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Surface modification of three-dimensional Ca-P/PHBV nanocomposite scaffolds by physical entrapment of gelatin and its in vitro biological evaluation.(Report)

Byline: Bin Duan (1), Min Wang (1), Zhao Yang Li (2), Wai Chun Chan (1), William W. Lu (2) Keywords: nanocomposite scaffold; selective laser sintering; surface modification; physical entrapment; cell behaviour Abstract: The properties of bone tissue engineering scaffolds such as architecture, porosi... Full description

Journal Title: Frontiers of Materials Science March, 2011, Vol.5(1), p.57(12)
Main Author: Duan, Bin
Other Authors: Wang, Min , Li, Zhao Yang , Chan, Wai Chun , Lu, William W.
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 2095-025X
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recordid: gale_ofa280335949
title: Surface modification of three-dimensional Ca-P/PHBV nanocomposite scaffolds by physical entrapment of gelatin and its in vitro biological evaluation.(Report)
format: Article
creator:
  • Duan, Bin
  • Wang, Min
  • Li, Zhao Yang
  • Chan, Wai Chun
  • Lu, William W.
subjects:
  • Universities And Colleges -- Mechanical Properties
  • Phosphates -- Mechanical Properties
  • Copolymers -- Mechanical Properties
  • Calcium Phosphate -- Mechanical Properties
  • Tissue Engineering -- Mechanical Properties
  • Porosity -- Mechanical Properties
  • Rapid Prototyping -- Mechanical Properties
  • Sintering -- Mechanical Properties
ispartof: Frontiers of Materials Science, March, 2011, Vol.5(1), p.57(12)
description: Byline: Bin Duan (1), Min Wang (1), Zhao Yang Li (2), Wai Chun Chan (1), William W. Lu (2) Keywords: nanocomposite scaffold; selective laser sintering; surface modification; physical entrapment; cell behaviour Abstract: The properties of bone tissue engineering scaffolds such as architecture, porosity, mechanical properties and surface properties have significant effects on cellular response and play an important role in bone regeneration. In this study, threedimensional nanocomposite scaffolds consisting of calcium phosphate (Ca-P) nanoparticles and poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) copolymer with controlled external and internal architectures were successfully produced via selective laser sintering (SLS), one of the versatile rapid prototyping techniques. The Ca-P/PHBV nanocomposite scaffolds had a porosity of (61.75+-1.24)%, compressive strength of (2.16+-0.21) MPa and Young's modulus of (26.98+-2.29) MPa. The surface modification of scaffolds by gelatin was achieved through physical entrapment. The amount of entrapped gelatin could be controlled by varying the solvent composition and reaction time. The surface modification improved the hydrophilicity of scaffolds but did not significantly affect the surface morphology and mechanical properties. Osteoblast-like cells (SaOS-2) were cultured on scaffolds with and without gelatin surface modification. The majority of SaOS-2 cells were viable and proliferated in both types of scaffolds for up to 14 d in culture, as indicated by MTT assay and live and dead assay. Surface modification significantly increased cell proliferation for surface modified scaffolds, which could be due to the improvement in hydrophilicity of the scaffolds. Author Affiliation: (1) Department of Mechanical Engineering, Faculty of Engineering, The University of Hong Kong, Pokfulam Road, Hong Kong, China (2) Department of Orthopaedics and Traumatology, Faculty of Medicine, The University of Hong Kong, Sassoon Road, Hong Kong, China Article History: Registration Date: 28/09/2011 Received Date: 10/08/2010 Accepted Date: 25/08/2010 Online Date: 28/09/2010
language: English
source:
identifier: ISSN: 2095-025X
fulltext: fulltext
issn:
  • 2095-025X
  • 2095025X
url: Link


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titleSurface modification of three-dimensional Ca-P/PHBV nanocomposite scaffolds by physical entrapment of gelatin and its in vitro biological evaluation.(Report)
creatorDuan, Bin ; Wang, Min ; Li, Zhao Yang ; Chan, Wai Chun ; Lu, William W.
ispartofFrontiers of Materials Science, March, 2011, Vol.5(1), p.57(12)
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subjectUniversities And Colleges -- Mechanical Properties ; Phosphates -- Mechanical Properties ; Copolymers -- Mechanical Properties ; Calcium Phosphate -- Mechanical Properties ; Tissue Engineering -- Mechanical Properties ; Porosity -- Mechanical Properties ; Rapid Prototyping -- Mechanical Properties ; Sintering -- Mechanical Properties
descriptionByline: Bin Duan (1), Min Wang (1), Zhao Yang Li (2), Wai Chun Chan (1), William W. Lu (2) Keywords: nanocomposite scaffold; selective laser sintering; surface modification; physical entrapment; cell behaviour Abstract: The properties of bone tissue engineering scaffolds such as architecture, porosity, mechanical properties and surface properties have significant effects on cellular response and play an important role in bone regeneration. In this study, threedimensional nanocomposite scaffolds consisting of calcium phosphate (Ca-P) nanoparticles and poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) copolymer with controlled external and internal architectures were successfully produced via selective laser sintering (SLS), one of the versatile rapid prototyping techniques. The Ca-P/PHBV nanocomposite scaffolds had a porosity of (61.75+-1.24)%, compressive strength of (2.16+-0.21) MPa and Young's modulus of (26.98+-2.29) MPa. The surface modification of scaffolds by gelatin was achieved through physical entrapment. The amount of entrapped gelatin could be controlled by varying the solvent composition and reaction time. The surface modification improved the hydrophilicity of scaffolds but did not significantly affect the surface morphology and mechanical properties. Osteoblast-like cells (SaOS-2) were cultured on scaffolds with and without gelatin surface modification. The majority of SaOS-2 cells were viable and proliferated in both types of scaffolds for up to 14 d in culture, as indicated by MTT assay and live and dead assay. Surface modification significantly increased cell proliferation for surface modified scaffolds, which could be due to the improvement in hydrophilicity of the scaffolds. Author Affiliation: (1) Department of Mechanical Engineering, Faculty of Engineering, The University of Hong Kong, Pokfulam Road, Hong Kong, China (2) Department of Orthopaedics and Traumatology, Faculty of Medicine, The University of Hong Kong, Sassoon Road, Hong Kong, China Article History: Registration Date: 28/09/2011 Received Date: 10/08/2010 Accepted Date: 25/08/2010 Online Date: 28/09/2010
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titleSurface modification of three-dimensional Ca-P/PHBV nanocomposite scaffolds by physical entrapment of gelatin and its in vitro biological evaluation.(Report)
descriptionByline: Bin Duan (1), Min Wang (1), Zhao Yang Li (2), Wai Chun Chan (1), William W. Lu (2) Keywords: nanocomposite scaffold; selective laser sintering; surface modification; physical entrapment; cell behaviour Abstract: The properties of bone tissue engineering scaffolds such as architecture, porosity, mechanical properties and surface properties have significant effects on cellular response and play an important role in bone regeneration. In this study, threedimensional nanocomposite scaffolds consisting of calcium phosphate (Ca-P) nanoparticles and poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) copolymer with controlled external and internal architectures were successfully produced via selective laser sintering (SLS), one of the versatile rapid prototyping techniques. The Ca-P/PHBV nanocomposite scaffolds had a porosity of (61.75+-1.24)%, compressive strength of (2.16+-0.21) MPa and Young's modulus of (26.98+-2.29) MPa. The surface modification of scaffolds by gelatin was achieved through physical entrapment. The amount of entrapped gelatin could be controlled by varying the solvent composition and reaction time. The surface modification improved the hydrophilicity of scaffolds but did not significantly affect the surface morphology and mechanical properties. Osteoblast-like cells (SaOS-2) were cultured on scaffolds with and without gelatin surface modification. The majority of SaOS-2 cells were viable and proliferated in both types of scaffolds for up to 14 d in culture, as indicated by MTT assay and live and dead assay. Surface modification significantly increased cell proliferation for surface modified scaffolds, which could be due to the improvement in hydrophilicity of the scaffolds. Author Affiliation: (1) Department of Mechanical Engineering, Faculty of Engineering, The University of Hong Kong, Pokfulam Road, Hong Kong, China (2) Department of Orthopaedics and Traumatology, Faculty of Medicine, The University of Hong Kong, Sassoon Road, Hong Kong, China Article History: Registration Date: 28/09/2011 Received Date: 10/08/2010 Accepted Date: 25/08/2010 Online Date: 28/09/2010
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abstractByline: Bin Duan (1), Min Wang (1), Zhao Yang Li (2), Wai Chun Chan (1), William W. Lu (2) Keywords: nanocomposite scaffold; selective laser sintering; surface modification; physical entrapment; cell behaviour Abstract: The properties of bone tissue engineering scaffolds such as architecture, porosity, mechanical properties and surface properties have significant effects on cellular response and play an important role in bone regeneration. In this study, threedimensional nanocomposite scaffolds consisting of calcium phosphate (Ca-P) nanoparticles and poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) copolymer with controlled external and internal architectures were successfully produced via selective laser sintering (SLS), one of the versatile rapid prototyping techniques. The Ca-P/PHBV nanocomposite scaffolds had a porosity of (61.75+-1.24)%, compressive strength of (2.16+-0.21) MPa and Young's modulus of (26.98+-2.29) MPa. The surface modification of scaffolds by gelatin was achieved through physical entrapment. The amount of entrapped gelatin could be controlled by varying the solvent composition and reaction time. The surface modification improved the hydrophilicity of scaffolds but did not significantly affect the surface morphology and mechanical properties. Osteoblast-like cells (SaOS-2) were cultured on scaffolds with and without gelatin surface modification. The majority of SaOS-2 cells were viable and proliferated in both types of scaffolds for up to 14 d in culture, as indicated by MTT assay and live and dead assay. Surface modification significantly increased cell proliferation for surface modified scaffolds, which could be due to the improvement in hydrophilicity of the scaffolds. Author Affiliation: (1) Department of Mechanical Engineering, Faculty of Engineering, The University of Hong Kong, Pokfulam Road, Hong Kong, China (2) Department of Orthopaedics and Traumatology, Faculty of Medicine, The University of Hong Kong, Sassoon Road, Hong Kong, China Article History: Registration Date: 28/09/2011 Received Date: 10/08/2010 Accepted Date: 25/08/2010 Online Date: 28/09/2010
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