schliessen

Filtern

 

Bibliotheken

Technical Insights into Highly Sensitive Isolation and Molecular Characterization of Fixed and Live Circulating Tumor Cells for Early Detection of Tumor Invasion.(Research Article)

Circulating Tumor Cells (CTC) and Circulating Tumor Microemboli (CTM) are Circulating Rare Cells (CRC) which herald tumor invasion and are expected to provide an opportunity to improve the management of cancer patients. An unsolved technical issue in the CTC field is how to obtain highly sensitive a... Full description

Journal Title: PLoS ONE Jan 6, 2017, Vol.12(1), p.e0169427
Main Author: Laget, Sophie
Other Authors: Broncy, Lucile , Hormigos, Katia , Dhingra, Dalia M. , Benmohamed, Fatima , Capiod, Thierry , Osteras, Magne , Farinelli, Laurent , Jackson, Stephen , Paterlini - Brechot, Patrizia
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 1932-6203 ; DOI: 10.1371/journal.pone.0169427
Zum Text:
SendSend as email Add to Book BagAdd to Book Bag
Staff View
recordid: gale_hrca476711097
title: Technical Insights into Highly Sensitive Isolation and Molecular Characterization of Fixed and Live Circulating Tumor Cells for Early Detection of Tumor Invasion.(Research Article)
format: Article
creator:
  • Laget, Sophie
  • Broncy, Lucile
  • Hormigos, Katia
  • Dhingra, Dalia M.
  • Benmohamed, Fatima
  • Capiod, Thierry
  • Osteras, Magne
  • Farinelli, Laurent
  • Jackson, Stephen
  • Paterlini - Brechot, Patrizia
subjects:
  • Antigens -- Analysis
  • Cancer Metastasis -- Analysis
ispartof: PLoS ONE, Jan 6, 2017, Vol.12(1), p.e0169427
description: Circulating Tumor Cells (CTC) and Circulating Tumor Microemboli (CTM) are Circulating Rare Cells (CRC) which herald tumor invasion and are expected to provide an opportunity to improve the management of cancer patients. An unsolved technical issue in the CTC field is how to obtain highly sensitive and unbiased collection of these fragile and heterogeneous cells, in both live and fixed form, for their molecular study when they are extremely rare, particularly at the beginning of the invasion process. We report on a new protocol to enrich from blood live CTC using ISET.sup.#174; (Isolation by SizE of Tumor/Trophoblastic Cells), an open system originally developed for marker-independent isolation of fixed tumor cells. We have assessed the impact of our new enrichment method on live tumor cells antigen expression, cytoskeleton structure, cell viability and ability to expand in culture. We have also explored the ISET.sup.#174; in vitro performance to collect intact fixed and live cancer cells by using spiking analyses with extremely low number of fluorescent cultured cells. We describe results consistently showing the feasibility of isolating fixed and live tumor cells with a Lower Limit of Detection (LLOD) of one cancer cell per 10 mL of blood and a sensitivity at LLOD ranging from 83 to 100%. This very high sensitivity threshold can be maintained when plasma is collected before tumor cells isolation. Finally, we have performed a comparative next generation sequencing (NGS) analysis of tumor cells before and after isolation from blood and culture. We established the feasibility of NGS analysis of single live and fixed tumor cells enriched from blood by our system. This study provides new protocols for detection and characterization of CTC collected from blood at the very early steps of tumor invasion.
language: English
source:
identifier: ISSN: 1932-6203 ; DOI: 10.1371/journal.pone.0169427
fulltext: fulltext
issn:
  • 1932-6203
  • 19326203
url: Link


@attributes
ID1391636480
RANK0.07
NO1
SEARCH_ENGINEprimo_central_multiple_fe
SEARCH_ENGINE_TYPEPrimo Central Search Engine
LOCALfalse
PrimoNMBib
record
control
sourcerecordid476711097
sourceidgale_hrca
recordidTN_gale_hrca476711097
sourceformatXML
sourcesystemPC
pqid1856128632
galeid476711097
display
typearticle
titleTechnical Insights into Highly Sensitive Isolation and Molecular Characterization of Fixed and Live Circulating Tumor Cells for Early Detection of Tumor Invasion.(Research Article)
creatorLaget, Sophie ; Broncy, Lucile ; Hormigos, Katia ; Dhingra, Dalia M. ; Benmohamed, Fatima ; Capiod, Thierry ; Osteras, Magne ; Farinelli, Laurent ; Jackson, Stephen ; Paterlini - Brechot, Patrizia
ispartofPLoS ONE, Jan 6, 2017, Vol.12(1), p.e0169427
identifierISSN: 1932-6203 ; DOI: 10.1371/journal.pone.0169427
subjectAntigens -- Analysis ; Cancer Metastasis -- Analysis
descriptionCirculating Tumor Cells (CTC) and Circulating Tumor Microemboli (CTM) are Circulating Rare Cells (CRC) which herald tumor invasion and are expected to provide an opportunity to improve the management of cancer patients. An unsolved technical issue in the CTC field is how to obtain highly sensitive and unbiased collection of these fragile and heterogeneous cells, in both live and fixed form, for their molecular study when they are extremely rare, particularly at the beginning of the invasion process. We report on a new protocol to enrich from blood live CTC using ISET.sup.#174; (Isolation by SizE of Tumor/Trophoblastic Cells), an open system originally developed for marker-independent isolation of fixed tumor cells. We have assessed the impact of our new enrichment method on live tumor cells antigen expression, cytoskeleton structure, cell viability and ability to expand in culture. We have also explored the ISET.sup.#174; in vitro performance to collect intact fixed and live cancer cells by using spiking analyses with extremely low number of fluorescent cultured cells. We describe results consistently showing the feasibility of isolating fixed and live tumor cells with a Lower Limit of Detection (LLOD) of one cancer cell per 10 mL of blood and a sensitivity at LLOD ranging from 83 to 100%. This very high sensitivity threshold can be maintained when plasma is collected before tumor cells isolation. Finally, we have performed a comparative next generation sequencing (NGS) analysis of tumor cells before and after isolation from blood and culture. We established the feasibility of NGS analysis of single live and fixed tumor cells enriched from blood by our system. This study provides new protocols for detection and characterization of CTC collected from blood at the very early steps of tumor invasion.
languageEnglish
source
version9
lds50peer_reviewed
links
openurl$$Topenurl_article
openurlfulltext$$Topenurlfull_article
search
creatorcontrib
0Laget, Sophie
1Sophie Laget; Lucile Broncy; Katia Hormigos; Dalia M. Dhingra; Fatima BenMohamed; Thierry Capiod; Magne Osteras; Laurent Farinelli; Stephen Jackson; Patrizia Paterlini-Brechot
2Broncy, Lucile
3Hormigos, Katia
4Dhingra, Dalia M
5BenMohamed, Fatima
6Capiod, Thierry
7Osteras, Magne
8Farinelli, Laurent
9Jackson, Stephen
10Paterlini-Brechot, Patrizia
titleTechnical Insights into Highly Sensitive Isolation and Molecular Characterization of Fixed and Live Circulating Tumor Cells for Early Detection of Tumor Invasion.(Research Article)
descriptionCirculating Tumor Cells (CTC) and Circulating Tumor Microemboli (CTM) are Circulating Rare Cells (CRC) which herald tumor invasion and are expected to provide an opportunity to improve the management of cancer patients. An unsolved technical issue in the CTC field is how to obtain highly sensitive and unbiased collection of these fragile and heterogeneous cells, in both live and fixed form, for their molecular study when they are extremely rare, particularly at the beginning of the invasion process. We report on a new protocol to enrich from blood live CTC using ISET.sup.#174; (Isolation by SizE of Tumor/Trophoblastic Cells), an open system originally developed for marker-independent isolation of fixed tumor cells. We have assessed the impact of our new enrichment method on live tumor cells antigen expression, cytoskeleton structure, cell viability and ability to expand in culture. We have also explored the ISET.sup.#174; in vitro performance to collect intact fixed and live cancer cells by using spiking analyses with extremely low number of fluorescent cultured cells. We describe results consistently showing the feasibility of isolating fixed and live tumor cells with a Lower Limit of Detection (LLOD) of one cancer cell per 10 mL of blood and a sensitivity at LLOD ranging from 83 to 100%. This very high sensitivity threshold can be maintained when plasma is collected before tumor cells isolation. Finally, we have performed a comparative next generation sequencing (NGS) analysis of tumor cells before and after isolation from blood and culture. We established the feasibility of NGS analysis of single live and fixed tumor cells enriched from blood by our system. This study provides new protocols for detection and characterization of CTC collected from blood at the very early steps of tumor invasion.
subject
0Antigens--Analysis
1Cancer metastasis--Analysis
22836
3Biological products exc. diagnostic
general
0English
1Public Library of Science
2Cengage Learning, Inc.
sourceidgale_hrca
recordidgale_hrca476711097
issn
01932-6203
119326203
rsrctypearticle
creationdate2017
recordtypearticle
addtitlePLoS ONE
searchscopegale_hrca
scopegale_hrca
lsr30VSR-Enriched:[galeid, pqid, pages]
sort
titleTechnical Insights into Highly Sensitive Isolation and Molecular Characterization of Fixed and Live Circulating Tumor Cells for Early Detection of Tumor Invasion.(Research Article)
authorLaget, Sophie ; Broncy, Lucile ; Hormigos, Katia ; Dhingra, Dalia M. ; Benmohamed, Fatima ; Capiod, Thierry ; Osteras, Magne ; Farinelli, Laurent ; Jackson, Stephen ; Paterlini - Brechot, Patrizia
creationdate20170106
facets
frbrgroupid8881749740075476107
frbrtype5
newrecords20170117
languageeng
creationdate2017
topic
0Antigens–Analysis
1Cancer Metastasis–Analysis
collectionHealth Reference Center Academic (Gale)
prefilterarticles
rsrctypearticles
creatorcontrib
0Laget, Sophie
1Broncy, Lucile
2Hormigos, Katia
3Dhingra, Dalia M.
4Benmohamed, Fatima
5Capiod, Thierry
6Osteras, Magne
7Farinelli, Laurent
8Jackson, Stephen
9Paterlini - Brechot, Patrizia
jtitlePLoS ONE
toplevelpeer_reviewed
delivery
delcategoryRemote Search Resource
fulltextfulltext
addata
au
0Laget, Sophie
1Broncy, Lucile
2Hormigos, Katia
3Dhingra, Dalia M.
4BenMohamed, Fatima
5Capiod, Thierry
6Osteras, Magne
7Farinelli, Laurent
8Jackson, Stephen
9Paterlini-Brechot, Patrizia
atitleTechnical Insights into Highly Sensitive Isolation and Molecular Characterization of Fixed and Live Circulating Tumor Cells for Early Detection of Tumor Invasion
jtitlePLoS ONE
risdate20170106
volume12
issue1
spagee0169427
issn1932-6203
genrearticle
ristypeJOUR
abstractCirculating Tumor Cells (CTC) and Circulating Tumor Microemboli (CTM) are Circulating Rare Cells (CRC) which herald tumor invasion and are expected to provide an opportunity to improve the management of cancer patients. An unsolved technical issue in the CTC field is how to obtain highly sensitive and unbiased collection of these fragile and heterogeneous cells, in both live and fixed form, for their molecular study when they are extremely rare, particularly at the beginning of the invasion process. We report on a new protocol to enrich from blood live CTC using ISET.sup.#174; (Isolation by SizE of Tumor/Trophoblastic Cells), an open system originally developed for marker-independent isolation of fixed tumor cells. We have assessed the impact of our new enrichment method on live tumor cells antigen expression, cytoskeleton structure, cell viability and ability to expand in culture. We have also explored the ISET.sup.#174; in vitro performance to collect intact fixed and live cancer cells by using spiking analyses with extremely low number of fluorescent cultured cells. We describe results consistently showing the feasibility of isolating fixed and live tumor cells with a Lower Limit of Detection (LLOD) of one cancer cell per 10 mL of blood and a sensitivity at LLOD ranging from 83 to 100%. This very high sensitivity threshold can be maintained when plasma is collected before tumor cells isolation. Finally, we have performed a comparative next generation sequencing (NGS) analysis of tumor cells before and after isolation from blood and culture. We established the feasibility of NGS analysis of single live and fixed tumor cells enriched from blood by our system. This study provides new protocols for detection and characterization of CTC collected from blood at the very early steps of tumor invasion.
pubPublic Library of Science
doi10.1371/journal.pone.0169427
lad01gale_hrca
pagese0169427
date2017-01-06