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SKP2 High Expression, KIT Exon 11 Deletions, and Gastrointestinal Bleeding as Predictors of Poor Prognosis in Primary Gastrointestinal Stromal Tumors.(Research Article)

Background and Aims Considering the indication of adjuvant therapy, the recurrence risk for primary gastrointestinal stromal tumor (GIST) after surgery needs to be accurately estimated. However, current risk stratification schemes may still have room for improvement. This study seeks to analyze prog... Full description

Journal Title: PLoS ONE May 17, 2013, Vol.8(5), p.e62951
Main Author: Lv, Ang
Other Authors: Li, Zhongwu , Tian, Xiuyun , Guan, Xiaoya , Zhao, Min , Dong, Bin , Hao, Chunyi
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 1932-6203 ; DOI: 10.1371/journal.pone.0062951
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recordid: gale_hrca478240973
title: SKP2 High Expression, KIT Exon 11 Deletions, and Gastrointestinal Bleeding as Predictors of Poor Prognosis in Primary Gastrointestinal Stromal Tumors.(Research Article)
format: Article
creator:
  • Lv, Ang
  • Li, Zhongwu
  • Tian, Xiuyun
  • Guan, Xiaoya
  • Zhao, Min
  • Dong, Bin
  • Hao, Chunyi
subjects:
  • Tumor Proteins -- Genetic Aspects
  • Tumor Proteins -- Prognosis
  • Tumor Proteins -- Risk Factors
  • Tumor Proteins -- Analysis
  • Muscle Proteins -- Analysis
  • Tumors -- Genetic Aspects
  • Tumors -- Prognosis
  • Tumors -- Risk Factors
  • Tumors -- Analysis
  • Adjuvant Chemotherapy -- Analysis
  • Medical Research -- Analysis
  • Medical Records -- Analysis
  • Gastrointestinal Hemorrhage -- Genetic Aspects
  • Gastrointestinal Hemorrhage -- Prognosis
  • Gastrointestinal Hemorrhage -- Risk Factors
  • Gastrointestinal Hemorrhage -- Analysis
ispartof: PLoS ONE, May 17, 2013, Vol.8(5), p.e62951
description: Background and Aims Considering the indication of adjuvant therapy, the recurrence risk for primary gastrointestinal stromal tumor (GIST) after surgery needs to be accurately estimated. However, current risk stratification schemes may still have room for improvement. This study seeks to analyze prognostic factors for primary GISTs from 3 aspects, including clinicopathological parameters, immunohistochemical biomarkers, and gene mutational status, and attempts to find novel valuable factors predicting the malignancy potential of GISTs. Methods Retrospective data from 114 primary GIST patients after R0 resection were collected. Clinicopathological data was obtained from medical records and re-evaluated. Immunohistochemical analysis was performed using the Tissue Microarray method for Ki67, p16, p27, p53, SKP2, CD133, and actin. KIT gene exons 9, 11, 13, and 17 and PDGFR[alpha] gene exons 12 and 18 were tested for mutations using PCR. Results Univariate analysis revealed the following factors as poor prognostic indicators for relapse-free survival with a median follow-up of 50 months: male gender, gastrointestinal bleeding, mitotic index >5/50HPFs, tumor size >5 cm, non-gastric site, necrosis, epithelioid or mixed cell type, surrounding tissue invasion, Ki67>5%, p16>20%, p53 index >10, SKP2>10%, and KIT exon 11 deletion. Besides mitotic index, tumor size and site, SKP2 high expression (RR = 2.91, 95% CI: 1.41-5.99, P = 0.004) and KIT exon 11 deletion (RR = 2.73, 95% CI: 1.04-7.16, P = 0.041) were also independent risk factors in multivariate analysis, with gastrointestinal bleeding also showing a trend towards significance (RR = 1.88, 95% CI: 0.98-3.64, P = 0.059). In addition, gastrointestinal bleeding and SKP2 high expression showed a good ability to stratify high-risk patients further. Conclusion Our results show that gastrointestinal bleeding, SKP2 high expression, and KIT exon 11 deletions may be useful indicators of high recurrence risk for primary GIST patients.
language: English
source:
identifier: ISSN: 1932-6203 ; DOI: 10.1371/journal.pone.0062951
fulltext: fulltext
issn:
  • 1932-6203
  • 19326203
url: Link


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titleSKP2 High Expression, KIT Exon 11 Deletions, and Gastrointestinal Bleeding as Predictors of Poor Prognosis in Primary Gastrointestinal Stromal Tumors.(Research Article)
creatorLv, Ang ; Li, Zhongwu ; Tian, Xiuyun ; Guan, Xiaoya ; Zhao, Min ; Dong, Bin ; Hao, Chunyi
ispartofPLoS ONE, May 17, 2013, Vol.8(5), p.e62951
identifierISSN: 1932-6203 ; DOI: 10.1371/journal.pone.0062951
subjectTumor Proteins -- Genetic Aspects ; Tumor Proteins -- Prognosis ; Tumor Proteins -- Risk Factors ; Tumor Proteins -- Analysis ; Muscle Proteins -- Analysis ; Tumors -- Genetic Aspects ; Tumors -- Prognosis ; Tumors -- Risk Factors ; Tumors -- Analysis ; Adjuvant Chemotherapy -- Analysis ; Medical Research -- Analysis ; Medical Records -- Analysis ; Gastrointestinal Hemorrhage -- Genetic Aspects ; Gastrointestinal Hemorrhage -- Prognosis ; Gastrointestinal Hemorrhage -- Risk Factors ; Gastrointestinal Hemorrhage -- Analysis
descriptionBackground and Aims Considering the indication of adjuvant therapy, the recurrence risk for primary gastrointestinal stromal tumor (GIST) after surgery needs to be accurately estimated. However, current risk stratification schemes may still have room for improvement. This study seeks to analyze prognostic factors for primary GISTs from 3 aspects, including clinicopathological parameters, immunohistochemical biomarkers, and gene mutational status, and attempts to find novel valuable factors predicting the malignancy potential of GISTs. Methods Retrospective data from 114 primary GIST patients after R0 resection were collected. Clinicopathological data was obtained from medical records and re-evaluated. Immunohistochemical analysis was performed using the Tissue Microarray method for Ki67, p16, p27, p53, SKP2, CD133, and actin. KIT gene exons 9, 11, 13, and 17 and PDGFR[alpha] gene exons 12 and 18 were tested for mutations using PCR. Results Univariate analysis revealed the following factors as poor prognostic indicators for relapse-free survival with a median follow-up of 50 months: male gender, gastrointestinal bleeding, mitotic index >5/50HPFs, tumor size >5 cm, non-gastric site, necrosis, epithelioid or mixed cell type, surrounding tissue invasion, Ki67>5%, p16>20%, p53 index >10, SKP2>10%, and KIT exon 11 deletion. Besides mitotic index, tumor size and site, SKP2 high expression (RR = 2.91, 95% CI: 1.41-5.99, P = 0.004) and KIT exon 11 deletion (RR = 2.73, 95% CI: 1.04-7.16, P = 0.041) were also independent risk factors in multivariate analysis, with gastrointestinal bleeding also showing a trend towards significance (RR = 1.88, 95% CI: 0.98-3.64, P = 0.059). In addition, gastrointestinal bleeding and SKP2 high expression showed a good ability to stratify high-risk patients further. Conclusion Our results show that gastrointestinal bleeding, SKP2 high expression, and KIT exon 11 deletions may be useful indicators of high recurrence risk for primary GIST patients.
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7Hao, Chunyi
titleSKP2 High Expression, KIT Exon 11 Deletions, and Gastrointestinal Bleeding as Predictors of Poor Prognosis in Primary Gastrointestinal Stromal Tumors.(Research Article)
descriptionBackground and Aims Considering the indication of adjuvant therapy, the recurrence risk for primary gastrointestinal stromal tumor (GIST) after surgery needs to be accurately estimated. However, current risk stratification schemes may still have room for improvement. This study seeks to analyze prognostic factors for primary GISTs from 3 aspects, including clinicopathological parameters, immunohistochemical biomarkers, and gene mutational status, and attempts to find novel valuable factors predicting the malignancy potential of GISTs. Methods Retrospective data from 114 primary GIST patients after R0 resection were collected. Clinicopathological data was obtained from medical records and re-evaluated. Immunohistochemical analysis was performed using the Tissue Microarray method for Ki67, p16, p27, p53, SKP2, CD133, and actin. KIT gene exons 9, 11, 13, and 17 and PDGFR[alpha] gene exons 12 and 18 were tested for mutations using PCR. Results Univariate analysis revealed the following factors as poor prognostic indicators for relapse-free survival with a median follow-up of 50 months: male gender, gastrointestinal bleeding, mitotic index >5/50HPFs, tumor size >5 cm, non-gastric site, necrosis, epithelioid or mixed cell type, surrounding tissue invasion, Ki67>5%, p16>20%, p53 index >10, SKP2>10%, and KIT exon 11 deletion. Besides mitotic index, tumor size and site, SKP2 high expression (RR = 2.91, 95% CI: 1.41-5.99, P = 0.004) and KIT exon 11 deletion (RR = 2.73, 95% CI: 1.04-7.16, P = 0.041) were also independent risk factors in multivariate analysis, with gastrointestinal bleeding also showing a trend towards significance (RR = 1.88, 95% CI: 0.98-3.64, P = 0.059). In addition, gastrointestinal bleeding and SKP2 high expression showed a good ability to stratify high-risk patients further. Conclusion Our results show that gastrointestinal bleeding, SKP2 high expression, and KIT exon 11 deletions may be useful indicators of high recurrence risk for primary GIST patients.
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14Gastrointestinal hemorrhage--Risk factors
15Gastrointestinal hemorrhage--Analysis
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titleSKP2 High Expression, KIT Exon 11 Deletions, and Gastrointestinal Bleeding as Predictors of Poor Prognosis in Primary Gastrointestinal Stromal Tumors.(Research Article)
authorLv, Ang ; Li, Zhongwu ; Tian, Xiuyun ; Guan, Xiaoya ; Zhao, Min ; Dong, Bin ; Hao, Chunyi
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3Tumor Proteins–Analysis
4Muscle Proteins–Analysis
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6Tumors–Prognosis
7Tumors–Risk Factors
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9Adjuvant Chemotherapy–Analysis
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abstractBackground and Aims Considering the indication of adjuvant therapy, the recurrence risk for primary gastrointestinal stromal tumor (GIST) after surgery needs to be accurately estimated. However, current risk stratification schemes may still have room for improvement. This study seeks to analyze prognostic factors for primary GISTs from 3 aspects, including clinicopathological parameters, immunohistochemical biomarkers, and gene mutational status, and attempts to find novel valuable factors predicting the malignancy potential of GISTs. Methods Retrospective data from 114 primary GIST patients after R0 resection were collected. Clinicopathological data was obtained from medical records and re-evaluated. Immunohistochemical analysis was performed using the Tissue Microarray method for Ki67, p16, p27, p53, SKP2, CD133, and actin. KIT gene exons 9, 11, 13, and 17 and PDGFR[alpha] gene exons 12 and 18 were tested for mutations using PCR. Results Univariate analysis revealed the following factors as poor prognostic indicators for relapse-free survival with a median follow-up of 50 months: male gender, gastrointestinal bleeding, mitotic index >5/50HPFs, tumor size >5 cm, non-gastric site, necrosis, epithelioid or mixed cell type, surrounding tissue invasion, Ki67>5%, p16>20%, p53 index >10, SKP2>10%, and KIT exon 11 deletion. Besides mitotic index, tumor size and site, SKP2 high expression (RR = 2.91, 95% CI: 1.41-5.99, P = 0.004) and KIT exon 11 deletion (RR = 2.73, 95% CI: 1.04-7.16, P = 0.041) were also independent risk factors in multivariate analysis, with gastrointestinal bleeding also showing a trend towards significance (RR = 1.88, 95% CI: 0.98-3.64, P = 0.059). In addition, gastrointestinal bleeding and SKP2 high expression showed a good ability to stratify high-risk patients further. Conclusion Our results show that gastrointestinal bleeding, SKP2 high expression, and KIT exon 11 deletions may be useful indicators of high recurrence risk for primary GIST patients.
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doi10.1371/journal.pone.0062951
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date2013-05-17