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The complete replacement of antibodies by protein-imprinted poly(ethylene-co-vinyl alcohol) in sandwich fluoroimmunoassays.(Report)

Byline: Mei-Hwa Lee (1), James L. Thomas (2), Yun-Chao Chen (3), Wei-Ti Chin (1), Hung-Yin Lin (3) Keywords: Molecular imprinting; Sandwich fluoroimmunoassays; Salivary proteins; Poly(ethylene-co-vinyl alcohol) Abstract: The replacement of antibodies by molecularly imprinted polymers (MIPs) has been... Full description

Journal Title: Microchimica Acta Nov, 2013, Vol.180(15-16), p.1393(7)
Main Author: Lee, Mei - Hwa
Other Authors: Thomas, James L. , Chen, Yun - Chao , Chin, Wei - Ti , Lin, Hung - Yin
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 0026-3672
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recordid: gale_ofa348523867
title: The complete replacement of antibodies by protein-imprinted poly(ethylene-co-vinyl alcohol) in sandwich fluoroimmunoassays.(Report)
format: Article
creator:
  • Lee, Mei - Hwa
  • Thomas, James L.
  • Chen, Yun - Chao
  • Chin, Wei - Ti
  • Lin, Hung - Yin
subjects:
  • Thin Films
  • Lysozyme
  • Lipase
  • Ethylene
  • Proteins
  • Antibodies
ispartof: Microchimica Acta, Nov, 2013, Vol.180(15-16), p.1393(7)
description: Byline: Mei-Hwa Lee (1), James L. Thomas (2), Yun-Chao Chen (3), Wei-Ti Chin (1), Hung-Yin Lin (3) Keywords: Molecular imprinting; Sandwich fluoroimmunoassays; Salivary proteins; Poly(ethylene-co-vinyl alcohol) Abstract: The replacement of antibodies by molecularly imprinted polymers (MIPs) has been investigated for many decades. However, indirect protocols (including natural primary and secondary antibodies) are still utilized to evaluate the ability of MIP thin films to recognize target molecules. MIPs can be prepared as either a thin film or as particles, and cavities that are complementary to the template can be generated on their surfaces. We have prepared thin film MIPs and particle MIPs prepared by solvent evaporation and phase inversion, respectively, from solutions of poly(ethylene-co-vinyl alcohol) (pEVAL) in the presence of the target analytes amylase, lysozyme, and lipase. These were first adsorbed on MIP thin films and by MIP particles that contain fluorescent quantum dots. Sandwich fluoroimmunoassays were then conducted to quantify them in MIP-coated 96-well microplates. The method was applied to determine amylase in saliva, and results were compared with a commercial analytical system. Figure The recognition of amylase-imprinted poly(ethylene-co-vinyl alcohol)/quantum dots composite nanoparticles to amylase on the amylase-imprinted poly(ethylene-co-vinyl alcohol) coated 96-well microplates. Author Affiliation: (1) Department of Materials Science and Engineering, I-Shou University, Kaohsiung, 84001, Taiwan (2) Department of Physics and Astronomy, University of New Mexico, Albuquerque, NM, 87131, USA (3) Department of Chemical and Materials Engineering, National University of Kaohsiung (NUK), 700, Kaohsiung University Rd., Nan-Tzu District, Kaohsiung, 81148, Taiwan Article History: Registration Date: 09/04/2013 Received Date: 21/10/2012 Accepted Date: 09/04/2013 Online Date: 30/04/2013
language: English
source:
identifier: ISSN: 0026-3672
fulltext: fulltext
issn:
  • 0026-3672
  • 00263672
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titleThe complete replacement of antibodies by protein-imprinted poly(ethylene-co-vinyl alcohol) in sandwich fluoroimmunoassays.(Report)
creatorLee, Mei - Hwa ; Thomas, James L. ; Chen, Yun - Chao ; Chin, Wei - Ti ; Lin, Hung - Yin
ispartofMicrochimica Acta, Nov, 2013, Vol.180(15-16), p.1393(7)
identifierISSN: 0026-3672
subjectThin Films ; Lysozyme ; Lipase ; Ethylene ; Proteins ; Antibodies
descriptionByline: Mei-Hwa Lee (1), James L. Thomas (2), Yun-Chao Chen (3), Wei-Ti Chin (1), Hung-Yin Lin (3) Keywords: Molecular imprinting; Sandwich fluoroimmunoassays; Salivary proteins; Poly(ethylene-co-vinyl alcohol) Abstract: The replacement of antibodies by molecularly imprinted polymers (MIPs) has been investigated for many decades. However, indirect protocols (including natural primary and secondary antibodies) are still utilized to evaluate the ability of MIP thin films to recognize target molecules. MIPs can be prepared as either a thin film or as particles, and cavities that are complementary to the template can be generated on their surfaces. We have prepared thin film MIPs and particle MIPs prepared by solvent evaporation and phase inversion, respectively, from solutions of poly(ethylene-co-vinyl alcohol) (pEVAL) in the presence of the target analytes amylase, lysozyme, and lipase. These were first adsorbed on MIP thin films and by MIP particles that contain fluorescent quantum dots. Sandwich fluoroimmunoassays were then conducted to quantify them in MIP-coated 96-well microplates. The method was applied to determine amylase in saliva, and results were compared with a commercial analytical system. Figure The recognition of amylase-imprinted poly(ethylene-co-vinyl alcohol)/quantum dots composite nanoparticles to amylase on the amylase-imprinted poly(ethylene-co-vinyl alcohol) coated 96-well microplates. Author Affiliation: (1) Department of Materials Science and Engineering, I-Shou University, Kaohsiung, 84001, Taiwan (2) Department of Physics and Astronomy, University of New Mexico, Albuquerque, NM, 87131, USA (3) Department of Chemical and Materials Engineering, National University of Kaohsiung (NUK), 700, Kaohsiung University Rd., Nan-Tzu District, Kaohsiung, 81148, Taiwan Article History: Registration Date: 09/04/2013 Received Date: 21/10/2012 Accepted Date: 09/04/2013 Online Date: 30/04/2013
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titleThe complete replacement of antibodies by protein-imprinted poly(ethylene-co-vinyl alcohol) in sandwich fluoroimmunoassays.(Report)
descriptionByline: Mei-Hwa Lee (1), James L. Thomas (2), Yun-Chao Chen (3), Wei-Ti Chin (1), Hung-Yin Lin (3) Keywords: Molecular imprinting; Sandwich fluoroimmunoassays; Salivary proteins; Poly(ethylene-co-vinyl alcohol) Abstract: The replacement of antibodies by molecularly imprinted polymers (MIPs) has been investigated for many decades. However, indirect protocols (including natural primary and secondary antibodies) are still utilized to evaluate the ability of MIP thin films to recognize target molecules. MIPs can be prepared as either a thin film or as particles, and cavities that are complementary to the template can be generated on their surfaces. We have prepared thin film MIPs and particle MIPs prepared by solvent evaporation and phase inversion, respectively, from solutions of poly(ethylene-co-vinyl alcohol) (pEVAL) in the presence of the target analytes amylase, lysozyme, and lipase. These were first adsorbed on MIP thin films and by MIP particles that contain fluorescent quantum dots. Sandwich fluoroimmunoassays were then conducted to quantify them in MIP-coated 96-well microplates. The method was applied to determine amylase in saliva, and results were compared with a commercial analytical system. Figure The recognition of amylase-imprinted poly(ethylene-co-vinyl alcohol)/quantum dots composite nanoparticles to amylase on the amylase-imprinted poly(ethylene-co-vinyl alcohol) coated 96-well microplates. Author Affiliation: (1) Department of Materials Science and Engineering, I-Shou University, Kaohsiung, 84001, Taiwan (2) Department of Physics and Astronomy, University of New Mexico, Albuquerque, NM, 87131, USA (3) Department of Chemical and Materials Engineering, National University of Kaohsiung (NUK), 700, Kaohsiung University Rd., Nan-Tzu District, Kaohsiung, 81148, Taiwan Article History: Registration Date: 09/04/2013 Received Date: 21/10/2012 Accepted Date: 09/04/2013 Online Date: 30/04/2013
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abstractByline: Mei-Hwa Lee (1), James L. Thomas (2), Yun-Chao Chen (3), Wei-Ti Chin (1), Hung-Yin Lin (3) Keywords: Molecular imprinting; Sandwich fluoroimmunoassays; Salivary proteins; Poly(ethylene-co-vinyl alcohol) Abstract: The replacement of antibodies by molecularly imprinted polymers (MIPs) has been investigated for many decades. However, indirect protocols (including natural primary and secondary antibodies) are still utilized to evaluate the ability of MIP thin films to recognize target molecules. MIPs can be prepared as either a thin film or as particles, and cavities that are complementary to the template can be generated on their surfaces. We have prepared thin film MIPs and particle MIPs prepared by solvent evaporation and phase inversion, respectively, from solutions of poly(ethylene-co-vinyl alcohol) (pEVAL) in the presence of the target analytes amylase, lysozyme, and lipase. These were first adsorbed on MIP thin films and by MIP particles that contain fluorescent quantum dots. Sandwich fluoroimmunoassays were then conducted to quantify them in MIP-coated 96-well microplates. The method was applied to determine amylase in saliva, and results were compared with a commercial analytical system. Figure The recognition of amylase-imprinted poly(ethylene-co-vinyl alcohol)/quantum dots composite nanoparticles to amylase on the amylase-imprinted poly(ethylene-co-vinyl alcohol) coated 96-well microplates. Author Affiliation: (1) Department of Materials Science and Engineering, I-Shou University, Kaohsiung, 84001, Taiwan (2) Department of Physics and Astronomy, University of New Mexico, Albuquerque, NM, 87131, USA (3) Department of Chemical and Materials Engineering, National University of Kaohsiung (NUK), 700, Kaohsiung University Rd., Nan-Tzu District, Kaohsiung, 81148, Taiwan Article History: Registration Date: 09/04/2013 Received Date: 21/10/2012 Accepted Date: 09/04/2013 Online Date: 30/04/2013
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