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Development of a fully automated toxicological LC-M[S.sup.n] screening system in urine using online extraction with turbulent flow chromatography.(ORIGINAL PAPER)

In clinical toxicology, fast and specific methods are necessary for the screening of different classes of drugs. Therefore, an online extraction high-performance liquid chromatography coupled to mass spectrometry (LC-M[S.sup.n]) screening method using a MS2 and M[S.sup.3] spectral library for the id... Full description

Journal Title: Analytical and Bioanalytical Chemistry April 15, 2011, Vol.400(1), p.89(12)
Main Author: Mueller, Daniel M.
Other Authors: Duretz, Benedicte , Espourteille, Francois A. , Rentsch, Katharina M.
Format: Electronic Article Electronic Article
Language: English
Subjects:
Quelle: Cengage Learning, Inc.
ID: ISSN: 1618-2642 ; DOI: 10.1007/s00216-010-4560-4
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title: Development of a fully automated toxicological LC-M[S.sup.n] screening system in urine using online extraction with turbulent flow chromatography.(ORIGINAL PAPER)
format: Article
creator:
  • Mueller, Daniel M.
  • Duretz, Benedicte
  • Espourteille, Francois A.
  • Rentsch, Katharina M.
subjects:
  • Enzymes – Analysis
  • High Performance Liquid Chromatography – Analysis
  • Hydrolysis – Analysis
ispartof: Analytical and Bioanalytical Chemistry, April 15, 2011, Vol.400(1), p.89(12)
description: In clinical toxicology, fast and specific methods are necessary for the screening of different classes of drugs. Therefore, an online extraction high-performance liquid chromatography coupled to mass spectrometry (LC-M[S.sup.n]) screening method using a MS2 and M[S.sup.3] spectral library for the identification of xenobiotic substances has been developed and validated. Samples were run twice, once native and once after enzymatic hydrolysis. Internal standards and buffer were added to the urine samples. Following centrifugation, the supernatant was injected into the system. Extraction was performed by online turbulent flow chromatography. The chromatographic separation was achieved using a Phenyl/Hexyl column. For detection, a linear ion trap, equipped with an APCI interface, was used and the different compounds were identified using a M[S.sup.2] and M[S.sup.3] spectral library containing 356 compounds. The turnaround time to report the results of the screening including hydrolysis was approximately 2 h. About 92% of the 356 substances could be identified with a limit of identification below 100 ng/ml. The recovery and matrix effect experiments showed suitable results, and in six drug-free urine samples of healthy volunteers analyzed for selectivity, no substances have been identified. Carryover could be well controlled, and the method had a good reproducibility. The comparison of the results of 103 real patient urine samples showed a good agreement between the existing GC-MS and LC-MS methods with offline extraction and the new online extraction LC-M[S.sup.n] screening method. The presented method allows a fast and sensitive analysis of a broad range of compounds. Keywords LC-MS * Turbulent flow chromatography * Online extraction * Toxicological screening * Clinical toxicology
language: eng
source: Cengage Learning, Inc.
identifier: ISSN: 1618-2642 ; DOI: 10.1007/s00216-010-4560-4
fulltext: fulltext
issn:
  • 1618-2642
  • 16182642
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titleDevelopment of a fully automated toxicological LC-M[S.sup.n] screening system in urine using online extraction with turbulent flow chromatography.(ORIGINAL PAPER)
creatorMueller, Daniel M. ; Duretz, Benedicte ; Espourteille, Francois A. ; Rentsch, Katharina M.
ispartofAnalytical and Bioanalytical Chemistry, April 15, 2011, Vol.400(1), p.89(12)
identifierISSN: 1618-2642 ; DOI: 10.1007/s00216-010-4560-4
subjectEnzymes – Analysis ; High Performance Liquid Chromatography – Analysis ; Hydrolysis – Analysis
descriptionIn clinical toxicology, fast and specific methods are necessary for the screening of different classes of drugs. Therefore, an online extraction high-performance liquid chromatography coupled to mass spectrometry (LC-M[S.sup.n]) screening method using a MS2 and M[S.sup.3] spectral library for the identification of xenobiotic substances has been developed and validated. Samples were run twice, once native and once after enzymatic hydrolysis. Internal standards and buffer were added to the urine samples. Following centrifugation, the supernatant was injected into the system. Extraction was performed by online turbulent flow chromatography. The chromatographic separation was achieved using a Phenyl/Hexyl column. For detection, a linear ion trap, equipped with an APCI interface, was used and the different compounds were identified using a M[S.sup.2] and M[S.sup.3] spectral library containing 356 compounds. The turnaround time to report the results of the screening including hydrolysis was approximately 2 h. About 92% of the 356 substances could be identified with a limit of identification below 100 ng/ml. The recovery and matrix effect experiments showed suitable results, and in six drug-free urine samples of healthy volunteers analyzed for selectivity, no substances have been identified. Carryover could be well controlled, and the method had a good reproducibility. The comparison of the results of 103 real patient urine samples showed a good agreement between the existing GC-MS and LC-MS methods with offline extraction and the new online extraction LC-M[S.sup.n] screening method. The presented method allows a fast and sensitive analysis of a broad range of compounds. Keywords LC-MS * Turbulent flow chromatography * Online extraction * Toxicological screening * Clinical toxicology
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titleDevelopment of a fully automated toxicological LC-M[S.sup.n] screening system in urine using online extraction with turbulent flow chromatography.(ORIGINAL PAPER)
descriptionIn clinical toxicology, fast and specific methods are necessary for the screening of different classes of drugs. Therefore, an online extraction high-performance liquid chromatography coupled to mass spectrometry (LC-M[S.sup.n]) screening method using a MS2 and M[S.sup.3] spectral library for the identification of xenobiotic substances has been developed and validated. Samples were run twice, once native and once after enzymatic hydrolysis. Internal standards and buffer were added to the urine samples. Following centrifugation, the supernatant was injected into the system. Extraction was performed by online turbulent flow chromatography. The chromatographic separation was achieved using a Phenyl/Hexyl column. For detection, a linear ion trap, equipped with an APCI interface, was used and the different compounds were identified using a M[S.sup.2] and M[S.sup.3] spectral library containing 356 compounds. The turnaround time to report the results of the screening including hydrolysis was approximately 2 h. About 92% of the 356 substances could be identified with a limit of identification below 100 ng/ml. The recovery and matrix effect experiments showed suitable results, and in six drug-free urine samples of healthy volunteers analyzed for selectivity, no substances have been identified. Carryover could be well controlled, and the method had a good reproducibility. The comparison of the results of 103 real patient urine samples showed a good agreement between the existing GC-MS and LC-MS methods with offline extraction and the new online extraction LC-M[S.sup.n] screening method. The presented method allows a fast and sensitive analysis of a broad range of compounds. Keywords LC-MS * Turbulent flow chromatography * Online extraction * Toxicological screening * Clinical toxicology
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abstractIn clinical toxicology, fast and specific methods are necessary for the screening of different classes of drugs. Therefore, an online extraction high-performance liquid chromatography coupled to mass spectrometry (LC-M[S.sup.n]) screening method using a MS2 and M[S.sup.3] spectral library for the identification of xenobiotic substances has been developed and validated. Samples were run twice, once native and once after enzymatic hydrolysis. Internal standards and buffer were added to the urine samples. Following centrifugation, the supernatant was injected into the system. Extraction was performed by online turbulent flow chromatography. The chromatographic separation was achieved using a Phenyl/Hexyl column. For detection, a linear ion trap, equipped with an APCI interface, was used and the different compounds were identified using a M[S.sup.2] and M[S.sup.3] spectral library containing 356 compounds. The turnaround time to report the results of the screening including hydrolysis was approximately 2 h. About 92% of the 356 substances could be identified with a limit of identification below 100 ng/ml. The recovery and matrix effect experiments showed suitable results, and in six drug-free urine samples of healthy volunteers analyzed for selectivity, no substances have been identified. Carryover could be well controlled, and the method had a good reproducibility. The comparison of the results of 103 real patient urine samples showed a good agreement between the existing GC-MS and LC-MS methods with offline extraction and the new online extraction LC-M[S.sup.n] screening method. The presented method allows a fast and sensitive analysis of a broad range of compounds. Keywords LC-MS * Turbulent flow chromatography * Online extraction * Toxicological screening * Clinical toxicology
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