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DNA-templated synthesis of PtAu bimetallic nanoparticle/graphene nanocomposites and their application in glucose biosensor.(Report)(Author abstract)

Byline: Jing Leng (4), Wen-Min Wang (4), Li-Min Lu (4), Ling Bai (4), Xin-Lan Qiu (4) Keywords: Graphene; PtAu bimetallic nanoparticles; Glucose oxidase; Biosensor; Glucose Abstract: In this paper, single-stranded DNA (ss-DNA) is demonstrated to functionalize graphene (GR) and to further guide the g... Full description

Journal Title: Nanoscale Research Letters Dec, 2014, Vol.9(1), p.1(8)
Main Author: Leng, Jing
Other Authors: Wang, Wen - Min , Lu, Li - Min , Bai, Ling , Qiu, Xin - Lan
Format: Electronic Article Electronic Article
Language: English
Subjects:
Dna
ID: ISSN: 1931-7573
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recordid: gale_ofa360781269
title: DNA-templated synthesis of PtAu bimetallic nanoparticle/graphene nanocomposites and their application in glucose biosensor.(Report)(Author abstract)
format: Article
creator:
  • Leng, Jing
  • Wang, Wen - Min
  • Lu, Li - Min
  • Bai, Ling
  • Qiu, Xin - Lan
subjects:
  • Oxidases
  • Graphene
  • Glucose Metabolism
  • Electron Microscopy
  • Detection Equipment
  • Nanotechnology
  • Electrochemistry
  • Glucose
  • Dna
ispartof: Nanoscale Research Letters, Dec, 2014, Vol.9(1), p.1(8)
description: Byline: Jing Leng (4), Wen-Min Wang (4), Li-Min Lu (4), Ling Bai (4), Xin-Lan Qiu (4) Keywords: Graphene; PtAu bimetallic nanoparticles; Glucose oxidase; Biosensor; Glucose Abstract: In this paper, single-stranded DNA (ss-DNA) is demonstrated to functionalize graphene (GR) and to further guide the growth of PtAu bimetallic nanoparticles (PtAuNPs) on GR with high densities and dispersion. The obtained nanocomposites (PtAuNPs/ss-DNA/GR) were characterized by transmission electron microscopy (TEM), energy-dispersive X-ray spectrometer (EDS), and electrochemical techniques. Then, an enzyme nanoassembly was prepared by self-assembling glucose oxidase (GOD) on PtAuNP/ss-DNA/GR nanocomposites (GOD/PtAuNPs/ss-DNA/GR). The nanocomposites provided a suitable microenvironment for GOD to retain its biological activity. The direct and reversible electron transfer process between the active site of GOD and the modified electrode was realized without any extra electron mediator. Thus, the prepared GOD/PtAuNP/ss-DNA/GR electrode was proposed as a biosensor for the quantification of glucose. The effects of pH, applied potential, and temperature on the performance of the biosensor were discussed in detail and were optimized. Under optimal conditions, the biosensor showed a linearity with glucose concentration in the range of 1.0 to 1,800 uM with a detection limit of 0.3 uM (S/N=3). The results demonstrate that the developed approach provides a promising strategy to improve the sensitivity and enzyme activity of electrochemical biosensors. Author Affiliation: (4) College of Science, Jiangxi Agricultural University, Nanchang, 330045, People's Republic of China Article History: Registration Date: 26/11/2013 Received Date: 26/11/2013 Accepted Date: 19/01/2014 Online Date: 27/02/2014
language: English
source:
identifier: ISSN: 1931-7573
fulltext: fulltext
issn:
  • 1931-7573
  • 19317573
url: Link


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titleDNA-templated synthesis of PtAu bimetallic nanoparticle/graphene nanocomposites and their application in glucose biosensor.(Report)(Author abstract)
creatorLeng, Jing ; Wang, Wen - Min ; Lu, Li - Min ; Bai, Ling ; Qiu, Xin - Lan
ispartofNanoscale Research Letters, Dec, 2014, Vol.9(1), p.1(8)
identifierISSN: 1931-7573
subjectOxidases ; Graphene ; Glucose Metabolism ; Electron Microscopy ; Detection Equipment ; Nanotechnology ; Electrochemistry ; Glucose ; Dna
descriptionByline: Jing Leng (4), Wen-Min Wang (4), Li-Min Lu (4), Ling Bai (4), Xin-Lan Qiu (4) Keywords: Graphene; PtAu bimetallic nanoparticles; Glucose oxidase; Biosensor; Glucose Abstract: In this paper, single-stranded DNA (ss-DNA) is demonstrated to functionalize graphene (GR) and to further guide the growth of PtAu bimetallic nanoparticles (PtAuNPs) on GR with high densities and dispersion. The obtained nanocomposites (PtAuNPs/ss-DNA/GR) were characterized by transmission electron microscopy (TEM), energy-dispersive X-ray spectrometer (EDS), and electrochemical techniques. Then, an enzyme nanoassembly was prepared by self-assembling glucose oxidase (GOD) on PtAuNP/ss-DNA/GR nanocomposites (GOD/PtAuNPs/ss-DNA/GR). The nanocomposites provided a suitable microenvironment for GOD to retain its biological activity. The direct and reversible electron transfer process between the active site of GOD and the modified electrode was realized without any extra electron mediator. Thus, the prepared GOD/PtAuNP/ss-DNA/GR electrode was proposed as a biosensor for the quantification of glucose. The effects of pH, applied potential, and temperature on the performance of the biosensor were discussed in detail and were optimized. Under optimal conditions, the biosensor showed a linearity with glucose concentration in the range of 1.0 to 1,800 uM with a detection limit of 0.3 uM (S/N=3). The results demonstrate that the developed approach provides a promising strategy to improve the sensitivity and enzyme activity of electrochemical biosensors. Author Affiliation: (4) College of Science, Jiangxi Agricultural University, Nanchang, 330045, People's Republic of China Article History: Registration Date: 26/11/2013 Received Date: 26/11/2013 Accepted Date: 19/01/2014 Online Date: 27/02/2014
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titleDNA-templated synthesis of PtAu bimetallic nanoparticle/graphene nanocomposites and their application in glucose biosensor.(Report)(Author abstract)
descriptionByline: Jing Leng (4), Wen-Min Wang (4), Li-Min Lu (4), Ling Bai (4), Xin-Lan Qiu (4) Keywords: Graphene; PtAu bimetallic nanoparticles; Glucose oxidase; Biosensor; Glucose Abstract: In this paper, single-stranded DNA (ss-DNA) is demonstrated to functionalize graphene (GR) and to further guide the growth of PtAu bimetallic nanoparticles (PtAuNPs) on GR with high densities and dispersion. The obtained nanocomposites (PtAuNPs/ss-DNA/GR) were characterized by transmission electron microscopy (TEM), energy-dispersive X-ray spectrometer (EDS), and electrochemical techniques. Then, an enzyme nanoassembly was prepared by self-assembling glucose oxidase (GOD) on PtAuNP/ss-DNA/GR nanocomposites (GOD/PtAuNPs/ss-DNA/GR). The nanocomposites provided a suitable microenvironment for GOD to retain its biological activity. The direct and reversible electron transfer process between the active site of GOD and the modified electrode was realized without any extra electron mediator. Thus, the prepared GOD/PtAuNP/ss-DNA/GR electrode was proposed as a biosensor for the quantification of glucose. The effects of pH, applied potential, and temperature on the performance of the biosensor were discussed in detail and were optimized. Under optimal conditions, the biosensor showed a linearity with glucose concentration in the range of 1.0 to 1,800 uM with a detection limit of 0.3 uM (S/N=3). The results demonstrate that the developed approach provides a promising strategy to improve the sensitivity and enzyme activity of electrochemical biosensors. Author Affiliation: (4) College of Science, Jiangxi Agricultural University, Nanchang, 330045, People's Republic of China Article History: Registration Date: 26/11/2013 Received Date: 26/11/2013 Accepted Date: 19/01/2014 Online Date: 27/02/2014
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abstractByline: Jing Leng (4), Wen-Min Wang (4), Li-Min Lu (4), Ling Bai (4), Xin-Lan Qiu (4) Keywords: Graphene; PtAu bimetallic nanoparticles; Glucose oxidase; Biosensor; Glucose Abstract: In this paper, single-stranded DNA (ss-DNA) is demonstrated to functionalize graphene (GR) and to further guide the growth of PtAu bimetallic nanoparticles (PtAuNPs) on GR with high densities and dispersion. The obtained nanocomposites (PtAuNPs/ss-DNA/GR) were characterized by transmission electron microscopy (TEM), energy-dispersive X-ray spectrometer (EDS), and electrochemical techniques. Then, an enzyme nanoassembly was prepared by self-assembling glucose oxidase (GOD) on PtAuNP/ss-DNA/GR nanocomposites (GOD/PtAuNPs/ss-DNA/GR). The nanocomposites provided a suitable microenvironment for GOD to retain its biological activity. The direct and reversible electron transfer process between the active site of GOD and the modified electrode was realized without any extra electron mediator. Thus, the prepared GOD/PtAuNP/ss-DNA/GR electrode was proposed as a biosensor for the quantification of glucose. The effects of pH, applied potential, and temperature on the performance of the biosensor were discussed in detail and were optimized. Under optimal conditions, the biosensor showed a linearity with glucose concentration in the range of 1.0 to 1,800 uM with a detection limit of 0.3 uM (S/N=3). The results demonstrate that the developed approach provides a promising strategy to improve the sensitivity and enzyme activity of electrochemical biosensors. Author Affiliation: (4) College of Science, Jiangxi Agricultural University, Nanchang, 330045, People's Republic of China Article History: Registration Date: 26/11/2013 Received Date: 26/11/2013 Accepted Date: 19/01/2014 Online Date: 27/02/2014
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