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Chromosome organization by a nucleoid-associated protein in live bacteria.(REPORTS)(Author abstract)(Report)

Bacterial chromosomes are confined in submicrometer-sized nucleoids. Chromosome organization is facilitated by nucleoid-associated proteins (NAPs), but the mechanisms of action remain elusive. In this work, we used super-resolution fluorescence microscopy, in combination with a chromosome-conformati... Full description

Journal Title: Science Sept 9, 2011, Vol.333(6048), p.1445(5)
Main Author: Wang, Wenqin
Other Authors: Li, Gene - Wei , Chen, Chongyi , Xie, X. Sunney , Zhuang, Xiaowei
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 0036-8075
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recordid: gale_ofa268478455
title: Chromosome organization by a nucleoid-associated protein in live bacteria.(REPORTS)(Author abstract)(Report)
format: Article
creator:
  • Wang, Wenqin
  • Li, Gene - Wei
  • Chen, Chongyi
  • Xie, X. Sunney
  • Zhuang, Xiaowei
subjects:
  • Chromosomes -- Properties
  • Cellular Proteins -- Properties
  • Bacterial Genetics -- Research
ispartof: Science, Sept 9, 2011, Vol.333(6048), p.1445(5)
description: Bacterial chromosomes are confined in submicrometer-sized nucleoids. Chromosome organization is facilitated by nucleoid-associated proteins (NAPs), but the mechanisms of action remain elusive. In this work, we used super-resolution fluorescence microscopy, in combination with a chromosome-conformation capture assay, to study the distributions of major NAPs in Live Escherichia coli cells. Four NAPs--HU, Fis, IHF, and StpA--were largely scattered throughout the nucleoid. In contrast, H-NS, a global transcriptional silencer, formed two compact clusters per chromosome, driven by oligomerization of DNA-bound H-NS through interactions mediated by the amino-terminal domain of the protein. H-NS sequestered the regulated operons into these dusters and juxtaposed numerous DNA segments broadly distributed throughout the chromosome. Deleting H-NS led to substantial chromosome reorganization. These observations demonstrate that H-NS plays a key rote in global chromosome organization in bacteria. 10.1126/science.1204697
language: English
source:
identifier: ISSN: 0036-8075
fulltext: fulltext
issn:
  • 0036-8075
  • 00368075
url: Link


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titleChromosome organization by a nucleoid-associated protein in live bacteria.(REPORTS)(Author abstract)(Report)
creatorWang, Wenqin ; Li, Gene - Wei ; Chen, Chongyi ; Xie, X. Sunney ; Zhuang, Xiaowei
ispartofScience, Sept 9, 2011, Vol.333(6048), p.1445(5)
identifierISSN: 0036-8075
subjectChromosomes -- Properties ; Cellular Proteins -- Properties ; Bacterial Genetics -- Research
descriptionBacterial chromosomes are confined in submicrometer-sized nucleoids. Chromosome organization is facilitated by nucleoid-associated proteins (NAPs), but the mechanisms of action remain elusive. In this work, we used super-resolution fluorescence microscopy, in combination with a chromosome-conformation capture assay, to study the distributions of major NAPs in Live Escherichia coli cells. Four NAPs--HU, Fis, IHF, and StpA--were largely scattered throughout the nucleoid. In contrast, H-NS, a global transcriptional silencer, formed two compact clusters per chromosome, driven by oligomerization of DNA-bound H-NS through interactions mediated by the amino-terminal domain of the protein. H-NS sequestered the regulated operons into these dusters and juxtaposed numerous DNA segments broadly distributed throughout the chromosome. Deleting H-NS led to substantial chromosome reorganization. These observations demonstrate that H-NS plays a key rote in global chromosome organization in bacteria. 10.1126/science.1204697
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titleChromosome organization by a nucleoid-associated protein in live bacteria.(REPORTS)(Author abstract)(Report)
descriptionBacterial chromosomes are confined in submicrometer-sized nucleoids. Chromosome organization is facilitated by nucleoid-associated proteins (NAPs), but the mechanisms of action remain elusive. In this work, we used super-resolution fluorescence microscopy, in combination with a chromosome-conformation capture assay, to study the distributions of major NAPs in Live Escherichia coli cells. Four NAPs--HU, Fis, IHF, and StpA--were largely scattered throughout the nucleoid. In contrast, H-NS, a global transcriptional silencer, formed two compact clusters per chromosome, driven by oligomerization of DNA-bound H-NS through interactions mediated by the amino-terminal domain of the protein. H-NS sequestered the regulated operons into these dusters and juxtaposed numerous DNA segments broadly distributed throughout the chromosome. Deleting H-NS led to substantial chromosome reorganization. These observations demonstrate that H-NS plays a key rote in global chromosome organization in bacteria. 10.1126/science.1204697
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titleChromosome organization by a nucleoid-associated protein in live bacteria.(REPORTS)(Author abstract)(Report)
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abstractBacterial chromosomes are confined in submicrometer-sized nucleoids. Chromosome organization is facilitated by nucleoid-associated proteins (NAPs), but the mechanisms of action remain elusive. In this work, we used super-resolution fluorescence microscopy, in combination with a chromosome-conformation capture assay, to study the distributions of major NAPs in Live Escherichia coli cells. Four NAPs--HU, Fis, IHF, and StpA--were largely scattered throughout the nucleoid. In contrast, H-NS, a global transcriptional silencer, formed two compact clusters per chromosome, driven by oligomerization of DNA-bound H-NS through interactions mediated by the amino-terminal domain of the protein. H-NS sequestered the regulated operons into these dusters and juxtaposed numerous DNA segments broadly distributed throughout the chromosome. Deleting H-NS led to substantial chromosome reorganization. These observations demonstrate that H-NS plays a key rote in global chromosome organization in bacteria. 10.1126/science.1204697
pubAmerican Association for the Advancement of Science
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doi10.1126/science.1204697
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