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Extending single-molecule microscopy using optical fourier processing.(Report)

This article surveys the recent application of optical Fourier processing to the long-established but still expanding field of single-molecule imaging and microscopy. A variety of single-molecule studies can benefit from the additional image information that can be obtained by modulating the Fourier... Full description

Journal Title: Journal of Physical Chemistry B July 17, 2014, Vol.118(28), p.8313-8329
Main Author: Backer, Adam S.
Other Authors: Moerner, W. E.
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 1520-6106
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recordid: gale_ofa380696510
title: Extending single-molecule microscopy using optical fourier processing.(Report)
format: Article
creator:
  • Backer, Adam S.
  • Moerner, W. E.
subjects:
  • Computer Simulation -- Analysis
  • Fourier Analysis
ispartof: Journal of Physical Chemistry B, July 17, 2014, Vol.118(28), p.8313-8329
description: This article surveys the recent application of optical Fourier processing to the long-established but still expanding field of single-molecule imaging and microscopy. A variety of single-molecule studies can benefit from the additional image information that can be obtained by modulating the Fourier, or pupil, plane of a widefield microscope. After briefly reviewing several current applications, we present a comprehensive and computationally efficient theoretical model for simulating single-molecule fluorescence as it propagates through an imaging system. Furthermore, we describe how phase/amplitude-modulating optics inserted in the imaging pathway may be modeled, especially at the Fourier plane. Finally, we discuss selected recent applications of Fourier processing methods to measure the orientation, depth, and rotational mobility of single fluorescent molecules.
language: English
source:
identifier: ISSN: 1520-6106
fulltext: fulltext
issn:
  • 1520-6106
  • 15206106
url: Link


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titleExtending single-molecule microscopy using optical fourier processing.(Report)
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descriptionThis article surveys the recent application of optical Fourier processing to the long-established but still expanding field of single-molecule imaging and microscopy. A variety of single-molecule studies can benefit from the additional image information that can be obtained by modulating the Fourier, or pupil, plane of a widefield microscope. After briefly reviewing several current applications, we present a comprehensive and computationally efficient theoretical model for simulating single-molecule fluorescence as it propagates through an imaging system. Furthermore, we describe how phase/amplitude-modulating optics inserted in the imaging pathway may be modeled, especially at the Fourier plane. Finally, we discuss selected recent applications of Fourier processing methods to measure the orientation, depth, and rotational mobility of single fluorescent molecules.
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