schliessen

Filtern

 

Bibliotheken

Synthesis of fluorescent gold nanodot-liposome hybrids for detection of phospholipase C and its inhibitor.(Report)(Author abstract)

We report the synthesis of fluorescent 11-mercaptoundecanoic acid-gold nanodot-liposome (11-MUA-Au ND/Lip) hybrids by incorporation of gold nanoparticles (3 nm) and 11-MUA molecules in hydrophobic phospholipid membranes that self-assemble to form small unilamellar vesicles. A simple and homogeneous... Full description

Journal Title: Analytical Chemistry Sept 17, 2013, Vol.85(18), p.8834
Main Author: Wei-Yu Chen
Other Authors: Li-Yi Chen , Chung-Mao Ou , Chih-Ching Huang , Shih-Chung Wei , Huan-Tsung Chang
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 0003-2700
Zum Text:
SendSend as email Add to Book BagAdd to Book Bag
Staff View
recordid: gale_ofg362778406
title: Synthesis of fluorescent gold nanodot-liposome hybrids for detection of phospholipase C and its inhibitor.(Report)(Author abstract)
format: Article
creator:
  • Wei-Yu Chen
  • Li-Yi Chen
  • Chung-Mao Ou
  • Chih-Ching Huang
  • Shih-Chung Wei
  • Huan-Tsung Chang
subjects:
  • Phospholipases – Methods
  • Nanoparticles – Research
  • Liposomes – Usage
  • Fluorescence – Research
ispartof: Analytical Chemistry, Sept 17, 2013, Vol.85(18), p.8834
description: We report the synthesis of fluorescent 11-mercaptoundecanoic acid-gold nanodot-liposome (11-MUA-Au ND/Lip) hybrids by incorporation of gold nanoparticles (3 nm) and 11-MUA molecules in hydrophobic phospholipid membranes that self-assemble to form small unilamellar vesicles. A simple and homogeneous fluorescence assay for phospholipase C (PLC) was developed on the basis of the fluorescence quenching of 11-MUA-Au ND/Lip hybrids in aqueous solution. The fluorescence of the 11-MUA-Au ND/Lip hybrids is quenched by oxygen (O2) molecules in solution, and quenching is reduced in the presence of PLC. PLC catalyzes the hydrolysis of phosphatidylcholine units from Lip to yield diacylglycerol (DAG) and phosphocholine (PC) products, leading to the decomposition of Lip. The diacylglycerol further interacts with 11-MUA-Au NDs via hydrophobic interactions, leading to inhibition of O2 quenching. The 11-MUA-Au ND/Lip probe provides a limit of detection (at a signal-to-noise ratio of 3) of 0.21 nM for PLC, with high selectivity over other proteins, enzymes, and phospholipases. We have validated the practicality of using this probe for the determination of PLC concentrations in breast cancer cells (MCF-7 and MDA-MB-231 cell lines) and nontumor cells (MCF-10A cell line), revealing that the PLC activity in the first two is at least 1.5-fold higher than that in the third. An inhibitor assay using 11-MUA-Au ND/Lip hybrids demonstrated that tricyclodecan-9-yl potassium xanthate (D609) inhibits PLC (10 nM) with an IC50 value of 3.81 [+ or -] 0.22 ?M. This simple, sensitive, and selective approach holds great potential for detection of PLC in cancer cells and for the screening of anti-PLC drugs.
language: eng
source:
identifier: ISSN: 0003-2700
fulltext: no_fulltext
issn:
  • 0003-2700
  • 00032700
url: Link


@attributes
ID910892202
RANK0.06999999
NO1
SEARCH_ENGINEprimo_central_multiple_fe
SEARCH_ENGINE_TYPEPrimo Central Search Engine
LOCALfalse
PrimoNMBib
record
control
sourcerecordid362778406
sourceidgale_ofg
recordidTN_gale_ofg362778406
sourceformatXML
sourcesystemOther
pqid1433513801
galeid362778406
display
typearticle
titleSynthesis of fluorescent gold nanodot-liposome hybrids for detection of phospholipase C and its inhibitor.(Report)(Author abstract)
creatorWei-Yu Chen ; Li-Yi Chen ; Chung-Mao Ou ; Chih-Ching Huang ; Shih-Chung Wei ; Huan-Tsung Chang
ispartofAnalytical Chemistry, Sept 17, 2013, Vol.85(18), p.8834
identifierISSN: 0003-2700
subjectPhospholipases – Methods ; Nanoparticles – Research ; Liposomes – Usage ; Fluorescence – Research
descriptionWe report the synthesis of fluorescent 11-mercaptoundecanoic acid-gold nanodot-liposome (11-MUA-Au ND/Lip) hybrids by incorporation of gold nanoparticles (3 nm) and 11-MUA molecules in hydrophobic phospholipid membranes that self-assemble to form small unilamellar vesicles. A simple and homogeneous fluorescence assay for phospholipase C (PLC) was developed on the basis of the fluorescence quenching of 11-MUA-Au ND/Lip hybrids in aqueous solution. The fluorescence of the 11-MUA-Au ND/Lip hybrids is quenched by oxygen (O2) molecules in solution, and quenching is reduced in the presence of PLC. PLC catalyzes the hydrolysis of phosphatidylcholine units from Lip to yield diacylglycerol (DAG) and phosphocholine (PC) products, leading to the decomposition of Lip. The diacylglycerol further interacts with 11-MUA-Au NDs via hydrophobic interactions, leading to inhibition of O2 quenching. The 11-MUA-Au ND/Lip probe provides a limit of detection (at a signal-to-noise ratio of 3) of 0.21 nM for PLC, with high selectivity over other proteins, enzymes, and phospholipases. We have validated the practicality of using this probe for the determination of PLC concentrations in breast cancer cells (MCF-7 and MDA-MB-231 cell lines) and nontumor cells (MCF-10A cell line), revealing that the PLC activity in the first two is at least 1.5-fold higher than that in the third. An inhibitor assay using 11-MUA-Au ND/Lip hybrids demonstrated that tricyclodecan-9-yl potassium xanthate (D609) inhibits PLC (10 nM) with an IC50 value of 3.81 [+ or -] 0.22 ?M. This simple, sensitive, and selective approach holds great potential for detection of PLC in cancer cells and for the screening of anti-PLC drugs.
languageeng
source
version6
lds50peer_reviewed
links
openurl$$Topenurl_article
openurlfulltext$$Topenurlfull_article
search
scope
0gale_onefileg
1gale_onefileg
2OneFile
creatorcontrib
0Wei-Yu Chen
1Li-Yi Chen
2Chung-Mao Ou
3Chih-Ching Huang
4Shih-Chung Wei
5Huan-Tsung Chang
titleSynthesis of fluorescent gold nanodot-liposome hybrids for detection of phospholipase C and its inhibitor.(Report)(Author abstract)
descriptionWe report the synthesis of fluorescent 11-mercaptoundecanoic acid-gold nanodot-liposome (11-MUA-Au ND/Lip) hybrids by incorporation of gold nanoparticles (3 nm) and 11-MUA molecules in hydrophobic phospholipid membranes that self-assemble to form small unilamellar vesicles. A simple and homogeneous fluorescence assay for phospholipase C (PLC) was developed on the basis of the fluorescence quenching of 11-MUA-Au ND/Lip hybrids in aqueous solution. The fluorescence of the 11-MUA-Au ND/Lip hybrids is quenched by oxygen (O2) molecules in solution, and quenching is reduced in the presence of PLC. PLC catalyzes the hydrolysis of phosphatidylcholine units from Lip to yield diacylglycerol (DAG) and phosphocholine (PC) products, leading to the decomposition of Lip. The diacylglycerol further interacts with 11-MUA-Au NDs via hydrophobic interactions, leading to inhibition of O2 quenching. The 11-MUA-Au ND/Lip probe provides a limit of detection (at a signal-to-noise ratio of 3) of 0.21 nM for PLC, with high selectivity over other proteins, enzymes, and phospholipases. We have validated the practicality of using this probe for the determination of PLC concentrations in breast cancer cells (MCF-7 and MDA-MB-231 cell lines) and nontumor cells (MCF-10A cell line), revealing that the PLC activity in the first two is at least 1.5-fold higher than that in the third. An inhibitor assay using 11-MUA-Au ND/Lip hybrids demonstrated that tricyclodecan-9-yl potassium xanthate (D609) inhibits PLC (10 nM) with an IC50 value of 3.81 [+ or -] 0.22 ?M. This simple, sensitive, and selective approach holds great potential for detection of PLC in cancer cells and for the screening of anti-PLC drugs.
subject
0Phospholipases–Methods
1Nanoparticles–Research
2Liposomes–Usage
3Fluorescence–Research
42834
5Pharmaceutical preparations
6Biological Product (except Diagnostic) Manufacturing
7Pharmaceutical Preparation Manufacturing
8325414
9325412
general
0English
1American Chemical Society
2Cengage Learning, Inc.
sourceidgale_ofg
recordidgale_ofg362778406
issn
00003-2700
100032700
rsrctypearticle
creationdate2013
startdate20130917
enddate20130917
recordtypearticle
addtitleAnalytical Chemistry
searchscopeOneFile
citationpf 8834 vol 85 issue 18
lsr30VSR-Enriched:[pqid, doi, pages, eissn, galeid]
sort
titleSynthesis of fluorescent gold nanodot-liposome hybrids for detection of phospholipase C and its inhibitor.(Report)(Author abstract)
authorWei-Yu Chen ; Li-Yi Chen ; Chung-Mao Ou ; Chih-Ching Huang ; Shih-Chung Wei ; Huan-Tsung Chang
creationdate20130917
lso0120130917
facets
frbrgroupid6365152650874312257
frbrtype5
languageeng
creationdate2013
topic
0Phospholipases–Methods
1Nanoparticles–Research
2Liposomes–Usage
3Fluorescence–Research
collectionOneFile (GALE)
prefilterarticles
rsrctypearticles
creatorcontrib
0Wei-Yu Chen
1Li-Yi Chen
2Chung-Mao Ou
3Chih-Ching Huang
4Shih-Chung Wei
5Huan-Tsung Chang
jtitleAnalytical Chemistry
toplevelpeer_reviewed
delivery
delcategoryRemote Search Resource
fulltextno_fulltext
addata
aulast
0Wei-Yu Chen
1Li-Yi Chen
2Chung-Mao Ou
3Chih-Ching Huang
4Shih-Chung Wei
5Huan-Tsung Chang
au
0Wei-Yu Chen
1Li-Yi Chen
2Chung-Mao Ou
3Chih-Ching Huang
4Shih-Chung Wei
5Huan-Tsung Chang
atitleSynthesis of fluorescent gold nanodot-liposome hybrids for detection of phospholipase C and its inhibitor.
jtitleAnalytical Chemistry
risdate20130917
volume85
issue18
spage8834
issn0003-2700
formatjournal
genrearticle
ristypeJOUR
abstractWe report the synthesis of fluorescent 11-mercaptoundecanoic acid-gold nanodot-liposome (11-MUA-Au ND/Lip) hybrids by incorporation of gold nanoparticles (3 nm) and 11-MUA molecules in hydrophobic phospholipid membranes that self-assemble to form small unilamellar vesicles. A simple and homogeneous fluorescence assay for phospholipase C (PLC) was developed on the basis of the fluorescence quenching of 11-MUA-Au ND/Lip hybrids in aqueous solution. The fluorescence of the 11-MUA-Au ND/Lip hybrids is quenched by oxygen (O2) molecules in solution, and quenching is reduced in the presence of PLC. PLC catalyzes the hydrolysis of phosphatidylcholine units from Lip to yield diacylglycerol (DAG) and phosphocholine (PC) products, leading to the decomposition of Lip. The diacylglycerol further interacts with 11-MUA-Au NDs via hydrophobic interactions, leading to inhibition of O2 quenching. The 11-MUA-Au ND/Lip probe provides a limit of detection (at a signal-to-noise ratio of 3) of 0.21 nM for PLC, with high selectivity over other proteins, enzymes, and phospholipases. We have validated the practicality of using this probe for the determination of PLC concentrations in breast cancer cells (MCF-7 and MDA-MB-231 cell lines) and nontumor cells (MCF-10A cell line), revealing that the PLC activity in the first two is at least 1.5-fold higher than that in the third. An inhibitor assay using 11-MUA-Au ND/Lip hybrids demonstrated that tricyclodecan-9-yl potassium xanthate (D609) inhibits PLC (10 nM) with an IC50 value of 3.81 [+ or -] 0.22 ?M. This simple, sensitive, and selective approach holds great potential for detection of PLC in cancer cells and for the screening of anti-PLC drugs.
pubAmerican Chemical Society
lad01gale_ofg
doi10.1021/ac402043t
pages8834
eissn15206882
date2013-09-17