schliessen

Filtern

 

Bibliotheken

The Impact of Various Time Intervals on the Supragingival Plaque Dynamic Core Microbiome

Objective The aim of this study was to examine the influence of various time intervals on the composition of the supragingival plaque microbiome, especially the dynamic core microbiome, and to find a suitable observation interval for further studies on oral microbiota. Methods and Materials Eight qu... Full description

Journal Title: PLoS ONE May 5, 2015, Vol.10(5)
Main Author: Jiang, Wen-Xin
Other Authors: Hu, Yue-Jian , Gao, Li , He, Zhi-Yan , Zhu, Cai-Lian , Ma, Rui , Huang, Zheng-Wei
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 1932-6203
Zum Text:
SendSend as email Add to Book BagAdd to Book Bag
Staff View
recordid: gale_ofa430193537
title: The Impact of Various Time Intervals on the Supragingival Plaque Dynamic Core Microbiome
format: Article
creator:
  • Jiang, Wen-Xin
  • Hu, Yue-Jian
  • Gao, Li
  • He, Zhi-Yan
  • Zhu, Cai-Lian
  • Ma, Rui
  • Huang, Zheng-Wei
subjects:
  • Microbiota (Symbiotic Organisms) – Analysis
ispartof: PLoS ONE, May 5, 2015, Vol.10(5)
description: Objective The aim of this study was to examine the influence of various time intervals on the composition of the supragingival plaque microbiome, especially the dynamic core microbiome, and to find a suitable observation interval for further studies on oral microbiota. Methods and Materials Eight qualified volunteers whose respective age ranges from 25 to 28 years participated in the present study. The supragingival plaque was collected from the buccogingival surface of the maxillary first molar at eight time slots with different intervals (day 0, 1 day, 3 days, 1 week, 2 weeks, 3 weeks, 1 month, and 3 months). Bioinformatic analyses was performed based on 16S rDNA pyrosequencing (454 sequencing platform) targeting at the hypervariable V4-V5 region, in order to assess the diversity and variation of the supragingival plaque microbiome. Results A total of 359,565 qualified reads for 64 samples were generated for subsequent analyses, which represents 8,452 operational taxonomic units identified at 3% dissimilarity. The dynamic core microbiome detected in the current study included five phyla, 12 genera and 13 species. At the genus level, the relative abundance of bacterial communities under the "1 day," "1 month," and "3 months" intervals was clustered into sub-category. At the species level, the number of overlapping species remained stable between the "1 month" and "3 months" intervals, whereas the number of dynamic core species became stable within only 1 week. Conclusions This study emphasized the impact of different time intervals (days, weeks and months) on the composition, commonality and diversity of the supragingival microbiome. The analyses found that for various types of studies, the time interval of a month is more suitable for observing the general composition of the supragingival microbiome, and that a week is better for observing the dynamic core microbiome.
language: eng
source:
identifier: ISSN: 1932-6203
fulltext: fulltext
issn:
  • 1932-6203
  • 19326203
url: Link


@attributes
ID950459220
RANK0.07
NO1
SEARCH_ENGINEprimo_central_multiple_fe
SEARCH_ENGINE_TYPEPrimo Central Search Engine
LOCALfalse
PrimoNMBib
record
control
sourcerecordid430193537
sourceidgale_ofa
recordidTN_gale_ofa430193537
sourceformatXML
sourcesystemOther
pqid1680187553
galeid430193537
display
typearticle
titleThe Impact of Various Time Intervals on the Supragingival Plaque Dynamic Core Microbiome
creatorJiang, Wen-Xin ; Hu, Yue-Jian ; Gao, Li ; He, Zhi-Yan ; Zhu, Cai-Lian ; Ma, Rui ; Huang, Zheng-Wei
ispartofPLoS ONE, May 5, 2015, Vol.10(5)
identifierISSN: 1932-6203
subjectMicrobiota (Symbiotic Organisms) – Analysis
descriptionObjective The aim of this study was to examine the influence of various time intervals on the composition of the supragingival plaque microbiome, especially the dynamic core microbiome, and to find a suitable observation interval for further studies on oral microbiota. Methods and Materials Eight qualified volunteers whose respective age ranges from 25 to 28 years participated in the present study. The supragingival plaque was collected from the buccogingival surface of the maxillary first molar at eight time slots with different intervals (day 0, 1 day, 3 days, 1 week, 2 weeks, 3 weeks, 1 month, and 3 months). Bioinformatic analyses was performed based on 16S rDNA pyrosequencing (454 sequencing platform) targeting at the hypervariable V4-V5 region, in order to assess the diversity and variation of the supragingival plaque microbiome. Results A total of 359,565 qualified reads for 64 samples were generated for subsequent analyses, which represents 8,452 operational taxonomic units identified at 3% dissimilarity. The dynamic core microbiome detected in the current study included five phyla, 12 genera and 13 species. At the genus level, the relative abundance of bacterial communities under the "1 day," "1 month," and "3 months" intervals was clustered into sub-category. At the species level, the number of overlapping species remained stable between the "1 month" and "3 months" intervals, whereas the number of dynamic core species became stable within only 1 week. Conclusions This study emphasized the impact of different time intervals (days, weeks and months) on the composition, commonality and diversity of the supragingival microbiome. The analyses found that for various types of studies, the time interval of a month is more suitable for observing the general composition of the supragingival microbiome, and that a week is better for observing the dynamic core microbiome.
languageeng
source
version9
lds50peer_reviewed
links
openurl$$Topenurl_article
openurlfulltext$$Topenurlfull_article
search
scope
0gale_onefilea
1OneFile
creatorcontrib
0Jiang, Wen-xin
1Wen-xin Jiang; Yue-jian Hu; Li Gao; Zhi-yan He; Cai-lian Zhu; Rui Ma; Zheng-wei Huang
2Hu, Yue-jian
3Gao, Li
4He, Zhi-yan
5Zhu, Cai-lian
6Ma, Rui
7Huang, Zheng-wei
titleThe Impact of Various Time Intervals on the Supragingival Plaque Dynamic Core Microbiome.
descriptionObjective The aim of this study was to examine the influence of various time intervals on the composition of the supragingival plaque microbiome, especially the dynamic core microbiome, and to find a suitable observation interval for further studies on oral microbiota. Methods and Materials Eight qualified volunteers whose respective age ranges from 25 to 28 years participated in the present study. The supragingival plaque was collected from the buccogingival surface of the maxillary first molar at eight time slots with different intervals (day 0, 1 day, 3 days, 1 week, 2 weeks, 3 weeks, 1 month, and 3 months). Bioinformatic analyses was performed based on 16S rDNA pyrosequencing (454 sequencing platform) targeting at the hypervariable V4-V5 region, in order to assess the diversity and variation of the supragingival plaque microbiome. Results A total of 359,565 qualified reads for 64 samples were generated for subsequent analyses, which represents 8,452 operational taxonomic units identified at 3% dissimilarity. The dynamic core microbiome detected in the current study included five phyla, 12 genera and 13 species. At the genus level, the relative abundance of bacterial communities under the "1 day," "1 month," and "3 months" intervals was clustered into sub-category. At the species level, the number of overlapping species remained stable between the "1 month" and "3 months" intervals, whereas the number of dynamic core species became stable within only 1 week. Conclusions This study emphasized the impact of different time intervals (days, weeks and months) on the composition, commonality and diversity of the supragingival microbiome. The analyses found that for various types of studies, the time interval of a month is more suitable for observing the general composition of the supragingival microbiome, and that a week is better for observing the dynamic core microbiome.
subjectMicrobiota (Symbiotic organisms)–Analysis
general
0English
1Public Library of Science
2Cengage Learning, Inc.
sourceidgale_ofa
recordidgale_ofa430193537
issn
01932-6203
119326203
rsrctypearticle
creationdate2015
startdate20150505
enddate20150505
recordtypearticle
addtitlePLoS ONE
searchscopeOneFile
citationvol 10 issue 5
lsr30VSR-Enriched:[pqid, pages, doi, galeid]
sort
titleThe Impact of Various Time Intervals on the Supragingival Plaque Dynamic Core Microbiome.
authorJiang, Wen-Xin ; Hu, Yue-Jian ; Gao, Li ; He, Zhi-Yan ; Zhu, Cai-Lian ; Ma, Rui ; Huang, Zheng-Wei
creationdate20150505
lso0120150505
facets
frbrgroupid1456159099577147532
frbrtype5
languageeng
creationdate2015
topicMicrobiota (Symbiotic Organisms)–Analysis
collectionOneFile (GALE)
prefilterarticles
rsrctypearticles
creatorcontrib
0Jiang, Wen-Xin
1Hu, Yue-Jian
2Gao, Li
3He, Zhi-Yan
4Zhu, Cai-Lian
5Ma, Rui
6Huang, Zheng-Wei
jtitlePLoS ONE
toplevelpeer_reviewed
delivery
delcategoryRemote Search Resource
fulltextfulltext
addata
aulast
0Jiang
1Hu
2Gao
3He
4Zhu
5Ma
6Huang
aufirst
0Wen-Xin
1Yue-Jian
2Li
3Zhi-Yan
4Cai-Lian
5Rui
6Zheng-Wei
au
0Jiang, Wen-xin
1Hu, Yue-jian
2Gao, Li
3He, Zhi-yan
4Zhu, Cai-lian
5Ma, Rui
6Huang, Zheng-wei
atitleThe Impact of Various Time Intervals on the Supragingival Plaque Dynamic Core Microbiome
jtitlePLoS ONE
risdate20150505
volume10
issue5
issn1932-6203
formatjournal
genrearticle
ristypeJOUR
abstractObjective The aim of this study was to examine the influence of various time intervals on the composition of the supragingival plaque microbiome, especially the dynamic core microbiome, and to find a suitable observation interval for further studies on oral microbiota. Methods and Materials Eight qualified volunteers whose respective age ranges from 25 to 28 years participated in the present study. The supragingival plaque was collected from the buccogingival surface of the maxillary first molar at eight time slots with different intervals (day 0, 1 day, 3 days, 1 week, 2 weeks, 3 weeks, 1 month, and 3 months). Bioinformatic analyses was performed based on 16S rDNA pyrosequencing (454 sequencing platform) targeting at the hypervariable V4-V5 region, in order to assess the diversity and variation of the supragingival plaque microbiome. Results A total of 359,565 qualified reads for 64 samples were generated for subsequent analyses, which represents 8,452 operational taxonomic units identified at 3% dissimilarity. The dynamic core microbiome detected in the current study included five phyla, 12 genera and 13 species. At the genus level, the relative abundance of bacterial communities under the "1 day," "1 month," and "3 months" intervals was clustered into sub-category. At the species level, the number of overlapping species remained stable between the "1 month" and "3 months" intervals, whereas the number of dynamic core species became stable within only 1 week. Conclusions This study emphasized the impact of different time intervals (days, weeks and months) on the composition, commonality and diversity of the supragingival microbiome. The analyses found that for various types of studies, the time interval of a month is more suitable for observing the general composition of the supragingival microbiome, and that a week is better for observing the dynamic core microbiome.
pubPublic Library of Science
lad01gale_ofa
pagese0124631
doi10.1371/journal.pone.0124631
date2015-05-05