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VirA: A virulence-related gene of Streptococcus suis serotype 2.(Report)

To link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.micpath.2010.07.001 Byline: Peng Li (a)(b)(c), Jun Liu (b)(c), Lingwei Zhu (b)(c), Chong Qi (b)(c), Weicheng Bei (d), Xuehui Cai (e), Yang Sun (b)(c), Shuzhang Feng (b)(c) Abstract: Streptococcus suis serotype... Full description

Journal Title: Microbial Pathogenesis Nov, 2010, Vol.49(5), p.305(6)
Main Author: Li, Peng
Other Authors: Liu, Jun , Zhu, Lingwei , Qi, Chong , Bei, Weicheng , Cai, Xuehui , Sun, Yang , Feng, Shuzhang
Format: Electronic Article Electronic Article
Language: English
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ID: ISSN: 0882-4010
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title: VirA: A virulence-related gene of Streptococcus suis serotype 2.(Report)
format: Article
creator:
  • Li, Peng
  • Liu, Jun
  • Zhu, Lingwei
  • Qi, Chong
  • Bei, Weicheng
  • Cai, Xuehui
  • Sun, Yang
  • Feng, Shuzhang
subjects:
  • Bacterial Genetics -- Analysis
  • Bacterial Genetics -- Health Aspects
ispartof: Microbial Pathogenesis, Nov, 2010, Vol.49(5), p.305(6)
description: To link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.micpath.2010.07.001 Byline: Peng Li (a)(b)(c), Jun Liu (b)(c), Lingwei Zhu (b)(c), Chong Qi (b)(c), Weicheng Bei (d), Xuehui Cai (e), Yang Sun (b)(c), Shuzhang Feng (b)(c) Abstract: Streptococcus suis serotype 2 is an important porcine and human zoonotic pathogen. Few virulence factors have been identified and the pathogenesis of infection is not fully understood. We have identified a novel virulence-related gene, virA, of S. suis 2. To investigate its role in pathogenesis, a virA deletion mutant of S. suis 2 wild-type strain ZY458, 458[DELTA]vir, was constructed using suicide vector pSET4s, and a functionally complemented strain 458[DELTA]vir(pvir) was constructed using shuttle plasmid pAT18 containing the virA gene. All rabbits infected with ZY458 and 458[DELTA]vir(pvir) exhibited body weight loss and developed severe clinical symptoms. All 5 rabbits infected with ZY458, and 4 of 5 infected with 458[DELTA]vir(pvir), died within 6 days of infection. In contrast, all 5 rabbits inoculated with 458[DELTA]vir gained weight normally and none developed any clinical symptoms during the entire course monitored. These results indicate that virA plays an important role in the pathogenicity of S. suis serotype 2. To investigate the virA gene further, we compared and analyzed the sequences of virA from wild-type virulent and avirulent strains. Results showed that functional virA appears to exist only in virulent strains and has been structurally mutated in avirulent strains. In conclusion, virA gene is a novel identified virulence-relate gene. It may play a key role in the evolution and pathogenicity of S. suis serotype 2. Author Affiliation: (a) College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, PR China (b) Institute of Veterinary Science, The Academy of Military Medical Sciences, Changchun 130062, PR China (c) Key laboratory of Jilin province for zoonosis prevention and control, Changchun 130062, PR China (d) State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, PR China (e) Harbin Veterinary Institute, Chinese Academy of Agriculture Sciences, Harbin 150001, PR China Article History: Received 7 December 2009; Revised 30 June 2010; Accepted 3 July 2010
language: English
source:
identifier: ISSN: 0882-4010
fulltext: fulltext
issn:
  • 0882-4010
  • 08824010
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titleVirA: A virulence-related gene of Streptococcus suis serotype 2.(Report)
creatorLi, Peng ; Liu, Jun ; Zhu, Lingwei ; Qi, Chong ; Bei, Weicheng ; Cai, Xuehui ; Sun, Yang ; Feng, Shuzhang
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descriptionTo link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.micpath.2010.07.001 Byline: Peng Li (a)(b)(c), Jun Liu (b)(c), Lingwei Zhu (b)(c), Chong Qi (b)(c), Weicheng Bei (d), Xuehui Cai (e), Yang Sun (b)(c), Shuzhang Feng (b)(c) Abstract: Streptococcus suis serotype 2 is an important porcine and human zoonotic pathogen. Few virulence factors have been identified and the pathogenesis of infection is not fully understood. We have identified a novel virulence-related gene, virA, of S. suis 2. To investigate its role in pathogenesis, a virA deletion mutant of S. suis 2 wild-type strain ZY458, 458[DELTA]vir, was constructed using suicide vector pSET4s, and a functionally complemented strain 458[DELTA]vir(pvir) was constructed using shuttle plasmid pAT18 containing the virA gene. All rabbits infected with ZY458 and 458[DELTA]vir(pvir) exhibited body weight loss and developed severe clinical symptoms. All 5 rabbits infected with ZY458, and 4 of 5 infected with 458[DELTA]vir(pvir), died within 6 days of infection. In contrast, all 5 rabbits inoculated with 458[DELTA]vir gained weight normally and none developed any clinical symptoms during the entire course monitored. These results indicate that virA plays an important role in the pathogenicity of S. suis serotype 2. To investigate the virA gene further, we compared and analyzed the sequences of virA from wild-type virulent and avirulent strains. Results showed that functional virA appears to exist only in virulent strains and has been structurally mutated in avirulent strains. In conclusion, virA gene is a novel identified virulence-relate gene. It may play a key role in the evolution and pathogenicity of S. suis serotype 2. Author Affiliation: (a) College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, PR China (b) Institute of Veterinary Science, The Academy of Military Medical Sciences, Changchun 130062, PR China (c) Key laboratory of Jilin province for zoonosis prevention and control, Changchun 130062, PR China (d) State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, PR China (e) Harbin Veterinary Institute, Chinese Academy of Agriculture Sciences, Harbin 150001, PR China Article History: Received 7 December 2009; Revised 30 June 2010; Accepted 3 July 2010
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titleVirA: A virulence-related gene of Streptococcus suis serotype 2.(Report)
descriptionTo link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.micpath.2010.07.001 Byline: Peng Li (a)(b)(c), Jun Liu (b)(c), Lingwei Zhu (b)(c), Chong Qi (b)(c), Weicheng Bei (d), Xuehui Cai (e), Yang Sun (b)(c), Shuzhang Feng (b)(c) Abstract: Streptococcus suis serotype 2 is an important porcine and human zoonotic pathogen. Few virulence factors have been identified and the pathogenesis of infection is not fully understood. We have identified a novel virulence-related gene, virA, of S. suis 2. To investigate its role in pathogenesis, a virA deletion mutant of S. suis 2 wild-type strain ZY458, 458[DELTA]vir, was constructed using suicide vector pSET4s, and a functionally complemented strain 458[DELTA]vir(pvir) was constructed using shuttle plasmid pAT18 containing the virA gene. All rabbits infected with ZY458 and 458[DELTA]vir(pvir) exhibited body weight loss and developed severe clinical symptoms. All 5 rabbits infected with ZY458, and 4 of 5 infected with 458[DELTA]vir(pvir), died within 6 days of infection. In contrast, all 5 rabbits inoculated with 458[DELTA]vir gained weight normally and none developed any clinical symptoms during the entire course monitored. These results indicate that virA plays an important role in the pathogenicity of S. suis serotype 2. To investigate the virA gene further, we compared and analyzed the sequences of virA from wild-type virulent and avirulent strains. Results showed that functional virA appears to exist only in virulent strains and has been structurally mutated in avirulent strains. In conclusion, virA gene is a novel identified virulence-relate gene. It may play a key role in the evolution and pathogenicity of S. suis serotype 2. Author Affiliation: (a) College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, PR China (b) Institute of Veterinary Science, The Academy of Military Medical Sciences, Changchun 130062, PR China (c) Key laboratory of Jilin province for zoonosis prevention and control, Changchun 130062, PR China (d) State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, PR China (e) Harbin Veterinary Institute, Chinese Academy of Agriculture Sciences, Harbin 150001, PR China Article History: Received 7 December 2009; Revised 30 June 2010; Accepted 3 July 2010
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abstractTo link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.micpath.2010.07.001 Byline: Peng Li (a)(b)(c), Jun Liu (b)(c), Lingwei Zhu (b)(c), Chong Qi (b)(c), Weicheng Bei (d), Xuehui Cai (e), Yang Sun (b)(c), Shuzhang Feng (b)(c) Abstract: Streptococcus suis serotype 2 is an important porcine and human zoonotic pathogen. Few virulence factors have been identified and the pathogenesis of infection is not fully understood. We have identified a novel virulence-related gene, virA, of S. suis 2. To investigate its role in pathogenesis, a virA deletion mutant of S. suis 2 wild-type strain ZY458, 458[DELTA]vir, was constructed using suicide vector pSET4s, and a functionally complemented strain 458[DELTA]vir(pvir) was constructed using shuttle plasmid pAT18 containing the virA gene. All rabbits infected with ZY458 and 458[DELTA]vir(pvir) exhibited body weight loss and developed severe clinical symptoms. All 5 rabbits infected with ZY458, and 4 of 5 infected with 458[DELTA]vir(pvir), died within 6 days of infection. In contrast, all 5 rabbits inoculated with 458[DELTA]vir gained weight normally and none developed any clinical symptoms during the entire course monitored. These results indicate that virA plays an important role in the pathogenicity of S. suis serotype 2. To investigate the virA gene further, we compared and analyzed the sequences of virA from wild-type virulent and avirulent strains. Results showed that functional virA appears to exist only in virulent strains and has been structurally mutated in avirulent strains. In conclusion, virA gene is a novel identified virulence-relate gene. It may play a key role in the evolution and pathogenicity of S. suis serotype 2. Author Affiliation: (a) College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, PR China (b) Institute of Veterinary Science, The Academy of Military Medical Sciences, Changchun 130062, PR China (c) Key laboratory of Jilin province for zoonosis prevention and control, Changchun 130062, PR China (d) State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, PR China (e) Harbin Veterinary Institute, Chinese Academy of Agriculture Sciences, Harbin 150001, PR China Article History: Received 7 December 2009; Revised 30 June 2010; Accepted 3 July 2010
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