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Continuous 24-h L-[1-13C]phenylalanine and L-[3,3-2H2]tyrosine oral-tracer studies at an intermediate phenylalanine intake to estimate requirements in adults

The daily rates of whole-body phenylalanine oxidation (pine-ox) and hydroxylation (pine-OH) were determined in young men (n = 10) receiving [[sup.13.C]phenylalanine and [[sup.2.][H.sub.2]]tyrosine via primed constant oral infusion (four also received simultaneously [[2.sup.][H.sub.4]]tyrosine and [[... Full description

Journal Title: American Journal of Clinical Nutrition Feb, 1997, Vol.65(2), p.473(16)
Main Author: Basile - Filho, Anibal
Other Authors: El - Khoury, Antoine E. , Beaumier, Louis , Wang, San Y. , Young, Vernon R.
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ID: ISSN: 0002-9165
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title: Continuous 24-h L-[1-13C]phenylalanine and L-[3,3-2H2]tyrosine oral-tracer studies at an intermediate phenylalanine intake to estimate requirements in adults
format: Article
creator:
  • Basile - Filho, Anibal
  • El - Khoury, Antoine E.
  • Beaumier, Louis
  • Wang, San Y.
  • Young, Vernon R.
subjects:
  • Amino Acid Metabolism -- Health Aspects
  • Phenylalanine -- Physiological Aspects
  • Nutritional Requirements
ispartof: American Journal of Clinical Nutrition, Feb, 1997, Vol.65(2), p.473(16)
description: The daily rates of whole-body phenylalanine oxidation (pine-ox) and hydroxylation (pine-OH) were determined in young men (n = 10) receiving [[sup.13.C]phenylalanine and [[sup.2.][H.sub.2]]tyrosine via primed constant oral infusion (four also received simultaneously [[2.sup.][H.sub.4]]tyrosine and [[2.sup.][H.sub.3]]leucine via primed constant intravenous infusions) continuously for 24 h (first 12 h fast and then 12 h fed). The subjects were given a diet supplying a proposed requirement phenylalanine intake (six subjects: 39 mg phenylalanine [multiplied by] [kg.sup. -1] [multiplied by] [d.sup. -1] without tyrosine; four subjects: 36 mg phenylalanine plus 6.8 mg tyrosine), based on an otherwise adequate L-amino acid mixture for 6 d before the tracer study. Our hypothesis was that the subjects would be in approximate body phenylalanine equilibrium at these intakes. Estimates of the daily rate of pine-ox were 26.9 [+ or -] 7.5 mg [multiplied by] [kg.sup -1] [multiplied by] [d.sup. -1] (17.2 [+ or -] 5.2 and 9.7 [+ or -] 3.2 mg [multiplied by] [kg.sup. -1] [multiplied by] [d.sup. -1] during the 12-h fast and fed periods, respectively), and for pine-OH they were 32.1 [+ or -] 11.9 mg [multiplied by] [kg.sup. -1] [multiplied by] [d.sup. -1] (21.7 [+ or -] 10.5 and 10.4 [+ or -] 2.5 mg [multiplied by] [kg.sup. -1] [multiplied by] [d.sup. -1] during the 12-h fast and fed periods, respectively). The daily phenylalanine balance was approximately neutral (P [is greater than] 0.05) when based on pine-ox or pine-OH (+4.73 [+ or -] 7.34 and -0.41 [+ or -] 12.6 mg [multiplied by] [kg.sup. -1] [multiplied by] [d.sup. -1], respectively). In comparison with recent, comparable 24-h tracer studies at deficient (22 mg [multiplied by] [kg.sup. -1] [multiplied by] [d.sup. -1]) and generous (100 mg [multiplied by] [kg.sup. -1] [multiplied by] [d.sup. -1]) phenylalanine intakes, these results support the hypothesis that a phenylalanine intake of 39 mg [multiplied by] [kg.sup. -1] [multiplied by] [d.sup. -1] (without significant tyrosine) approximates the mean requirement in healthy adults. This contrasts with the upper requirement value of 14 mg [multiplied by] [kg.sup. -1] [multiplied by] [d.sup. -1] for the total of the aromatic amino acids proposed in 1985 by FAO/WHO/UNU. Am J Clin Nutr 1997;65:473-88.
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identifier: ISSN: 0002-9165
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  • 0002-9165
  • 00029165
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titleContinuous 24-h L-[1-13C]phenylalanine and L-[3,3-2H2]tyrosine oral-tracer studies at an intermediate phenylalanine intake to estimate requirements in adults
creatorBasile - Filho, Anibal ; El - Khoury, Antoine E. ; Beaumier, Louis ; Wang, San Y. ; Young, Vernon R.
ispartofAmerican Journal of Clinical Nutrition, Feb, 1997, Vol.65(2), p.473(16)
identifierISSN: 0002-9165
subjectAmino Acid Metabolism -- Health Aspects ; Phenylalanine -- Physiological Aspects ; Nutritional Requirements
descriptionThe daily rates of whole-body phenylalanine oxidation (pine-ox) and hydroxylation (pine-OH) were determined in young men (n = 10) receiving [[sup.13.C]phenylalanine and [[sup.2.][H.sub.2]]tyrosine via primed constant oral infusion (four also received simultaneously [[2.sup.][H.sub.4]]tyrosine and [[2.sup.][H.sub.3]]leucine via primed constant intravenous infusions) continuously for 24 h (first 12 h fast and then 12 h fed). The subjects were given a diet supplying a proposed requirement phenylalanine intake (six subjects: 39 mg phenylalanine [multiplied by] [kg.sup. -1] [multiplied by] [d.sup. -1] without tyrosine; four subjects: 36 mg phenylalanine plus 6.8 mg tyrosine), based on an otherwise adequate L-amino acid mixture for 6 d before the tracer study. Our hypothesis was that the subjects would be in approximate body phenylalanine equilibrium at these intakes. Estimates of the daily rate of pine-ox were 26.9 [+ or -] 7.5 mg [multiplied by] [kg.sup -1] [multiplied by] [d.sup. -1] (17.2 [+ or -] 5.2 and 9.7 [+ or -] 3.2 mg [multiplied by] [kg.sup. -1] [multiplied by] [d.sup. -1] during the 12-h fast and fed periods, respectively), and for pine-OH they were 32.1 [+ or -] 11.9 mg [multiplied by] [kg.sup. -1] [multiplied by] [d.sup. -1] (21.7 [+ or -] 10.5 and 10.4 [+ or -] 2.5 mg [multiplied by] [kg.sup. -1] [multiplied by] [d.sup. -1] during the 12-h fast and fed periods, respectively). The daily phenylalanine balance was approximately neutral (P [is greater than] 0.05) when based on pine-ox or pine-OH (+4.73 [+ or -] 7.34 and -0.41 [+ or -] 12.6 mg [multiplied by] [kg.sup. -1] [multiplied by] [d.sup. -1], respectively). In comparison with recent, comparable 24-h tracer studies at deficient (22 mg [multiplied by] [kg.sup. -1] [multiplied by] [d.sup. -1]) and generous (100 mg [multiplied by] [kg.sup. -1] [multiplied by] [d.sup. -1]) phenylalanine intakes, these results support the hypothesis that a phenylalanine intake of 39 mg [multiplied by] [kg.sup. -1] [multiplied by] [d.sup. -1] (without significant tyrosine) approximates the mean requirement in healthy adults. This contrasts with the upper requirement value of 14 mg [multiplied by] [kg.sup. -1] [multiplied by] [d.sup. -1] for the total of the aromatic amino acids proposed in 1985 by FAO/WHO/UNU. Am J Clin Nutr 1997;65:473-88.
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titleContinuous 24-h L-[1-13C]phenylalanine and L-[3,3-2H2]tyrosine oral-tracer studies at an intermediate phenylalanine intake to estimate requirements in adults.
descriptionThe daily rates of whole-body phenylalanine oxidation (pine-ox) and hydroxylation (pine-OH) were determined in young men (n = 10) receiving [[sup.13.C]phenylalanine and [[sup.2.][H.sub.2]]tyrosine via primed constant oral infusion (four also received simultaneously [[2.sup.][H.sub.4]]tyrosine and [[2.sup.][H.sub.3]]leucine via primed constant intravenous infusions) continuously for 24 h (first 12 h fast and then 12 h fed). The subjects were given a diet supplying a proposed requirement phenylalanine intake (six subjects: 39 mg phenylalanine [multiplied by] [kg.sup. -1] [multiplied by] [d.sup. -1] without tyrosine; four subjects: 36 mg phenylalanine plus 6.8 mg tyrosine), based on an otherwise adequate L-amino acid mixture for 6 d before the tracer study. Our hypothesis was that the subjects would be in approximate body phenylalanine equilibrium at these intakes. Estimates of the daily rate of pine-ox were 26.9 [+ or -] 7.5 mg [multiplied by] [kg.sup -1] [multiplied by] [d.sup. -1] (17.2 [+ or -] 5.2 and 9.7 [+ or -] 3.2 mg [multiplied by] [kg.sup. -1] [multiplied by] [d.sup. -1] during the 12-h fast and fed periods, respectively), and for pine-OH they were 32.1 [+ or -] 11.9 mg [multiplied by] [kg.sup. -1] [multiplied by] [d.sup. -1] (21.7 [+ or -] 10.5 and 10.4 [+ or -] 2.5 mg [multiplied by] [kg.sup. -1] [multiplied by] [d.sup. -1] during the 12-h fast and fed periods, respectively). The daily phenylalanine balance was approximately neutral (P [is greater than] 0.05) when based on pine-ox or pine-OH (+4.73 [+ or -] 7.34 and -0.41 [+ or -] 12.6 mg [multiplied by] [kg.sup. -1] [multiplied by] [d.sup. -1], respectively). In comparison with recent, comparable 24-h tracer studies at deficient (22 mg [multiplied by] [kg.sup. -1] [multiplied by] [d.sup. -1]) and generous (100 mg [multiplied by] [kg.sup. -1] [multiplied by] [d.sup. -1]) phenylalanine intakes, these results support the hypothesis that a phenylalanine intake of 39 mg [multiplied by] [kg.sup. -1] [multiplied by] [d.sup. -1] (without significant tyrosine) approximates the mean requirement in healthy adults. This contrasts with the upper requirement value of 14 mg [multiplied by] [kg.sup. -1] [multiplied by] [d.sup. -1] for the total of the aromatic amino acids proposed in 1985 by FAO/WHO/UNU. Am J Clin Nutr 1997;65:473-88.
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abstractThe daily rates of whole-body phenylalanine oxidation (pine-ox) and hydroxylation (pine-OH) were determined in young men (n = 10) receiving [[sup.13.C]phenylalanine and [[sup.2.][H.sub.2]]tyrosine via primed constant oral infusion (four also received simultaneously [[2.sup.][H.sub.4]]tyrosine and [[2.sup.][H.sub.3]]leucine via primed constant intravenous infusions) continuously for 24 h (first 12 h fast and then 12 h fed). The subjects were given a diet supplying a proposed requirement phenylalanine intake (six subjects: 39 mg phenylalanine [multiplied by] [kg.sup. -1] [multiplied by] [d.sup. -1] without tyrosine; four subjects: 36 mg phenylalanine plus 6.8 mg tyrosine), based on an otherwise adequate L-amino acid mixture for 6 d before the tracer study. Our hypothesis was that the subjects would be in approximate body phenylalanine equilibrium at these intakes. Estimates of the daily rate of pine-ox were 26.9 [+ or -] 7.5 mg [multiplied by] [kg.sup -1] [multiplied by] [d.sup. -1] (17.2 [+ or -] 5.2 and 9.7 [+ or -] 3.2 mg [multiplied by] [kg.sup. -1] [multiplied by] [d.sup. -1] during the 12-h fast and fed periods, respectively), and for pine-OH they were 32.1 [+ or -] 11.9 mg [multiplied by] [kg.sup. -1] [multiplied by] [d.sup. -1] (21.7 [+ or -] 10.5 and 10.4 [+ or -] 2.5 mg [multiplied by] [kg.sup. -1] [multiplied by] [d.sup. -1] during the 12-h fast and fed periods, respectively). The daily phenylalanine balance was approximately neutral (P [is greater than] 0.05) when based on pine-ox or pine-OH (+4.73 [+ or -] 7.34 and -0.41 [+ or -] 12.6 mg [multiplied by] [kg.sup. -1] [multiplied by] [d.sup. -1], respectively). In comparison with recent, comparable 24-h tracer studies at deficient (22 mg [multiplied by] [kg.sup. -1] [multiplied by] [d.sup. -1]) and generous (100 mg [multiplied by] [kg.sup. -1] [multiplied by] [d.sup. -1]) phenylalanine intakes, these results support the hypothesis that a phenylalanine intake of 39 mg [multiplied by] [kg.sup. -1] [multiplied by] [d.sup. -1] (without significant tyrosine) approximates the mean requirement in healthy adults. This contrasts with the upper requirement value of 14 mg [multiplied by] [kg.sup. -1] [multiplied by] [d.sup. -1] for the total of the aromatic amino acids proposed in 1985 by FAO/WHO/UNU. Am J Clin Nutr 1997;65:473-88.
pubAmerican Society for Clinical Nutrition, Inc.
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doi10.1093/ajcn/65.2.473
pages473-47388
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date1997-02-01