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A critical domain of the Cucumber mosaic virus 2b protein for RNA silencing suppressor activity.(Report)

To link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.febslet.2008.11.031 Byline: Jian Ye (a)(b)(c), Jing Qu (a)(b)(c), Jian-Feng Zhang (a)(b), Yun-Feng Geng (a)(b)(c), Rong-Xiang Fang (a)(b) Keywords: Viral RNA silencing suppressor; Argonaute4; Cucumber mosaic v... Full description

Journal Title: FEBS Letters Jan 5, 2009, Vol.583(1), p.101(6)
Main Author: Ye, Jian
Other Authors: Qu, Jing , Zhang, Jian - Feng , Geng, Yun - Feng , Fang, Rong - Xiang
Format: Electronic Article Electronic Article
Language: English
Subjects:
Rna
ID: ISSN: 0014-5793
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recordid: gale_ofa202133236
title: A critical domain of the Cucumber mosaic virus 2b protein for RNA silencing suppressor activity.(Report)
format: Article
creator:
  • Ye, Jian
  • Qu, Jing
  • Zhang, Jian - Feng
  • Geng, Yun - Feng
  • Fang, Rong - Xiang
subjects:
  • Proteins
  • Methylation
  • Rna
  • Plant Genetics
  • Virus Diseases
ispartof: FEBS Letters, Jan 5, 2009, Vol.583(1), p.101(6)
description: To link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.febslet.2008.11.031 Byline: Jian Ye (a)(b)(c), Jing Qu (a)(b)(c), Jian-Feng Zhang (a)(b), Yun-Feng Geng (a)(b)(c), Rong-Xiang Fang (a)(b) Keywords: Viral RNA silencing suppressor; Argonaute4; Cucumber mosaic virus Abstract: Alignment of Cucumber mosaic virus (CMV) 2b protein sequences from two CMV subgroups revealed two highly variable regions. To examine contributions of variable sequence domains to the suppressor activity, we performed a comparative study between 2b proteins of a subgroup I strain (SD-CMV) and a subgroup II strain (Q-CMV). Here we show that the suppressor activity of SD2b is stronger than that of Q2b and that a domain existent in SD2b but absent in Q2b is a major determinant of the suppressor activity of SD2b. We further show that the same domain is responsible for inhibition of Nicotiana benthamiana AGO4-1 transcription. Our results implicate AGO4 as a mediator for CMV 2b to suppress systemic silencing and DNA methylation. Author Affiliation: (a) State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, A3 Datun Road, Chaoyang District, Beijing 100101, China (b) National Plant Gene Research Center, Beijing 100101, China (c) Graduate School of the Chinese Academy of Sciences, Beijing 100049, China Article History: Received 5 August 2008; Revised 10 November 2008; Accepted 19 November 2008
language: English
source:
identifier: ISSN: 0014-5793
fulltext: fulltext
issn:
  • 0014-5793
  • 00145793
url: Link


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titleA critical domain of the Cucumber mosaic virus 2b protein for RNA silencing suppressor activity.(Report)
creatorYe, Jian ; Qu, Jing ; Zhang, Jian - Feng ; Geng, Yun - Feng ; Fang, Rong - Xiang
ispartofFEBS Letters, Jan 5, 2009, Vol.583(1), p.101(6)
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subjectProteins ; Methylation ; Rna ; Plant Genetics ; Virus Diseases
descriptionTo link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.febslet.2008.11.031 Byline: Jian Ye (a)(b)(c), Jing Qu (a)(b)(c), Jian-Feng Zhang (a)(b), Yun-Feng Geng (a)(b)(c), Rong-Xiang Fang (a)(b) Keywords: Viral RNA silencing suppressor; Argonaute4; Cucumber mosaic virus Abstract: Alignment of Cucumber mosaic virus (CMV) 2b protein sequences from two CMV subgroups revealed two highly variable regions. To examine contributions of variable sequence domains to the suppressor activity, we performed a comparative study between 2b proteins of a subgroup I strain (SD-CMV) and a subgroup II strain (Q-CMV). Here we show that the suppressor activity of SD2b is stronger than that of Q2b and that a domain existent in SD2b but absent in Q2b is a major determinant of the suppressor activity of SD2b. We further show that the same domain is responsible for inhibition of Nicotiana benthamiana AGO4-1 transcription. Our results implicate AGO4 as a mediator for CMV 2b to suppress systemic silencing and DNA methylation. Author Affiliation: (a) State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, A3 Datun Road, Chaoyang District, Beijing 100101, China (b) National Plant Gene Research Center, Beijing 100101, China (c) Graduate School of the Chinese Academy of Sciences, Beijing 100049, China Article History: Received 5 August 2008; Revised 10 November 2008; Accepted 19 November 2008
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descriptionTo link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.febslet.2008.11.031 Byline: Jian Ye (a)(b)(c), Jing Qu (a)(b)(c), Jian-Feng Zhang (a)(b), Yun-Feng Geng (a)(b)(c), Rong-Xiang Fang (a)(b) Keywords: Viral RNA silencing suppressor; Argonaute4; Cucumber mosaic virus Abstract: Alignment of Cucumber mosaic virus (CMV) 2b protein sequences from two CMV subgroups revealed two highly variable regions. To examine contributions of variable sequence domains to the suppressor activity, we performed a comparative study between 2b proteins of a subgroup I strain (SD-CMV) and a subgroup II strain (Q-CMV). Here we show that the suppressor activity of SD2b is stronger than that of Q2b and that a domain existent in SD2b but absent in Q2b is a major determinant of the suppressor activity of SD2b. We further show that the same domain is responsible for inhibition of Nicotiana benthamiana AGO4-1 transcription. Our results implicate AGO4 as a mediator for CMV 2b to suppress systemic silencing and DNA methylation. Author Affiliation: (a) State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, A3 Datun Road, Chaoyang District, Beijing 100101, China (b) National Plant Gene Research Center, Beijing 100101, China (c) Graduate School of the Chinese Academy of Sciences, Beijing 100049, China Article History: Received 5 August 2008; Revised 10 November 2008; Accepted 19 November 2008
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abstractTo link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.febslet.2008.11.031 Byline: Jian Ye (a)(b)(c), Jing Qu (a)(b)(c), Jian-Feng Zhang (a)(b), Yun-Feng Geng (a)(b)(c), Rong-Xiang Fang (a)(b) Keywords: Viral RNA silencing suppressor; Argonaute4; Cucumber mosaic virus Abstract: Alignment of Cucumber mosaic virus (CMV) 2b protein sequences from two CMV subgroups revealed two highly variable regions. To examine contributions of variable sequence domains to the suppressor activity, we performed a comparative study between 2b proteins of a subgroup I strain (SD-CMV) and a subgroup II strain (Q-CMV). Here we show that the suppressor activity of SD2b is stronger than that of Q2b and that a domain existent in SD2b but absent in Q2b is a major determinant of the suppressor activity of SD2b. We further show that the same domain is responsible for inhibition of Nicotiana benthamiana AGO4-1 transcription. Our results implicate AGO4 as a mediator for CMV 2b to suppress systemic silencing and DNA methylation. Author Affiliation: (a) State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, A3 Datun Road, Chaoyang District, Beijing 100101, China (b) National Plant Gene Research Center, Beijing 100101, China (c) Graduate School of the Chinese Academy of Sciences, Beijing 100049, China Article History: Received 5 August 2008; Revised 10 November 2008; Accepted 19 November 2008
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