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Electrochemical Monitoring of Saos-2 Cell Differentiation on Pyrolytic Carbon Electrodes

Byline: Yasmin Mohamed Hassan, Leonardo Massa, Claudia Caviglia, Stephan Sylvest Keller Keywords: Osteoblast; Alkaline phosphatase; Electrochemical impedance spectroscopy; Square wave voltammetry; p-aminophenol Abstract In this study we establish an electrochemical platform based on two dimensional... Full description

Journal Title: Electroanalysis 2019, Vol.31(2), p.256(11)
Main Author: Mohamed Hassan, Yasmin
Other Authors: Massa, Leonardo , Caviglia, Claudia , Sylvest Keller, Stephan
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 1040-0397 ; DOI: 10.1002/elan.201800429
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recordid: gale_ofa573117583
title: Electrochemical Monitoring of Saos-2 Cell Differentiation on Pyrolytic Carbon Electrodes
format: Article
creator:
  • Mohamed Hassan, Yasmin
  • Massa, Leonardo
  • Caviglia, Claudia
  • Sylvest Keller, Stephan
subjects:
  • Electrodes – Analysis
  • Cell Differentiation – Analysis
  • Phosphatases – Analysis
ispartof: Electroanalysis, 2019, Vol.31(2), p.256(11)
description: Byline: Yasmin Mohamed Hassan, Leonardo Massa, Claudia Caviglia, Stephan Sylvest Keller Keywords: Osteoblast; Alkaline phosphatase; Electrochemical impedance spectroscopy; Square wave voltammetry; p-aminophenol Abstract In this study we establish an electrochemical platform based on two dimensional (2D) pyrolytic carbon electrodes for in vitro analysis of osteoblast differentiation. Electrochemical impedance spectroscopy (EIS) was used to monitor cell adhesion and proliferation, while an electrochemical assay based on square wave voltammetry (SWV) was applied to measure the activity of the differentiation marker alkaline phosphatase (ALP). 2D pyrolytic carbon electrodes were fabricated and used to monitor Saos-2 cell differentiation for a period of up to 21 days. With this method it was possible to detect a faster increase of ALP activity for cells cultured in medium supplemented with differentiation factors compared to cells cultured in growth medium. This was confirmed by the results obtained with Alizarin Red staining, showing that cells subjected to osteogenic medium went through the entire differentiation process, from proliferation to mineralization. Finally, for the first time, real-time monitoring of ALP activity combined with continuous EIS monitoring of the same cell culture was achieved using the pyrolytic carbon electrodes. Supporting information: Additional Supporting Information may be found in the online version of this article As a service to our authors and readers, this journal provides supporting information supplied by the authors. Such materials are peer reviewed and may be re-organized for online delivery, but are not copy-edited or typeset. Technical support issues arising from supporting information (other than missing files) should be addressed to the authors. CAPTION(S): Supplementary
language: eng
source:
identifier: ISSN: 1040-0397 ; DOI: 10.1002/elan.201800429
fulltext: fulltext
issn:
  • 1040-0397
  • 10400397
url: Link


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titleElectrochemical Monitoring of Saos-2 Cell Differentiation on Pyrolytic Carbon Electrodes
creatorMohamed Hassan, Yasmin ; Massa, Leonardo ; Caviglia, Claudia ; Sylvest Keller, Stephan
ispartofElectroanalysis, 2019, Vol.31(2), p.256(11)
identifierISSN: 1040-0397 ; DOI: 10.1002/elan.201800429
subjectElectrodes – Analysis ; Cell Differentiation – Analysis ; Phosphatases – Analysis
descriptionByline: Yasmin Mohamed Hassan, Leonardo Massa, Claudia Caviglia, Stephan Sylvest Keller Keywords: Osteoblast; Alkaline phosphatase; Electrochemical impedance spectroscopy; Square wave voltammetry; p-aminophenol Abstract In this study we establish an electrochemical platform based on two dimensional (2D) pyrolytic carbon electrodes for in vitro analysis of osteoblast differentiation. Electrochemical impedance spectroscopy (EIS) was used to monitor cell adhesion and proliferation, while an electrochemical assay based on square wave voltammetry (SWV) was applied to measure the activity of the differentiation marker alkaline phosphatase (ALP). 2D pyrolytic carbon electrodes were fabricated and used to monitor Saos-2 cell differentiation for a period of up to 21 days. With this method it was possible to detect a faster increase of ALP activity for cells cultured in medium supplemented with differentiation factors compared to cells cultured in growth medium. This was confirmed by the results obtained with Alizarin Red staining, showing that cells subjected to osteogenic medium went through the entire differentiation process, from proliferation to mineralization. Finally, for the first time, real-time monitoring of ALP activity combined with continuous EIS monitoring of the same cell culture was achieved using the pyrolytic carbon electrodes. Supporting information: Additional Supporting Information may be found in the online version of this article As a service to our authors and readers, this journal provides supporting information supplied by the authors. Such materials are peer reviewed and may be re-organized for online delivery, but are not copy-edited or typeset. Technical support issues arising from supporting information (other than missing files) should be addressed to the authors. CAPTION(S): Supplementary
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abstractByline: Yasmin Mohamed Hassan, Leonardo Massa, Claudia Caviglia, Stephan Sylvest Keller Keywords: Osteoblast; Alkaline phosphatase; Electrochemical impedance spectroscopy; Square wave voltammetry; p-aminophenol Abstract In this study we establish an electrochemical platform based on two dimensional (2D) pyrolytic carbon electrodes for in vitro analysis of osteoblast differentiation. Electrochemical impedance spectroscopy (EIS) was used to monitor cell adhesion and proliferation, while an electrochemical assay based on square wave voltammetry (SWV) was applied to measure the activity of the differentiation marker alkaline phosphatase (ALP). 2D pyrolytic carbon electrodes were fabricated and used to monitor Saos-2 cell differentiation for a period of up to 21 days. With this method it was possible to detect a faster increase of ALP activity for cells cultured in medium supplemented with differentiation factors compared to cells cultured in growth medium. This was confirmed by the results obtained with Alizarin Red staining, showing that cells subjected to osteogenic medium went through the entire differentiation process, from proliferation to mineralization. Finally, for the first time, real-time monitoring of ALP activity combined with continuous EIS monitoring of the same cell culture was achieved using the pyrolytic carbon electrodes. Supporting information: Additional Supporting Information may be found in the online version of this article As a service to our authors and readers, this journal provides supporting information supplied by the authors. Such materials are peer reviewed and may be re-organized for online delivery, but are not copy-edited or typeset. Technical support issues arising from supporting information (other than missing files) should be addressed to the authors. CAPTION(S): Supplementary
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