schliessen

Filtern

 

Bibliotheken

The SND proteins constitute an alternative targeting route to the endoplasmic reticulum.(Report)

In eukaryotes, up to one-third of cellular proteins are targeted to the endoplasmic reticulum, where they undergo folding, processing, sorting and trafficking to subsequent endomembrane compartments. Targeting to the endoplasmic reticulum has been shown to occur co-translationally by the signal reco... Full description

Journal Title: Nature Dec 1, 2016, Vol.540(7631), p.134(5)
Main Author: Aviram, Naama
Other Authors: Ast, Tslil , Costa, Elizabeth A. , Arakel, Eric C. , Chuartzman, Silvia G. , Jan, Calvin H. , Hadenteufel, Sarah , Dudek, Johanna , Jung, Martin , Schorr, Stefan , Zimmermann, Richard , Schwappach, Blanche , Weissman, Jonathan S. , Schuldiner, Maya
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 0028-0836 ; DOI: 10.1038/nature20169
Zum Text:
SendSend as email Add to Book BagAdd to Book Bag
Staff View
recordid: gale_ofa472287111
title: The SND proteins constitute an alternative targeting route to the endoplasmic reticulum.(Report)
format: Article
creator:
  • Aviram, Naama
  • Ast, Tslil
  • Costa, Elizabeth A.
  • Arakel, Eric C.
  • Chuartzman, Silvia G.
  • Jan, Calvin H.
  • Hadenteufel, Sarah
  • Dudek, Johanna
  • Jung, Martin
  • Schorr, Stefan
  • Zimmermann, Richard
  • Schwappach, Blanche
  • Weissman, Jonathan S.
  • Schuldiner, Maya
subjects:
  • Cellular Proteins – Physiological Aspects
  • Endoplasmic Reticulum – Physiological Aspects
ispartof: Nature, Dec 1, 2016, Vol.540(7631), p.134(5)
description: In eukaryotes, up to one-third of cellular proteins are targeted to the endoplasmic reticulum, where they undergo folding, processing, sorting and trafficking to subsequent endomembrane compartments. Targeting to the endoplasmic reticulum has been shown to occur co-translationally by the signal recognition particle (SRP) pathway or post-translationally by the mammalian transmembrane recognition complex of 40 kDa (TRC40) and homologous yeast guided entry of tail-anchored proteins (GET) pathways. Despite the range of proteins that can be catered for by these two pathways, many proteins are still known to be independent of both SRP and GET, so there seems to be a critical need for an additional dedicated pathway for endoplasmic reticulum relay. We set out to uncover additional targeting proteins using unbiased high-content screening approaches. To this end, we performed a systematic visual screen using the yeast Saccharomyces cerevisiae, and uncovered three uncharacterized proteins whose loss affected targeting. We suggest that these proteins work together and demonstrate that they function in parallel with SRP and GET to target a broad range of substrates to the endoplasmic reticulum. The three proteins, which we name Snd1, Snd2 and Snd3 (for SRP-independent targeting), can synthetically compensate for the loss of both the SRP and GET pathways, and act as a backup targeting system. This explains why it has previously been difficult to demonstrate complete loss of targeting for some substrates. Our discovery thus puts in place an essential piece of the endoplasmic reticulum targeting puzzle, highlighting how the targeting apparatus of the eukaryotic cell is robust, interlinked and flexible.
language: eng
source:
identifier: ISSN: 0028-0836 ; DOI: 10.1038/nature20169
fulltext: fulltext
issn:
  • 0028-0836
  • 00280836
url: Link


@attributes
ID589703671
RANK0.07
NO1
SEARCH_ENGINEprimo_central_multiple_fe
SEARCH_ENGINE_TYPEPrimo Central Search Engine
LOCALfalse
PrimoNMBib
record
control
sourcerecordid472287111
sourceidgale_ofa
recordidTN_gale_ofa472287111
sourceformatXML
sourcesystemPC
pqid1845252862
galeid472287111
display
typearticle
titleThe SND proteins constitute an alternative targeting route to the endoplasmic reticulum.(Report)
creatorAviram, Naama ; Ast, Tslil ; Costa, Elizabeth A. ; Arakel, Eric C. ; Chuartzman, Silvia G. ; Jan, Calvin H. ; Hadenteufel, Sarah ; Dudek, Johanna ; Jung, Martin ; Schorr, Stefan ; Zimmermann, Richard ; Schwappach, Blanche ; Weissman, Jonathan S. ; Schuldiner, Maya
ispartofNature, Dec 1, 2016, Vol.540(7631), p.134(5)
identifierISSN: 0028-0836 ; DOI: 10.1038/nature20169
subjectCellular Proteins – Physiological Aspects ; Endoplasmic Reticulum – Physiological Aspects
languageeng
source
descriptionIn eukaryotes, up to one-third of cellular proteins are targeted to the endoplasmic reticulum, where they undergo folding, processing, sorting and trafficking to subsequent endomembrane compartments. Targeting to the endoplasmic reticulum has been shown to occur co-translationally by the signal recognition particle (SRP) pathway or post-translationally by the mammalian transmembrane recognition complex of 40 kDa (TRC40) and homologous yeast guided entry of tail-anchored proteins (GET) pathways. Despite the range of proteins that can be catered for by these two pathways, many proteins are still known to be independent of both SRP and GET, so there seems to be a critical need for an additional dedicated pathway for endoplasmic reticulum relay. We set out to uncover additional targeting proteins using unbiased high-content screening approaches. To this end, we performed a systematic visual screen using the yeast Saccharomyces cerevisiae, and uncovered three uncharacterized proteins whose loss affected targeting. We suggest that these proteins work together and demonstrate that they function in parallel with SRP and GET to target a broad range of substrates to the endoplasmic reticulum. The three proteins, which we name Snd1, Snd2 and Snd3 (for SRP-independent targeting), can synthetically compensate for the loss of both the SRP and GET pathways, and act as a backup targeting system. This explains why it has previously been difficult to demonstrate complete loss of targeting for some substrates. Our discovery thus puts in place an essential piece of the endoplasmic reticulum targeting puzzle, highlighting how the targeting apparatus of the eukaryotic cell is robust, interlinked and flexible.
version7
lds50peer_reviewed
links
openurl$$Topenurl_article
openurlfulltext$$Topenurlfull_article
search
scope
0gale_onefilea
1gale_onefileg
2OneFile
creatorcontrib
0Aviram, Naama
1Ast, Tslil
2Costa, Elizabeth A
3Arakel, Eric C
4Chuartzman, Silvia G
5Jan, Calvin H
6Hadenteufel, Sarah
7Dudek, Johanna
8Jung, Martin
9Schorr, Stefan
10Zimmermann, Richard
11Schwappach, Blanche
12Weissman, Jonathan S
13Schuldiner, Maya
titleThe SND proteins constitute an alternative targeting route to the endoplasmic reticulum.(Report)
subject
0Cellular proteins–Physiological aspects
1Endoplasmic reticulum–Physiological aspects
general
010.1038/nature20169
1English
2Nature Publishing Group
3Cengage Learning, Inc.
sourceidgale_ofa
recordidgale_ofa472287111
issn
00028-0836
100280836
rsrctypearticle
creationdate2016
startdate20161201
enddate20161201
recordtypearticle
addtitleNature
searchscopeOneFile
citationpf 134 vol 540 issue 7631
lsr30VSR-Enriched:[galeid, eissn, description, pqid, pages]
sort
titleThe SND proteins constitute an alternative targeting route to the endoplasmic reticulum.(Report)
authorAviram, Naama ; Ast, Tslil ; Costa, Elizabeth A. ; Arakel, Eric C. ; Chuartzman, Silvia G. ; Jan, Calvin H. ; Hadenteufel, Sarah ; Dudek, Johanna ; Jung, Martin ; Schorr, Stefan ; Zimmermann, Richard ; Schwappach, Blanche ; Weissman, Jonathan S. ; Schuldiner, Maya
creationdate20161201
lso0120161201
facets
frbrgroupid8837883305176313546
frbrtype5
newrecords20161206
languageeng
creationdate2016
topic
0Cellular Proteins–Physiological Aspects
1Endoplasmic Reticulum–Physiological Aspects
collectionOneFile (GALE)
prefilterarticles
rsrctypearticles
creatorcontrib
0Aviram, Naama
1Ast, Tslil
2Costa, Elizabeth A.
3Arakel, Eric C.
4Chuartzman, Silvia G.
5Jan, Calvin H.
6Hadenteufel, Sarah
7Dudek, Johanna
8Jung, Martin
9Schorr, Stefan
10Zimmermann, Richard
11Schwappach, Blanche
12Weissman, Jonathan S.
13Schuldiner, Maya
jtitleNature
toplevelpeer_reviewed
delivery
delcategoryRemote Search Resource
fulltextfulltext
addata
aulast
0Aviram
1Ast
2Costa
3Arakel
4Chuartzman
5Jan
6Hadenteufel
7Dudek
8Jung
9Schorr
10Zimmermann
11Schwappach
12Weissman
13Schuldiner
aufirst
0Naama
1Tslil
2Elizabeth A.
3Eric C.
4Silvia G.
5Calvin H.
6Sarah
7Johanna
8Martin
9Stefan
10Richard
11Blanche
12Jonathan S.
13Maya
au
0Aviram, Naama
1Ast, Tslil
2Costa, Elizabeth A.
3Arakel, Eric C.
4Chuartzman, Silvia G.
5Jan, Calvin H.
6Hadenteufel, Sarah
7Dudek, Johanna
8Jung, Martin
9Schorr, Stefan
10Zimmermann, Richard
11Schwappach, Blanche
12Weissman, Jonathan S.
13Schuldiner, Maya
atitleThe SND proteins constitute an alternative targeting route to the endoplasmic reticulum.
jtitleNature
risdate20161201
volume540
issue7631
spage134
issn0028-0836
formatjournal
genrearticle
ristypeJOUR
pubNature Publishing Group
doi10.1038/nature20169
lad01gale_ofa
pages134-138
eissn14764687
date2016-12-01