -- Properties
  • Ferric Oxide -- Research
  • Liposomes -- Usage
  • Liposomes -- Properties
  • Liposomes -- Research
ispartof: International Journal of Nanomedicine, Annual, 2014, Vol.9, p.2607(11)
description: Background: Conventional methods of preparing magnetoliposomes are complicated and inefficient. A novel approach for magnetoliposomes preparation was investigated in the study reported here. Methods: Fe[Cl.sub.3]/Fe[Cl.sub.2], solutions were hydrated with lipid films to obtain liposome-encapsulated iron ions by ultrasonic dispersion. Non-encapsulated iron ions were removed by dialysis. N[H.sub.3] x [H.sub.2]O was added to the system to adjust the pH to a critical value. Four different systems were prepared. Each was incubated at a different temperature for a different length of time to facilitate the permeation of N[H.sub.3] x [H.sub.2]O into the inner phase of the liposomes and the in situ formation of magnetic iron-oxide cores in the liposomes. Single-factor analysis and orthogonal-design experiments were applied to determinate the effects of alkalization pH, temperature, duration, and initial Fe concentration on encapsulation efficiency and drug loading. Results: The magnetoliposomes prepared by in situ precipitation had an average particle size of 168 [+ or -] 14 nm, zeta potential of -26.2 [+ or -] 1.9 mV and polydispersity index of 0.23 [+ or -] 0.06. The iron-oxide cores were confirmed as [Fe.sub.3][O.sub.4] by X-ray diffraction and demonstrated a superparamagnetic response. Encapsulation efficiency ranged from 3% to 22%, while drug loading ranged from 0.2 to 1.58 mol Fe/mol lipid. The optimal conditions for in situ precipitation were found to be an alkalization pH of 12, temperature of 60[degrees]C, time of 60 minutes, and initial Fe concentration of 100 mM [Fe.sup.3+] + 50 mM [Fe.sup.2+]. Conclusion: In situ precipitation could be a simple and efficient approach for the preparation of iron-oxide magnetoliposomes. Keywords: magnetoliposomes, in situ precipitation, iron oxide, alkalization, permeability
language: English
source:
identifier: ISSN: 1178-2013 ; DOI: 10.2147/IJN.S59859
fulltext: fulltext
issn:
  • 1178-2013
  • 11782013
url: Link


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titleIn situ precipitation: a novel approach for preparation of iron-oxide magnetoliposomes.(ORIGINAL RESEARCH)(Report)
creatorXia, Shudong ; Li, Peng ; Chen, Qiang ; Armah, Malik ; Ying, Xiaoying ; Wu, Jian ; Lai, Jiangtao
ispartofInternational Journal of Nanomedicine, Annual, 2014, Vol.9, p.2607(11)
identifierISSN: 1178-2013 ; DOI: 10.2147/IJN.S59859
subjectDrug Delivery Systems -- Research ; Ferric Oxide -- Usage ; Ferric Oxide -- Properties ; Ferric Oxide -- Research ; Liposomes -- Usage ; Liposomes -- Properties ; Liposomes -- Research
descriptionBackground: Conventional methods of preparing magnetoliposomes are complicated and inefficient. A novel approach for magnetoliposomes preparation was investigated in the study reported here. Methods: Fe[Cl.sub.3]/Fe[Cl.sub.2], solutions were hydrated with lipid films to obtain liposome-encapsulated iron ions by ultrasonic dispersion. Non-encapsulated iron ions were removed by dialysis. N[H.sub.3] x [H.sub.2]O was added to the system to adjust the pH to a critical value. Four different systems were prepared. Each was incubated at a different temperature for a different length of time to facilitate the permeation of N[H.sub.3] x [H.sub.2]O into the inner phase of the liposomes and the in situ formation of magnetic iron-oxide cores in the liposomes. Single-factor analysis and orthogonal-design experiments were applied to determinate the effects of alkalization pH, temperature, duration, and initial Fe concentration on encapsulation efficiency and drug loading. Results: The magnetoliposomes prepared by in situ precipitation had an average particle size of 168 [+ or -] 14 nm, zeta potential of -26.2 [+ or -] 1.9 mV and polydispersity index of 0.23 [+ or -] 0.06. The iron-oxide cores were confirmed as [Fe.sub.3][O.sub.4] by X-ray diffraction and demonstrated a superparamagnetic response. Encapsulation efficiency ranged from 3% to 22%, while drug loading ranged from 0.2 to 1.58 mol Fe/mol lipid. The optimal conditions for in situ precipitation were found to be an alkalization pH of 12, temperature of 60[degrees]C, time of 60 minutes, and initial Fe concentration of 100 mM [Fe.sup.3+] + 50 mM [Fe.sup.2+]. Conclusion: In situ precipitation could be a simple and efficient approach for the preparation of iron-oxide magnetoliposomes. Keywords: magnetoliposomes, in situ precipitation, iron oxide, alkalization, permeability
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titleIn situ precipitation: a novel approach for preparation of iron-oxide magnetoliposomes.(ORIGINAL RESEARCH)(Report)
descriptionBackground: Conventional methods of preparing magnetoliposomes are complicated and inefficient. A novel approach for magnetoliposomes preparation was investigated in the study reported here. Methods: Fe[Cl.sub.3]/Fe[Cl.sub.2], solutions were hydrated with lipid films to obtain liposome-encapsulated iron ions by ultrasonic dispersion. Non-encapsulated iron ions were removed by dialysis. N[H.sub.3] x [H.sub.2]O was added to the system to adjust the pH to a critical value. Four different systems were prepared. Each was incubated at a different temperature for a different length of time to facilitate the permeation of N[H.sub.3] x [H.sub.2]O into the inner phase of the liposomes and the in situ formation of magnetic iron-oxide cores in the liposomes. Single-factor analysis and orthogonal-design experiments were applied to determinate the effects of alkalization pH, temperature, duration, and initial Fe concentration on encapsulation efficiency and drug loading. Results: The magnetoliposomes prepared by in situ precipitation had an average particle size of 168 [+ or -] 14 nm, zeta potential of -26.2 [+ or -] 1.9 mV and polydispersity index of 0.23 [+ or -] 0.06. The iron-oxide cores were confirmed as [Fe.sub.3][O.sub.4] by X-ray diffraction and demonstrated a superparamagnetic response. Encapsulation efficiency ranged from 3% to 22%, while drug loading ranged from 0.2 to 1.58 mol Fe/mol lipid. The optimal conditions for in situ precipitation were found to be an alkalization pH of 12, temperature of 60[degrees]C, time of 60 minutes, and initial Fe concentration of 100 mM [Fe.sup.3+] + 50 mM [Fe.sup.2+]. Conclusion: In situ precipitation could be a simple and efficient approach for the preparation of iron-oxide magnetoliposomes. Keywords: magnetoliposomes, in situ precipitation, iron oxide, alkalization, permeability
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titleIn situ precipitation: a novel approach for preparation of iron-oxide magnetoliposomes.(ORIGINAL RESEARCH)(Report)
authorXia, Shudong ; Li, Peng ; Chen, Qiang ; Armah, Malik ; Ying, Xiaoying ; Wu, Jian ; Lai, Jiangtao
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abstractBackground: Conventional methods of preparing magnetoliposomes are complicated and inefficient. A novel approach for magnetoliposomes preparation was investigated in the study reported here. Methods: Fe[Cl.sub.3]/Fe[Cl.sub.2], solutions were hydrated with lipid films to obtain liposome-encapsulated iron ions by ultrasonic dispersion. Non-encapsulated iron ions were removed by dialysis. N[H.sub.3] x [H.sub.2]O was added to the system to adjust the pH to a critical value. Four different systems were prepared. Each was incubated at a different temperature for a different length of time to facilitate the permeation of N[H.sub.3] x [H.sub.2]O into the inner phase of the liposomes and the in situ formation of magnetic iron-oxide cores in the liposomes. Single-factor analysis and orthogonal-design experiments were applied to determinate the effects of alkalization pH, temperature, duration, and initial Fe concentration on encapsulation efficiency and drug loading. Results: The magnetoliposomes prepared by in situ precipitation had an average particle size of 168 [+ or -] 14 nm, zeta potential of -26.2 [+ or -] 1.9 mV and polydispersity index of 0.23 [+ or -] 0.06. The iron-oxide cores were confirmed as [Fe.sub.3][O.sub.4] by X-ray diffraction and demonstrated a superparamagnetic response. Encapsulation efficiency ranged from 3% to 22%, while drug loading ranged from 0.2 to 1.58 mol Fe/mol lipid. The optimal conditions for in situ precipitation were found to be an alkalization pH of 12, temperature of 60[degrees]C, time of 60 minutes, and initial Fe concentration of 100 mM [Fe.sup.3+] + 50 mM [Fe.sup.2+]. Conclusion: In situ precipitation could be a simple and efficient approach for the preparation of iron-oxide magnetoliposomes. Keywords: magnetoliposomes, in situ precipitation, iron oxide, alkalization, permeability
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date2014-01-01