schliessen

Filtern

 

Bibliotheken

A modified LC-MS/MS method for determination of tetramethylpyrazine in microdialysis samples and calibration of home-made linear probes

Byline: Jin-Zhao Han, Jun Sun, Quan-Gang Zhu, Ji-Yong Liu, Jin-Hong Hu, Fang Chen ABSTRACT Tetramethylpyrazine (TMP) is one of the most important active ingredients of a Chinese herb Ligusticum wallichii Franchat, which is widely used for the treatment of cardiovascular diseases. Several factors may... Full description

Journal Title: Biomedical Chromatography Oct, 2012, Vol.26(10), p.1276(6)
Main Author: Han, Jin - Zhao
Other Authors: Sun, Jun , Zhu, Quan - Gang , Liu, Ji - Yong , Hu, Jin - Hong , Chen, Fang
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 0269-3879
Zum Text:
SendSend as email Add to Book BagAdd to Book Bag
Staff View
recordid: gale_ofa318045954
title: A modified LC-MS/MS method for determination of tetramethylpyrazine in microdialysis samples and calibration of home-made linear probes
format: Article
creator:
  • Han, Jin - Zhao
  • Sun, Jun
  • Zhu, Quan - Gang
  • Liu, Ji - Yong
  • Hu, Jin - Hong
  • Chen, Fang
subjects:
  • Herbal Medicine -- Methods
  • Herbal Medicine -- Analysis
  • Herbal Medicine -- Investigations
ispartof: Biomedical Chromatography, Oct, 2012, Vol.26(10), p.1276(6)
description: Byline: Jin-Zhao Han, Jun Sun, Quan-Gang Zhu, Ji-Yong Liu, Jin-Hong Hu, Fang Chen ABSTRACT Tetramethylpyrazine (TMP) is one of the most important active ingredients of a Chinese herb Ligusticum wallichii Franchat, which is widely used for the treatment of cardiovascular diseases. Several factors may affect TMP exposure after topical administration, resulting in large variability and demanding further elucidation of drug distribution. This paper describes a new efficient reliable LC-MS/MS assay for the determination of TMP in dermal microdialysate, where TMP was separated on an Agilent C.sub.18 column (3.5A[micro]m, 100mmx2.1mm i.d.) using a mixture of methanol, water and acetic acid (50:50:0.6, v/v/v) at a flow-rate of 0.3mL/min. The retention time was 1.89min for TMP and 1.17min for the internal standard (caffeine). Histological analysis confirmed an inflammatory response to the microdialysis probes and the presence of a collagen capsule. The membrane extraction efficiency (percentage delivered to the tissue space) for TMP was not altered through the implant lifetime. The validation and sample analysis results showed that the method is precise, accurate and well suited to support dermal microdialysis experiments. Copyright A[c] 2012 John Wiley & Sons, Ltd. Correspondence: Jin-Hong Hu, Department of Pharmacy, Changhai Hospital, Second Military Medical University, Chang Hai Road 168, Shanghai 200433, People's Republic of China. E-mail: hjhong0066@gmail.com
language: English
source:
identifier: ISSN: 0269-3879
fulltext: fulltext
issn:
  • 0269-3879
  • 02693879
url: Link


@attributes
ID603246534
RANK0.07
NO1
SEARCH_ENGINEprimo_central_multiple_fe
SEARCH_ENGINE_TYPEPrimo Central Search Engine
LOCALfalse
PrimoNMBib
record
control
sourcerecordid318045954
sourceidgale_ofa
recordidTN_gale_ofa318045954
sourceformatXML
sourcesystemPC
pqid1038070342
galeid318045954
display
typearticle
titleA modified LC-MS/MS method for determination of tetramethylpyrazine in microdialysis samples and calibration of home-made linear probes
creatorHan, Jin - Zhao ; Sun, Jun ; Zhu, Quan - Gang ; Liu, Ji - Yong ; Hu, Jin - Hong ; Chen, Fang
ispartofBiomedical Chromatography, Oct, 2012, Vol.26(10), p.1276(6)
identifierISSN: 0269-3879
subjectHerbal Medicine -- Methods ; Herbal Medicine -- Analysis ; Herbal Medicine -- Investigations
descriptionByline: Jin-Zhao Han, Jun Sun, Quan-Gang Zhu, Ji-Yong Liu, Jin-Hong Hu, Fang Chen ABSTRACT Tetramethylpyrazine (TMP) is one of the most important active ingredients of a Chinese herb Ligusticum wallichii Franchat, which is widely used for the treatment of cardiovascular diseases. Several factors may affect TMP exposure after topical administration, resulting in large variability and demanding further elucidation of drug distribution. This paper describes a new efficient reliable LC-MS/MS assay for the determination of TMP in dermal microdialysate, where TMP was separated on an Agilent C.sub.18 column (3.5A[micro]m, 100mmx2.1mm i.d.) using a mixture of methanol, water and acetic acid (50:50:0.6, v/v/v) at a flow-rate of 0.3mL/min. The retention time was 1.89min for TMP and 1.17min for the internal standard (caffeine). Histological analysis confirmed an inflammatory response to the microdialysis probes and the presence of a collagen capsule. The membrane extraction efficiency (percentage delivered to the tissue space) for TMP was not altered through the implant lifetime. The validation and sample analysis results showed that the method is precise, accurate and well suited to support dermal microdialysis experiments. Copyright A[c] 2012 John Wiley & Sons, Ltd. Correspondence: Jin-Hong Hu, Department of Pharmacy, Changhai Hospital, Second Military Medical University, Chang Hai Road 168, Shanghai 200433, People's Republic of China. E-mail: hjhong0066@gmail.com
languageEnglish
source
version6
lds50peer_reviewed
links
openurl$$Topenurl_article
openurlfulltext$$Topenurlfull_article
search
scope
0gale_onefilea
1OneFile
creatorcontrib
0Han, Jin-Zhao
1Jin-Zhao Han
2Sun, Jun
3Zhu, Quan-Gang
4Liu, Ji-Yong
5Hu, Jin-Hong
6Chen, Fang
titleA modified LC-MS/MS method for determination of tetramethylpyrazine in microdialysis samples and calibration of home-made linear probes.
descriptionByline: Jin-Zhao Han, Jun Sun, Quan-Gang Zhu, Ji-Yong Liu, Jin-Hong Hu, Fang Chen ABSTRACT Tetramethylpyrazine (TMP) is one of the most important active ingredients of a Chinese herb Ligusticum wallichii Franchat, which is widely used for the treatment of cardiovascular diseases. Several factors may affect TMP exposure after topical administration, resulting in large variability and demanding further elucidation of drug distribution. This paper describes a new efficient reliable LC-MS/MS assay for the determination of TMP in dermal microdialysate, where TMP was separated on an Agilent C.sub.18 column (3.5A[micro]m, 100mmx2.1mm i.d.) using a mixture of methanol, water and acetic acid (50:50:0.6, v/v/v) at a flow-rate of 0.3mL/min. The retention time was 1.89min for TMP and 1.17min for the internal standard (caffeine). Histological analysis confirmed an inflammatory response to the microdialysis probes and the presence of a collagen capsule. The membrane extraction efficiency (percentage delivered to the tissue space) for TMP was not altered through the implant lifetime. The validation and sample analysis results showed that the method is precise, accurate and well suited to support dermal microdialysis experiments. Copyright A[c] 2012 John Wiley & Sons, Ltd. Correspondence: Jin-Hong Hu, Department of Pharmacy, Changhai Hospital, Second Military Medical University, Chang Hai Road 168, Shanghai 200433, People's Republic of China. E-mail: hjhong0066@gmail.com
subject
0Herbal medicine--Methods
1Herbal medicine--Analysis
2Herbal medicine--Investigations
3Company legal issue
general
0English
1Wiley Subscription Services, Inc.
2Cengage Learning, Inc.
sourceidgale_ofa
recordidgale_ofa318045954
issn
00269-3879
102693879
rsrctypearticle
creationdate2012
recordtypearticle
addtitleBiomedical Chromatography
searchscopeOneFile
lsr30VSR-Enriched:[galeid, pqid, eissn, doi, pages, date]
sort
titleA modified LC-MS/MS method for determination of tetramethylpyrazine in microdialysis samples and calibration of home-made linear probes.
authorHan, Jin - Zhao ; Sun, Jun ; Zhu, Quan - Gang ; Liu, Ji - Yong ; Hu, Jin - Hong ; Chen, Fang
creationdate20121001
facets
frbrgroupid7362582064434793020
frbrtype5
languageeng
creationdate2012
topic
0Herbal Medicine–Methods
1Herbal Medicine–Analysis
2Herbal Medicine–Investigations
collectionOneFile (GALE)
prefilterarticles
rsrctypearticles
creatorcontrib
0Han, Jin - Zhao
1Sun, Jun
2Zhu, Quan - Gang
3Liu, Ji - Yong
4Hu, Jin - Hong
5Chen, Fang
jtitleBiomedical Chromatography
toplevelpeer_reviewed
delivery
delcategoryRemote Search Resource
fulltextfulltext
addata
au
0Han, Jin-Zhao
1Sun, Jun
2Zhu, Quan-Gang
3Liu, Ji-Yong
4Hu, Jin-Hong
5Chen, Fang
atitleA modified LC-MS/MS method for determination of tetramethylpyrazine in microdialysis samples and calibration of home-made linear probes.
jtitleBiomedical Chromatography
risdate20121001
volume26
issue10
spage1276
issn0269-3879
genrearticle
ristypeJOUR
abstractByline: Jin-Zhao Han, Jun Sun, Quan-Gang Zhu, Ji-Yong Liu, Jin-Hong Hu, Fang Chen ABSTRACT Tetramethylpyrazine (TMP) is one of the most important active ingredients of a Chinese herb Ligusticum wallichii Franchat, which is widely used for the treatment of cardiovascular diseases. Several factors may affect TMP exposure after topical administration, resulting in large variability and demanding further elucidation of drug distribution. This paper describes a new efficient reliable LC-MS/MS assay for the determination of TMP in dermal microdialysate, where TMP was separated on an Agilent C.sub.18 column (3.5A[micro]m, 100mmx2.1mm i.d.) using a mixture of methanol, water and acetic acid (50:50:0.6, v/v/v) at a flow-rate of 0.3mL/min. The retention time was 1.89min for TMP and 1.17min for the internal standard (caffeine). Histological analysis confirmed an inflammatory response to the microdialysis probes and the presence of a collagen capsule. The membrane extraction efficiency (percentage delivered to the tissue space) for TMP was not altered through the implant lifetime. The validation and sample analysis results showed that the method is precise, accurate and well suited to support dermal microdialysis experiments. Copyright A[c] 2012 John Wiley & Sons, Ltd. Correspondence: Jin-Hong Hu, Department of Pharmacy, Changhai Hospital, Second Military Medical University, Chang Hai Road 168, Shanghai 200433, People's Republic of China. E-mail: hjhong0066@gmail.com
pubWiley Subscription Services, Inc.
lad01gale_ofa
doi10.1002/bmc.2689
pages1276-1281
eissn10990801
date2012-10