schliessen

Filtern

 

Bibliotheken

Antisense for gonadotropin-releasing hormone reduces gonadotropin synthesis and gonadal development in transgenic common carp (Cyprinus carpio).(Author abstract)(Report)

To link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.aquaculture.2007.04.075 Byline: Wei Hu (a), Shuangfei Li (a), Bin Tang (a), Yaping Wang (a), Haoran Lin (b), Xiaochun Liu (b), Jun Zou (c), Zuoyan Zhu (a) Keywords: Common carp (Cyprinus carpio); Gonadotropin-... Full description

Journal Title: Aquaculture Oct 3, 2007, Vol.271(1-4), p.498(9)
Main Author: Hu, Wei
Other Authors: Li, Shuangfei , Tang, Bin , Wang, Yaping , Lin, Haoran , Liu, Xiaochun , Zou, Jun , Zhu, Zuoyan
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 0044-8486
Zum Text:
SendSend as email Add to Book BagAdd to Book Bag
Staff View
recordid: gale_ofa168296606
title: Antisense for gonadotropin-releasing hormone reduces gonadotropin synthesis and gonadal development in transgenic common carp (Cyprinus carpio).(Author abstract)(Report)
format: Article
creator:
  • Hu, Wei
  • Li, Shuangfei
  • Tang, Bin
  • Wang, Yaping
  • Lin, Haoran
  • Liu, Xiaochun
  • Zou, Jun
  • Zhu, Zuoyan
subjects:
  • Genetic Engineering
  • Microbiological Synthesis
  • Hypothalamic Hormones
ispartof: Aquaculture, Oct 3, 2007, Vol.271(1-4), p.498(9)
description: To link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.aquaculture.2007.04.075 Byline: Wei Hu (a), Shuangfei Li (a), Bin Tang (a), Yaping Wang (a), Haoran Lin (b), Xiaochun Liu (b), Jun Zou (c), Zuoyan Zhu (a) Keywords: Common carp (Cyprinus carpio); Gonadotropin-releasing hormone (GnRH); Transgene Abstract: Generating transgenic fish with desirable traits (e.g., rapid growth, larger size, etc.) for commercial use has been hampered by concerns for biosafety and competition if these fish are released into the environment. These obstacles may be overcome by producing transgenic fish that are sterile, possibly by inhibiting hormones related to reproduction. In vertebrates, synthesis and release of gonadotropin (GtH) and other reproductive hormones is mediated by gonadotropin-releasing hormone (GnRH). Recently two cDNA sequences encoding salmon-type GnRH (sGnRH) decapeptides were cloned from common carp (Cyprinus carpio). This study analyzed the expression of these two genes using real-time polymerase chain reaction (RT-PCR) in different tissues carp at varying developmental stages. Transcripts of both genes were detected in ovary and testis in mature and regressed, but not in juvenile carp. To evaluate the effects of sGnRH inhibition, the recombinant gene CAsGnRHpc-antisense, expressing antisense sGnRH RNA driven by a carp beta-actin promoter, was constructed. Blocking sGnRH expression using antisense sGnRH significantly decreased GtH in the blood of male transgenic carp. Furthermore, some antisense transgenic fish had no gonadal development and were completely sterile. These data demonstrate that sGnRH is important for GtH synthesis and development of reproductive organs in carp. Also, the antisense sGnRH strategy may prove effective in generating sterile transgenic fish, eliminating environmental concerns these fish may raise. Author Affiliation: (a) State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China (b) College of Life Sciences, Zhongshan University, Guangzhou, 510275, China (c) School of Biological Sciences, University of Aberdeen, Aberdeen AB24 2TZ, UK Article History: Received 5 May 2006; Revised 25 April 2007; Accepted 25 April 2007
language: English
source:
identifier: ISSN: 0044-8486
fulltext: fulltext
issn:
  • 0044-8486
  • 00448486
url: Link


@attributes
ID561457257
RANK0.06999999
NO1
SEARCH_ENGINEprimo_central_multiple_fe
SEARCH_ENGINE_TYPEPrimo Central Search Engine
LOCALfalse
PrimoNMBib
record
control
sourcerecordid168296606
sourceidgale_ofa
recordidTN_gale_ofa168296606
sourceformatXML
sourcesystemPC
pqid14822175
galeid168296606
display
typearticle
titleAntisense for gonadotropin-releasing hormone reduces gonadotropin synthesis and gonadal development in transgenic common carp (Cyprinus carpio).(Author abstract)(Report)
creatorHu, Wei ; Li, Shuangfei ; Tang, Bin ; Wang, Yaping ; Lin, Haoran ; Liu, Xiaochun ; Zou, Jun ; Zhu, Zuoyan
ispartofAquaculture, Oct 3, 2007, Vol.271(1-4), p.498(9)
identifierISSN: 0044-8486
subjectGenetic Engineering ; Microbiological Synthesis ; Hypothalamic Hormones
descriptionTo link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.aquaculture.2007.04.075 Byline: Wei Hu (a), Shuangfei Li (a), Bin Tang (a), Yaping Wang (a), Haoran Lin (b), Xiaochun Liu (b), Jun Zou (c), Zuoyan Zhu (a) Keywords: Common carp (Cyprinus carpio); Gonadotropin-releasing hormone (GnRH); Transgene Abstract: Generating transgenic fish with desirable traits (e.g., rapid growth, larger size, etc.) for commercial use has been hampered by concerns for biosafety and competition if these fish are released into the environment. These obstacles may be overcome by producing transgenic fish that are sterile, possibly by inhibiting hormones related to reproduction. In vertebrates, synthesis and release of gonadotropin (GtH) and other reproductive hormones is mediated by gonadotropin-releasing hormone (GnRH). Recently two cDNA sequences encoding salmon-type GnRH (sGnRH) decapeptides were cloned from common carp (Cyprinus carpio). This study analyzed the expression of these two genes using real-time polymerase chain reaction (RT-PCR) in different tissues carp at varying developmental stages. Transcripts of both genes were detected in ovary and testis in mature and regressed, but not in juvenile carp. To evaluate the effects of sGnRH inhibition, the recombinant gene CAsGnRHpc-antisense, expressing antisense sGnRH RNA driven by a carp beta-actin promoter, was constructed. Blocking sGnRH expression using antisense sGnRH significantly decreased GtH in the blood of male transgenic carp. Furthermore, some antisense transgenic fish had no gonadal development and were completely sterile. These data demonstrate that sGnRH is important for GtH synthesis and development of reproductive organs in carp. Also, the antisense sGnRH strategy may prove effective in generating sterile transgenic fish, eliminating environmental concerns these fish may raise. Author Affiliation: (a) State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China (b) College of Life Sciences, Zhongshan University, Guangzhou, 510275, China (c) School of Biological Sciences, University of Aberdeen, Aberdeen AB24 2TZ, UK Article History: Received 5 May 2006; Revised 25 April 2007; Accepted 25 April 2007
languageEnglish
source
version6
lds50peer_reviewed
links
openurl$$Topenurl_article
openurlfulltext$$Topenurlfull_article
search
scope
0gale_onefilea
1OneFile
creatorcontrib
0Hu, Wei
1Wei Hu
2Li, Shuangfei
3Tang, Bin
4Wang, Yaping
5Lin, Haoran
6Liu, Xiaochun
7Zou, Jun
8Zhu, Zuoyan
titleAntisense for gonadotropin-releasing hormone reduces gonadotropin synthesis and gonadal development in transgenic common carp (Cyprinus carpio).(Author abstract)(Report)
descriptionTo link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.aquaculture.2007.04.075 Byline: Wei Hu (a), Shuangfei Li (a), Bin Tang (a), Yaping Wang (a), Haoran Lin (b), Xiaochun Liu (b), Jun Zou (c), Zuoyan Zhu (a) Keywords: Common carp (Cyprinus carpio); Gonadotropin-releasing hormone (GnRH); Transgene Abstract: Generating transgenic fish with desirable traits (e.g., rapid growth, larger size, etc.) for commercial use has been hampered by concerns for biosafety and competition if these fish are released into the environment. These obstacles may be overcome by producing transgenic fish that are sterile, possibly by inhibiting hormones related to reproduction. In vertebrates, synthesis and release of gonadotropin (GtH) and other reproductive hormones is mediated by gonadotropin-releasing hormone (GnRH). Recently two cDNA sequences encoding salmon-type GnRH (sGnRH) decapeptides were cloned from common carp (Cyprinus carpio). This study analyzed the expression of these two genes using real-time polymerase chain reaction (RT-PCR) in different tissues carp at varying developmental stages. Transcripts of both genes were detected in ovary and testis in mature and regressed, but not in juvenile carp. To evaluate the effects of sGnRH inhibition, the recombinant gene CAsGnRHpc-antisense, expressing antisense sGnRH RNA driven by a carp beta-actin promoter, was constructed. Blocking sGnRH expression using antisense sGnRH significantly decreased GtH in the blood of male transgenic carp. Furthermore, some antisense transgenic fish had no gonadal development and were completely sterile. These data demonstrate that sGnRH is important for GtH synthesis and development of reproductive organs in carp. Also, the antisense sGnRH strategy may prove effective in generating sterile transgenic fish, eliminating environmental concerns these fish may raise. Author Affiliation: (a) State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China (b) College of Life Sciences, Zhongshan University, Guangzhou, 510275, China (c) School of Biological Sciences, University of Aberdeen, Aberdeen AB24 2TZ, UK Article History: Received 5 May 2006; Revised 25 April 2007; Accepted 25 April 2007
subject
0Genetic engineering
1Microbiological synthesis
2Hypothalamic hormones
3Research and Development in the Physical, Engineering, and Life Sciences
454171
general
0English
1Elsevier B.V.
2Cengage Learning, Inc.
sourceidgale_ofa
recordidgale_ofa168296606
issn
00044-8486
100448486
rsrctypearticle
creationdate2007
recordtypearticle
addtitleAquaculture
searchscopeOneFile
lsr30VSR-Enriched:[pages, eissn, galeid, doi, pqid]
sort
titleAntisense for gonadotropin-releasing hormone reduces gonadotropin synthesis and gonadal development in transgenic common carp (Cyprinus carpio).(Author abstract)(Report)
authorHu, Wei ; Li, Shuangfei ; Tang, Bin ; Wang, Yaping ; Lin, Haoran ; Liu, Xiaochun ; Zou, Jun ; Zhu, Zuoyan
creationdate20071003
facets
frbrgroupid5178599329243559293
frbrtype5
languageeng
creationdate2007
topic
0Genetic Engineering
1Microbiological Synthesis
2Hypothalamic Hormones
collectionOneFile (GALE)
prefilterarticles
rsrctypearticles
creatorcontrib
0Hu, Wei
1Li, Shuangfei
2Tang, Bin
3Wang, Yaping
4Lin, Haoran
5Liu, Xiaochun
6Zou, Jun
7Zhu, Zuoyan
jtitleAquaculture
toplevelpeer_reviewed
frbr
t2
k12007
k200448486
k4271
k51
k6498
k7aquaculture
k8antisense for gonadotropin releasing hormone reduces gonadotropin synthesis gonadal development in transgenic common carp
k9antisenseforgonadotrncarp
k12antisenseforgonadotropinr
k15weihu
k16huwei
delivery
delcategoryRemote Search Resource
fulltextfulltext
ranking
booster11
booster21
pcg_typeaggregator
addata
au
0Hu, Wei
1Li, Shuangfei
2Tang, Bin
3Wang, Yaping
4Lin, Haoran
5Liu, Xiaochun
6Zou, Jun
7Zhu, Zuoyan
atitleAntisense for gonadotropin-releasing hormone reduces gonadotropin synthesis and gonadal development in transgenic common carp (Cyprinus carpio).(Author abstract)(Report)
jtitleAquaculture
risdate20071003
volume271
issue1 4
spage498
issn0044-8486
genrearticle
ristypeJOUR
abstractTo link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.aquaculture.2007.04.075 Byline: Wei Hu (a), Shuangfei Li (a), Bin Tang (a), Yaping Wang (a), Haoran Lin (b), Xiaochun Liu (b), Jun Zou (c), Zuoyan Zhu (a) Keywords: Common carp (Cyprinus carpio); Gonadotropin-releasing hormone (GnRH); Transgene Abstract: Generating transgenic fish with desirable traits (e.g., rapid growth, larger size, etc.) for commercial use has been hampered by concerns for biosafety and competition if these fish are released into the environment. These obstacles may be overcome by producing transgenic fish that are sterile, possibly by inhibiting hormones related to reproduction. In vertebrates, synthesis and release of gonadotropin (GtH) and other reproductive hormones is mediated by gonadotropin-releasing hormone (GnRH). Recently two cDNA sequences encoding salmon-type GnRH (sGnRH) decapeptides were cloned from common carp (Cyprinus carpio). This study analyzed the expression of these two genes using real-time polymerase chain reaction (RT-PCR) in different tissues carp at varying developmental stages. Transcripts of both genes were detected in ovary and testis in mature and regressed, but not in juvenile carp. To evaluate the effects of sGnRH inhibition, the recombinant gene CAsGnRHpc-antisense, expressing antisense sGnRH RNA driven by a carp beta-actin promoter, was constructed. Blocking sGnRH expression using antisense sGnRH significantly decreased GtH in the blood of male transgenic carp. Furthermore, some antisense transgenic fish had no gonadal development and were completely sterile. These data demonstrate that sGnRH is important for GtH synthesis and development of reproductive organs in carp. Also, the antisense sGnRH strategy may prove effective in generating sterile transgenic fish, eliminating environmental concerns these fish may raise. Author Affiliation: (a) State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China (b) College of Life Sciences, Zhongshan University, Guangzhou, 510275, China (c) School of Biological Sciences, University of Aberdeen, Aberdeen AB24 2TZ, UK Article History: Received 5 May 2006; Revised 25 April 2007; Accepted 25 April 2007
pubElsevier B.V.
lad01gale_ofa
pages498-506
doi10.1016/j.aquaculture.2007.04.075
eissn18735622
date2007-10-03