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Analysis of the Microbiota of Black Stain in the Primary Dentition.(Report)

Black tooth stain is a characteristic extrinsic discoloration commonly seen on the cervical enamel following the contour of the gingiva. To investigate the relationship between black tooth stain and the oral microbiota, we used 16S rRNA gene sequencing to compare the microbial composition of dental... Full description

Journal Title: PLoS ONE Sept 4, 2015, Vol.10(9)
Main Author: Li, Yue
Other Authors: Zhang, Qian , Zhang, Fangfei , Liu, Ruoxi , Liu, He , Chen, Feng
Format: Electronic Article Electronic Article
Language: English
Subjects:
Quelle: Cengage Learning, Inc.
ID: ISSN: 1932-6203 ; DOI: 10.1371/journal.pone.0137030
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recordid: gale_hrca427722829
title: Analysis of the Microbiota of Black Stain in the Primary Dentition.(Report)
format: Article
creator:
  • Li, Yue
  • Zhang, Qian
  • Zhang, Fangfei
  • Liu, Ruoxi
  • Liu, He
  • Chen, Feng
subjects:
  • Microbiota (Symbiotic Organisms) -- Physiological Aspects
  • Microbiota (Symbiotic Organisms) -- Genetic Aspects
  • Microbiota (Symbiotic Organisms) -- Research
  • Dentition -- Physiological Aspects
  • Dentition -- Genetic Aspects
  • Dentition -- Research
ispartof: PLoS ONE, Sept 4, 2015, Vol.10(9)
description: Black tooth stain is a characteristic extrinsic discoloration commonly seen on the cervical enamel following the contour of the gingiva. To investigate the relationship between black tooth stain and the oral microbiota, we used 16S rRNA gene sequencing to compare the microbial composition of dental plaque and saliva among caries-free children with and without black stain. Dental plaque and saliva, as well as black stain, were sampled from 10 children with and 15 children without black stain. Data were analyzed using the pipeline tool MOTHUR. Student's t-test was used to compare alpha diversities and the Mann-Whitney U test to compare the relative abundances of the microbial taxa. A total of 10 phyla, 19 classes, 32 orders, 61 families and 102 genera were detected in these samples. Shannon and Simpson diversity were found to be significantly lower in saliva samples of children with black stain. Microbial diversity was reduced in the black stain compared to the plaque samples. Actinomyces, Cardiobacterium, Haemophilus, Corynebacterium, Tannerella and Treponema were more abundant and Campylobacter less abundant in plaque samples of children with black stain. Principal component analysis demonstrated clustering among the dental plaque samples from the control group, while the plaque samples from the black stain group were not and appeared to cluster into two subgroups. Alterations in oral microbiota may be associated with the formation of black stain.
language: English
source: Cengage Learning, Inc.
identifier: ISSN: 1932-6203 ; DOI: 10.1371/journal.pone.0137030
fulltext: fulltext
issn:
  • 1932-6203
  • 19326203
url: Link


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titleAnalysis of the Microbiota of Black Stain in the Primary Dentition.(Report)
creatorLi, Yue ; Zhang, Qian ; Zhang, Fangfei ; Liu, Ruoxi ; Liu, He ; Chen, Feng
ispartofPLoS ONE, Sept 4, 2015, Vol.10(9)
identifierISSN: 1932-6203 ; DOI: 10.1371/journal.pone.0137030
subjectMicrobiota (Symbiotic Organisms) -- Physiological Aspects ; Microbiota (Symbiotic Organisms) -- Genetic Aspects ; Microbiota (Symbiotic Organisms) -- Research ; Dentition -- Physiological Aspects ; Dentition -- Genetic Aspects ; Dentition -- Research
descriptionBlack tooth stain is a characteristic extrinsic discoloration commonly seen on the cervical enamel following the contour of the gingiva. To investigate the relationship between black tooth stain and the oral microbiota, we used 16S rRNA gene sequencing to compare the microbial composition of dental plaque and saliva among caries-free children with and without black stain. Dental plaque and saliva, as well as black stain, were sampled from 10 children with and 15 children without black stain. Data were analyzed using the pipeline tool MOTHUR. Student's t-test was used to compare alpha diversities and the Mann-Whitney U test to compare the relative abundances of the microbial taxa. A total of 10 phyla, 19 classes, 32 orders, 61 families and 102 genera were detected in these samples. Shannon and Simpson diversity were found to be significantly lower in saliva samples of children with black stain. Microbial diversity was reduced in the black stain compared to the plaque samples. Actinomyces, Cardiobacterium, Haemophilus, Corynebacterium, Tannerella and Treponema were more abundant and Campylobacter less abundant in plaque samples of children with black stain. Principal component analysis demonstrated clustering among the dental plaque samples from the control group, while the plaque samples from the black stain group were not and appeared to cluster into two subgroups. Alterations in oral microbiota may be associated with the formation of black stain.
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titleAnalysis of the Microbiota of Black Stain in the Primary Dentition.(Report)
descriptionBlack tooth stain is a characteristic extrinsic discoloration commonly seen on the cervical enamel following the contour of the gingiva. To investigate the relationship between black tooth stain and the oral microbiota, we used 16S rRNA gene sequencing to compare the microbial composition of dental plaque and saliva among caries-free children with and without black stain. Dental plaque and saliva, as well as black stain, were sampled from 10 children with and 15 children without black stain. Data were analyzed using the pipeline tool MOTHUR. Student's t-test was used to compare alpha diversities and the Mann-Whitney U test to compare the relative abundances of the microbial taxa. A total of 10 phyla, 19 classes, 32 orders, 61 families and 102 genera were detected in these samples. Shannon and Simpson diversity were found to be significantly lower in saliva samples of children with black stain. Microbial diversity was reduced in the black stain compared to the plaque samples. Actinomyces, Cardiobacterium, Haemophilus, Corynebacterium, Tannerella and Treponema were more abundant and Campylobacter less abundant in plaque samples of children with black stain. Principal component analysis demonstrated clustering among the dental plaque samples from the control group, while the plaque samples from the black stain group were not and appeared to cluster into two subgroups. Alterations in oral microbiota may be associated with the formation of black stain.
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abstractBlack tooth stain is a characteristic extrinsic discoloration commonly seen on the cervical enamel following the contour of the gingiva. To investigate the relationship between black tooth stain and the oral microbiota, we used 16S rRNA gene sequencing to compare the microbial composition of dental plaque and saliva among caries-free children with and without black stain. Dental plaque and saliva, as well as black stain, were sampled from 10 children with and 15 children without black stain. Data were analyzed using the pipeline tool MOTHUR. Student's t-test was used to compare alpha diversities and the Mann-Whitney U test to compare the relative abundances of the microbial taxa. A total of 10 phyla, 19 classes, 32 orders, 61 families and 102 genera were detected in these samples. Shannon and Simpson diversity were found to be significantly lower in saliva samples of children with black stain. Microbial diversity was reduced in the black stain compared to the plaque samples. Actinomyces, Cardiobacterium, Haemophilus, Corynebacterium, Tannerella and Treponema were more abundant and Campylobacter less abundant in plaque samples of children with black stain. Principal component analysis demonstrated clustering among the dental plaque samples from the control group, while the plaque samples from the black stain group were not and appeared to cluster into two subgroups. Alterations in oral microbiota may be associated with the formation of black stain.
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doi10.1371/journal.pone.0137030
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