schliessen

Filtern

 

Bibliotheken

Characterisation of Skin Barrier Function Using Bioengineering and Biophysical Techniques.(Report)

Byline: Quan Yang (1,2), Richard H. Guy (1) Keywords: infrared spectroscopy; reverse iontophoresis; skin barrier function natural moisturizing factor (NMF); stratum corneum Abstract: Purpose To characterise skin barrier function in vivo at two distinct anatomic sites using minimally invasive bioengi... Full description

Journal Title: Pharmaceutical Research 2015, Vol.32(2), p.445(13)
Main Author: Yang, Quan
Other Authors: Guy, Richard H.
Format: Electronic Article Electronic Article
Language: English
Subjects:
Quelle: Cengage Learning, Inc.
ID: ISSN: 0724-8741
Zum Text:
SendSend as email Add to Book BagAdd to Book Bag
Staff View
recordid: gale_ofa398274864
title: Characterisation of Skin Barrier Function Using Bioengineering and Biophysical Techniques.(Report)
format: Article
creator:
  • Yang, Quan
  • Guy, Richard H.
subjects:
  • Mass Spectrometry – Methods
  • Pharmacy – Methods
  • Skin – Methods
  • Lipids – Methods
  • Liquid Chromatography – Methods
  • Bioengineering – Methods
  • Drugstores – Methods
ispartof: Pharmaceutical Research, 2015, Vol.32(2), p.445(13)
description: Byline: Quan Yang (1,2), Richard H. Guy (1) Keywords: infrared spectroscopy; reverse iontophoresis; skin barrier function natural moisturizing factor (NMF); stratum corneum Abstract: Purpose To characterise skin barrier function in vivo at two distinct anatomic sites using minimally invasive bioengineering and biophysical tools. Methods In healthy human volunteers, the quantities of stratum corneum (SC) per unit area of skin on the forearm and forehead were quantified by gravimetric and imaging techniques. Organisation of the SC intercellular lipids was evaluated as a function of position using attenuated total reflectance infrared spectroscopy (ATR-IR). The constituents of natural moisturising factor (NMF) were extracted from tape-stripped samples of the SC and by reverse iontophoresis 21 components were identified and quantified by liquid chromatography with mass spectrometric detection. Results SC was quantified more accurately by imaging and was significantly thinner on the forehead than on the forearm. Intercellular lipids were more disordered near the skin surface at both sites however, throughout forearm SC, the lipids were substantially better organised than those in the forehead. Compositionally, the NMF from forearm and forehead SC was similar, but the total amount extractable from the forehead was smaller. Conclusion Taken together, the bioengineering and biophysical techniques employed demonstrate, in a complementary, objective and quantitative fashion, that SC barrier function on the forehead is less competent than that on the forearm. Author Affiliation: (1) Department of Pharmacy & Pharmacology, University of Bath, Claverton Down, Bath, BA2 7AY, UK (2) MHRA, 151 Buckingham Palace Road, London, SW1W 9SZ, UK Article History: Registration Date: 25/07/2014 Received Date: 25/02/2014 Accepted Date: 24/07/2014 Online Date: 05/08/2014
language: eng
source: Cengage Learning, Inc.
identifier: ISSN: 0724-8741
fulltext: fulltext
issn:
  • 0724-8741
  • 07248741
url: Link


@attributes
ID219086592
RANK0.07
NO1
SEARCH_ENGINEprimo_central_multiple_fe
SEARCH_ENGINE_TYPEPrimo Central Search Engine
LOCALfalse
PrimoNMBib
record
control
sourcerecordid398274864
sourceidgale_ofa
recordidTN_gale_ofa398274864
sourceformatXML
sourcesystemPC
galeid398274864
display
typearticle
titleCharacterisation of Skin Barrier Function Using Bioengineering and Biophysical Techniques.(Report)
creatorYang, Quan ; Guy, Richard H.
ispartofPharmaceutical Research, 2015, Vol.32(2), p.445(13)
identifierISSN: 0724-8741
subjectMass Spectrometry – Methods ; Pharmacy – Methods ; Skin – Methods ; Lipids – Methods ; Liquid Chromatography – Methods ; Bioengineering – Methods ; Drugstores – Methods
descriptionByline: Quan Yang (1,2), Richard H. Guy (1) Keywords: infrared spectroscopy; reverse iontophoresis; skin barrier function natural moisturizing factor (NMF); stratum corneum Abstract: Purpose To characterise skin barrier function in vivo at two distinct anatomic sites using minimally invasive bioengineering and biophysical tools. Methods In healthy human volunteers, the quantities of stratum corneum (SC) per unit area of skin on the forearm and forehead were quantified by gravimetric and imaging techniques. Organisation of the SC intercellular lipids was evaluated as a function of position using attenuated total reflectance infrared spectroscopy (ATR-IR). The constituents of natural moisturising factor (NMF) were extracted from tape-stripped samples of the SC and by reverse iontophoresis 21 components were identified and quantified by liquid chromatography with mass spectrometric detection. Results SC was quantified more accurately by imaging and was significantly thinner on the forehead than on the forearm. Intercellular lipids were more disordered near the skin surface at both sites however, throughout forearm SC, the lipids were substantially better organised than those in the forehead. Compositionally, the NMF from forearm and forehead SC was similar, but the total amount extractable from the forehead was smaller. Conclusion Taken together, the bioengineering and biophysical techniques employed demonstrate, in a complementary, objective and quantitative fashion, that SC barrier function on the forehead is less competent than that on the forearm. Author Affiliation: (1) Department of Pharmacy & Pharmacology, University of Bath, Claverton Down, Bath, BA2 7AY, UK (2) MHRA, 151 Buckingham Palace Road, London, SW1W 9SZ, UK Article History: Registration Date: 25/07/2014 Received Date: 25/02/2014 Accepted Date: 24/07/2014 Online Date: 05/08/2014
languageeng
sourceCengage Learning, Inc.
lds50peer_reviewed
links
openurl$$Topenurl_article
openurlfulltext$$Topenurlfull_article
search
scope
0gale_onefilea
1OneFile
creatorcontrib
0Yang, Quan
1Quan Yang
2Guy, Richard H
titleCharacterisation of Skin Barrier Function Using Bioengineering and Biophysical Techniques.(Report)
descriptionByline: Quan Yang (1,2), Richard H. Guy (1) Keywords: infrared spectroscopy; reverse iontophoresis; skin barrier function natural moisturizing factor (NMF); stratum corneum Abstract: Purpose To characterise skin barrier function in vivo at two distinct anatomic sites using minimally invasive bioengineering and biophysical tools. Methods In healthy human volunteers, the quantities of stratum corneum (SC) per unit area of skin on the forearm and forehead were quantified by gravimetric and imaging techniques. Organisation of the SC intercellular lipids was evaluated as a function of position using attenuated total reflectance infrared spectroscopy (ATR-IR). The constituents of natural moisturising factor (NMF) were extracted from tape-stripped samples of the SC and by reverse iontophoresis 21 components were identified and quantified by liquid chromatography with mass spectrometric detection. Results SC was quantified more accurately by imaging and was significantly thinner on the forehead than on the forearm. Intercellular lipids were more disordered near the skin surface at both sites however, throughout forearm SC, the lipids were substantially better organised than those in the forehead. Compositionally, the NMF from forearm and forehead SC was similar, but the total amount extractable from the forehead was smaller. Conclusion Taken together, the bioengineering and biophysical techniques employed demonstrate, in a complementary, objective and quantitative fashion, that SC barrier function on the forehead is less competent than that on the forearm. Author Affiliation: (1) Department of Pharmacy & Pharmacology, University of Bath, Claverton Down, Bath, BA2 7AY, UK (2) MHRA, 151 Buckingham Palace Road, London, SW1W 9SZ, UK Article History: Registration Date: 25/07/2014 Received Date: 25/02/2014 Accepted Date: 24/07/2014 Online Date: 05/08/2014
subject
0Mass spectrometry–Methods
1Pharmacy–Methods
2Skin–Methods
3Lipids–Methods
4Liquid chromatography–Methods
5Bioengineering–Methods
6Drugstores–Methods
75912
8Drug stores and proprietary stores
9Analytical Laboratory Instrument Manufacturing
10Pharmacies and Drug Stores
11334516
12446110
general
0English
1Springer
2Cengage Learning, Inc.
sourceidgale_ofa
recordidgale_ofa398274864
issn
00724-8741
107248741
rsrctypearticle
creationdate2015
startdate20150201
recordtypearticle
enddate20150201
addtitlePharmaceutical Research
searchscopeOneFile
citationpf 445 vol 32 issue 2
sort
titleCharacterisation of Skin Barrier Function Using Bioengineering and Biophysical Techniques.(Report)
authorYang, Quan ; Guy, Richard H.
creationdate20150201
lso0120150201
facets
frbrgroupid12884018507667961
frbrtype6
languageeng
creationdate2015
topic
0Mass Spectrometry–Methods
1Pharmacy–Methods
2Skin–Methods
3Lipids–Methods
4Liquid Chromatography–Methods
5Bioengineering–Methods
6Drugstores–Methods
collectionOneFile (GALE)
prefilterarticles
rsrctypearticles
creatorcontrib
0Yang, Quan
1Guy, Richard H.
jtitlePharmaceutical Research
toplevelpeer_reviewed
delivery
delcategoryRemote Search Resource
fulltextfulltext
addata
au
0Yang, Quan
1Guy, Richard H.
spage445
formatjournal
genrearticle
ristypeJOUR
abstractByline: Quan Yang (1,2), Richard H. Guy (1) Keywords: infrared spectroscopy; reverse iontophoresis; skin barrier function natural moisturizing factor (NMF); stratum corneum Abstract: Purpose To characterise skin barrier function in vivo at two distinct anatomic sites using minimally invasive bioengineering and biophysical tools. Methods In healthy human volunteers, the quantities of stratum corneum (SC) per unit area of skin on the forearm and forehead were quantified by gravimetric and imaging techniques. Organisation of the SC intercellular lipids was evaluated as a function of position using attenuated total reflectance infrared spectroscopy (ATR-IR). The constituents of natural moisturising factor (NMF) were extracted from tape-stripped samples of the SC and by reverse iontophoresis 21 components were identified and quantified by liquid chromatography with mass spectrometric detection. Results SC was quantified more accurately by imaging and was significantly thinner on the forehead than on the forearm. Intercellular lipids were more disordered near the skin surface at both sites however, throughout forearm SC, the lipids were substantially better organised than those in the forehead. Compositionally, the NMF from forearm and forehead SC was similar, but the total amount extractable from the forehead was smaller. Conclusion Taken together, the bioengineering and biophysical techniques employed demonstrate, in a complementary, objective and quantitative fashion, that SC barrier function on the forehead is less competent than that on the forearm. Author Affiliation: (1) Department of Pharmacy & Pharmacology, University of Bath, Claverton Down, Bath, BA2 7AY, UK (2) MHRA, 151 Buckingham Palace Road, London, SW1W 9SZ, UK Article History: Registration Date: 25/07/2014 Received Date: 25/02/2014 Accepted Date: 24/07/2014 Online Date: 05/08/2014
lad01gale_ofa
aulast
0Yang
1Guy
aufirst
0Quan
1Richard H.
atitleCharacterisation of Skin Barrier Function Using Bioengineering and Biophysical Techniques.
volume32
issue2
issn0724-8741
pubSpringer
date2015-02-01