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TGF-[beta] and TGF-[beta]/Smad Signaling in the Interactions between Echinococcus multilocularis and Its Hosts.(Research Article)

Alveolar echinococcosis (AE) is characterized by the development of irreversible fibrosis and of immune tolerance towards Echinococcus multilocularis (E. multilocularis). Very little is known on the presence of transforming growth factor-[beta] (TGF-[beta]) and other components of TGF-[beta]/Smad pa... Full description

Journal Title: PLoS ONE Feb 6, 2013, Vol.8(2), p.e55379
Main Author: Wang, Junhua
Other Authors: Zhang, Chuanshan , Wei, Xufa , Blagosklonov, Oleg , Lv, Guodong , Lu, Xiaomei , Mantion, Georges , Vuitton, Dominique A. , Wen, Hao , Lin, Renyong
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 1932-6203 ; DOI: 10.1371/journal.pone.0055379
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recordid: gale_hrca478413861
title: TGF-[beta] and TGF-[beta]/Smad Signaling in the Interactions between Echinococcus multilocularis and Its Hosts.(Research Article)
format: Article
creator:
  • Wang, Junhua
  • Zhang, Chuanshan
  • Wei, Xufa
  • Blagosklonov, Oleg
  • Lv, Guodong
  • Lu, Xiaomei
  • Mantion, Georges
  • Vuitton, Dominique A.
  • Wen, Hao
  • Lin, Renyong
subjects:
  • T Cells -- Health Aspects
  • T Cells -- Analysis
  • Infection -- Health Aspects
  • Infection -- Analysis
  • Bone Morphogenetic Proteins -- Health Aspects
  • Bone Morphogenetic Proteins -- Analysis
  • Liver -- Health Aspects
  • Liver -- Analysis
  • Transforming Growth Factors -- Health Aspects
  • Transforming Growth Factors -- Analysis
  • Liver Diseases -- Health Aspects
  • Liver Diseases -- Analysis
ispartof: PLoS ONE, Feb 6, 2013, Vol.8(2), p.e55379
description: Alveolar echinococcosis (AE) is characterized by the development of irreversible fibrosis and of immune tolerance towards Echinococcus multilocularis (E. multilocularis). Very little is known on the presence of transforming growth factor-[beta] (TGF-[beta]) and other components of TGF-[beta]/Smad pathway in the liver, and on their possible influence on fibrosis, over the various stages of infection. Using Western Blot, qRT-PCR and immunohistochemistry, we measured the levels of TGF-[beta]1, TGF-[beta] receptors, and down-stream Smads activation, as well as fibrosis marker expression in both a murine AE model from day 2 to 360 post-infection (p.i.) and in AE patients. TGF-[beta]1, its receptors, and down-stream Smads were markedly expressed in the periparasitic infiltrate and also in the hepatocytes, close to and distant from AE lesions. Fibrosis was significant at 180 days p.i. in the periparasitic infiltrate and was also present in the liver parenchyma, even distant from the lesions. Over the time course after infection TGF-[beta]1 expression was correlated with CD4/CD8 T-cell ratio long described as a hallmark of AE severity. The time course of the various actors of the TGF-[beta]/Smad system in the in vivo mouse model as well as down-regulation of Smad7 in liver areas close to the lesions in human cases highly suggest that TGF-[beta] plays an important role in AE both in immune tolerance against the parasite and in liver fibrosis.
language: English
source:
identifier: ISSN: 1932-6203 ; DOI: 10.1371/journal.pone.0055379
fulltext: fulltext
issn:
  • 1932-6203
  • 19326203
url: Link


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titleTGF-[beta] and TGF-[beta]/Smad Signaling in the Interactions between Echinococcus multilocularis and Its Hosts.(Research Article)
creatorWang, Junhua ; Zhang, Chuanshan ; Wei, Xufa ; Blagosklonov, Oleg ; Lv, Guodong ; Lu, Xiaomei ; Mantion, Georges ; Vuitton, Dominique A. ; Wen, Hao ; Lin, Renyong
ispartofPLoS ONE, Feb 6, 2013, Vol.8(2), p.e55379
identifierISSN: 1932-6203 ; DOI: 10.1371/journal.pone.0055379
subjectT Cells -- Health Aspects ; T Cells -- Analysis ; Infection -- Health Aspects ; Infection -- Analysis ; Bone Morphogenetic Proteins -- Health Aspects ; Bone Morphogenetic Proteins -- Analysis ; Liver -- Health Aspects ; Liver -- Analysis ; Transforming Growth Factors -- Health Aspects ; Transforming Growth Factors -- Analysis ; Liver Diseases -- Health Aspects ; Liver Diseases -- Analysis
descriptionAlveolar echinococcosis (AE) is characterized by the development of irreversible fibrosis and of immune tolerance towards Echinococcus multilocularis (E. multilocularis). Very little is known on the presence of transforming growth factor-[beta] (TGF-[beta]) and other components of TGF-[beta]/Smad pathway in the liver, and on their possible influence on fibrosis, over the various stages of infection. Using Western Blot, qRT-PCR and immunohistochemistry, we measured the levels of TGF-[beta]1, TGF-[beta] receptors, and down-stream Smads activation, as well as fibrosis marker expression in both a murine AE model from day 2 to 360 post-infection (p.i.) and in AE patients. TGF-[beta]1, its receptors, and down-stream Smads were markedly expressed in the periparasitic infiltrate and also in the hepatocytes, close to and distant from AE lesions. Fibrosis was significant at 180 days p.i. in the periparasitic infiltrate and was also present in the liver parenchyma, even distant from the lesions. Over the time course after infection TGF-[beta]1 expression was correlated with CD4/CD8 T-cell ratio long described as a hallmark of AE severity. The time course of the various actors of the TGF-[beta]/Smad system in the in vivo mouse model as well as down-regulation of Smad7 in liver areas close to the lesions in human cases highly suggest that TGF-[beta] plays an important role in AE both in immune tolerance against the parasite and in liver fibrosis.
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9Wen, Hao
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titleTGF-[beta] and TGF-[beta]/Smad Signaling in the Interactions between Echinococcus multilocularis and Its Hosts.(Research Article)
descriptionAlveolar echinococcosis (AE) is characterized by the development of irreversible fibrosis and of immune tolerance towards Echinococcus multilocularis (E. multilocularis). Very little is known on the presence of transforming growth factor-[beta] (TGF-[beta]) and other components of TGF-[beta]/Smad pathway in the liver, and on their possible influence on fibrosis, over the various stages of infection. Using Western Blot, qRT-PCR and immunohistochemistry, we measured the levels of TGF-[beta]1, TGF-[beta] receptors, and down-stream Smads activation, as well as fibrosis marker expression in both a murine AE model from day 2 to 360 post-infection (p.i.) and in AE patients. TGF-[beta]1, its receptors, and down-stream Smads were markedly expressed in the periparasitic infiltrate and also in the hepatocytes, close to and distant from AE lesions. Fibrosis was significant at 180 days p.i. in the periparasitic infiltrate and was also present in the liver parenchyma, even distant from the lesions. Over the time course after infection TGF-[beta]1 expression was correlated with CD4/CD8 T-cell ratio long described as a hallmark of AE severity. The time course of the various actors of the TGF-[beta]/Smad system in the in vivo mouse model as well as down-regulation of Smad7 in liver areas close to the lesions in human cases highly suggest that TGF-[beta] plays an important role in AE both in immune tolerance against the parasite and in liver fibrosis.
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titleTGF-[beta] and TGF-[beta]/Smad Signaling in the Interactions between Echinococcus multilocularis and Its Hosts.(Research Article)
authorWang, Junhua ; Zhang, Chuanshan ; Wei, Xufa ; Blagosklonov, Oleg ; Lv, Guodong ; Lu, Xiaomei ; Mantion, Georges ; Vuitton, Dominique A. ; Wen, Hao ; Lin, Renyong
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abstractAlveolar echinococcosis (AE) is characterized by the development of irreversible fibrosis and of immune tolerance towards Echinococcus multilocularis (E. multilocularis). Very little is known on the presence of transforming growth factor-[beta] (TGF-[beta]) and other components of TGF-[beta]/Smad pathway in the liver, and on their possible influence on fibrosis, over the various stages of infection. Using Western Blot, qRT-PCR and immunohistochemistry, we measured the levels of TGF-[beta]1, TGF-[beta] receptors, and down-stream Smads activation, as well as fibrosis marker expression in both a murine AE model from day 2 to 360 post-infection (p.i.) and in AE patients. TGF-[beta]1, its receptors, and down-stream Smads were markedly expressed in the periparasitic infiltrate and also in the hepatocytes, close to and distant from AE lesions. Fibrosis was significant at 180 days p.i. in the periparasitic infiltrate and was also present in the liver parenchyma, even distant from the lesions. Over the time course after infection TGF-[beta]1 expression was correlated with CD4/CD8 T-cell ratio long described as a hallmark of AE severity. The time course of the various actors of the TGF-[beta]/Smad system in the in vivo mouse model as well as down-regulation of Smad7 in liver areas close to the lesions in human cases highly suggest that TGF-[beta] plays an important role in AE both in immune tolerance against the parasite and in liver fibrosis.
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date2013-02-06