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Amniotic membrane extract ameliorates benzalkonium chloride-induced dry eye in a murine model

To link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.exer.2013.06.005 Byline: Xinye Xiao, Pingping Luo, Hui Zhao, Jingyao Chen, Hui He, Yuxue Xu, Zhirong Lin, Yueping Zhou, Jianjiang Xu, Zuguo Liu Abstract: Human amniotic membrane (AM) is avascular but contains... Full description

Journal Title: Experimental Eye Research Oct, 2013, Vol.115, p.31(10)
Main Author: Xiao, Xinye
Other Authors: Luo, Pingping , Zhao, Hui , Chen, Jingyao , He, Hui , Xu, Yuxue , Lin, Zhirong , Zhou, Yueping , Xu, Jianjiang , Liu, Zuguo
Format: Electronic Article Electronic Article
Language: English
Subjects:
Quelle: Cengage Learning, Inc.
ID: ISSN: 0014-4835
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recordid: gale_ofa345795789
title: Amniotic membrane extract ameliorates benzalkonium chloride-induced dry eye in a murine model
format: Article
creator:
  • Xiao, Xinye
  • Luo, Pingping
  • Zhao, Hui
  • Chen, Jingyao
  • He, Hui
  • Xu, Yuxue
  • Lin, Zhirong
  • Zhou, Yueping
  • Xu, Jianjiang
  • Liu, Zuguo
subjects:
  • Surface Active Agents -- Analysis
  • Fluorescein -- Analysis
  • Ophthalmology -- Analysis
ispartof: Experimental Eye Research, Oct, 2013, Vol.115, p.31(10)
description: To link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.exer.2013.06.005 Byline: Xinye Xiao, Pingping Luo, Hui Zhao, Jingyao Chen, Hui He, Yuxue Xu, Zhirong Lin, Yueping Zhou, Jianjiang Xu, Zuguo Liu Abstract: Human amniotic membrane (AM) is avascular but contains various beneficial bioactive factors, its extract (AE) is also effective in treating many ocular surface disorders. In this study, we for the first time evaluated the therapeutic effects of AE on dry eye induced by benzalkonium chloride in a BALB/c mouse model. Topical application of AE (1.5 and 3 [mu]g/eye/day) resulted in significantly longer tear break-up time on Day 3 and 6, lower fluorescein staining scores on Day 3, and lower inflammatory index on Day 6. AE reduced corneal epithelial K10 expression, inflammatory infiltration, and levels of TNF-[alpha], IL-1[beta] and IL-6 in BAC treated mice than that in the control mice. Moreover, decreased TUNEL positive cells in cornea and increased goblet cells in conjunctiva were also observed in AE treated corneas. Finally, AE induced more Ki-67 positive cells in corneal epithelium of dry eye mouse. Taken together, our data provide further support for BAC induced dry eye model as a valuable for dry eye study and suggest a great potential for AE as a therapeutic agent in the clinical treatment of dry eye. Author Affiliation: (a) Eye Institute of Xiamen University, Xiamen, PR China (b) Department of Ophthalmology, The First Affiliated Hospital of Xiamen University, Xiamen, PR China (c) The Affiliated Xiamen Eye Center of Xiamen University, Xiamen, PR China (d) Department of Ophthalmology, Eye & ENT Hospital of Fudan University, Shanghai, PR China Article History: Received 21 March 2013; Accepted 6 June 2013
language: English
source: Cengage Learning, Inc.
identifier: ISSN: 0014-4835
fulltext: fulltext
issn:
  • 0014-4835
  • 00144835
url: Link


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titleAmniotic membrane extract ameliorates benzalkonium chloride-induced dry eye in a murine model
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descriptionTo link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.exer.2013.06.005 Byline: Xinye Xiao, Pingping Luo, Hui Zhao, Jingyao Chen, Hui He, Yuxue Xu, Zhirong Lin, Yueping Zhou, Jianjiang Xu, Zuguo Liu Abstract: Human amniotic membrane (AM) is avascular but contains various beneficial bioactive factors, its extract (AE) is also effective in treating many ocular surface disorders. In this study, we for the first time evaluated the therapeutic effects of AE on dry eye induced by benzalkonium chloride in a BALB/c mouse model. Topical application of AE (1.5 and 3 [mu]g/eye/day) resulted in significantly longer tear break-up time on Day 3 and 6, lower fluorescein staining scores on Day 3, and lower inflammatory index on Day 6. AE reduced corneal epithelial K10 expression, inflammatory infiltration, and levels of TNF-[alpha], IL-1[beta] and IL-6 in BAC treated mice than that in the control mice. Moreover, decreased TUNEL positive cells in cornea and increased goblet cells in conjunctiva were also observed in AE treated corneas. Finally, AE induced more Ki-67 positive cells in corneal epithelium of dry eye mouse. Taken together, our data provide further support for BAC induced dry eye model as a valuable for dry eye study and suggest a great potential for AE as a therapeutic agent in the clinical treatment of dry eye. Author Affiliation: (a) Eye Institute of Xiamen University, Xiamen, PR China (b) Department of Ophthalmology, The First Affiliated Hospital of Xiamen University, Xiamen, PR China (c) The Affiliated Xiamen Eye Center of Xiamen University, Xiamen, PR China (d) Department of Ophthalmology, Eye & ENT Hospital of Fudan University, Shanghai, PR China Article History: Received 21 March 2013; Accepted 6 June 2013
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abstractTo link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.exer.2013.06.005 Byline: Xinye Xiao, Pingping Luo, Hui Zhao, Jingyao Chen, Hui He, Yuxue Xu, Zhirong Lin, Yueping Zhou, Jianjiang Xu, Zuguo Liu Abstract: Human amniotic membrane (AM) is avascular but contains various beneficial bioactive factors, its extract (AE) is also effective in treating many ocular surface disorders. In this study, we for the first time evaluated the therapeutic effects of AE on dry eye induced by benzalkonium chloride in a BALB/c mouse model. Topical application of AE (1.5 and 3 [mu]g/eye/day) resulted in significantly longer tear break-up time on Day 3 and 6, lower fluorescein staining scores on Day 3, and lower inflammatory index on Day 6. AE reduced corneal epithelial K10 expression, inflammatory infiltration, and levels of TNF-[alpha], IL-1[beta] and IL-6 in BAC treated mice than that in the control mice. Moreover, decreased TUNEL positive cells in cornea and increased goblet cells in conjunctiva were also observed in AE treated corneas. Finally, AE induced more Ki-67 positive cells in corneal epithelium of dry eye mouse. Taken together, our data provide further support for BAC induced dry eye model as a valuable for dry eye study and suggest a great potential for AE as a therapeutic agent in the clinical treatment of dry eye. Author Affiliation: (a) Eye Institute of Xiamen University, Xiamen, PR China (b) Department of Ophthalmology, The First Affiliated Hospital of Xiamen University, Xiamen, PR China (c) The Affiliated Xiamen Eye Center of Xiamen University, Xiamen, PR China (d) Department of Ophthalmology, Eye & ENT Hospital of Fudan University, Shanghai, PR China Article History: Received 21 March 2013; Accepted 6 June 2013
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