schliessen

Filtern

 

Bibliotheken

In vitro selective growth-inhibitory effect of 8-hydroxyquinoline on Clostridium perfringens versus Bifidobacteria in a medium containing chicken ileal digesta

Clostridium perfringens-induced necrotic enteritis is generally controlled by antibiotics. However, because of increasing antibiotic resistance, other antibacterial agents are required, preferably ones that do not affect the beneficial intestinal microbiota of the host. This study evaluated the in v... Full description

Journal Title: PLOS ONE 2016
Main Author: Skrivanova, Eva
Other Authors: Van Immerseel, Filip , Hovorkova, Petra , Kokoska, Ladislav
Format: Electronic Article Electronic Article
Language: English
Subjects:
Created: 2016
ID: ISSN: 1932-6203 ; DOI: 10.1371/journal.pone.0167638
Zum Text:
SendSend as email Add to Book BagAdd to Book Bag
Staff View
recordid: ghent8515160
title: In vitro selective growth-inhibitory effect of 8-hydroxyquinoline on Clostridium perfringens versus Bifidobacteria in a medium containing chicken ileal digesta
format: Article
creator:
  • Skrivanova, Eva
  • Van Immerseel, Filip
  • Hovorkova, Petra
  • Kokoska, Ladislav
subjects:
  • Veterinary Sciences
  • Biology And Life Sciences
  • Necrotic Enteritis
  • Broiler-Chickens
  • Antimicrobial Activity
  • Essential
  • Oils
  • Microbiota
  • Acid
  • 8-Quinolinol
  • Pathogenesis
  • Antibiotics
  • Clioquinol
ispartof: PLOS ONE, 2016
description: Clostridium perfringens-induced necrotic enteritis is generally controlled by antibiotics. However, because of increasing antibiotic resistance, other antibacterial agents are required, preferably ones that do not affect the beneficial intestinal microbiota of the host. This study evaluated the in vitro selective growth-inhibitory effect of 8-hydroxyquinoline (8HQ) on C. perfringens vs. bifidobacteria in a medium containing chicken ileal digesta. Prior to the experiments, the minimum inhibitory concentrations of 8HQ and penicillin G were determined by broth microdilution assay. The minimum inhibitory concentration values of 8HQ for C. perfringens were 16-32 times lower than the values for bifidobacteria. Treatment of autoclaved and non-autoclaved chicken ileal digesta with 8HQ showed a selective anticlostridial effect. After incubation of C. perfringens with autoclaved ileal digesta for 3 h, all 8HQ concentrations tested (32-2048 mu g/mL) significantly reduced C. perfringens bacterial count. In contrast, the same treatment had no or only a slight effect on bifidobacteria counts. Unlike 8HQ, penicillin G did not exhibit any selectivity. Similar results were obtained after incubation for 24 h. In non-autoclaved ileal digesta, all 8HQ concentrations tested significantly reduced C. perfringens bacterial counts after incubation for 30 min and 3 h, while no effect was observed on bifidobacteria. These results suggest that 8HQ may serve as a prospective veterinary compound for use against necrotic enteritis in poultry.
language: eng
source:
identifier: ISSN: 1932-6203 ; DOI: 10.1371/journal.pone.0167638
fulltext: fulltext_linktorsrc
issn:
  • 1932 6203
  • 1932-6203
url: Link


@attributes
ID1024399268
RANK0.07
NO1
SEARCH_ENGINEprimo_central_multiple_fe
SEARCH_ENGINE_TYPEPrimo Central Search Engine
LOCALfalse
PrimoNMBib
record
control
sourcerecordid8515160
sourceidghent
recordidTN_ghent8515160
sourcesystemOther
pqid1852659167
galeid473428080
display
typearticle
titleIn vitro selective growth-inhibitory effect of 8-hydroxyquinoline on Clostridium perfringens versus Bifidobacteria in a medium containing chicken ileal digesta
creatorSkrivanova, Eva ; Van Immerseel, Filip ; Hovorkova, Petra ; Kokoska, Ladislav
creationdate2016
ispartofPLOS ONE, 2016
identifier ISSN: 1932-6203 ; DOI: 10.1371/journal.pone.0167638
subjectVeterinary Sciences ; Biology And Life Sciences ; Necrotic Enteritis ; Broiler-Chickens ; Antimicrobial Activity ; Essential ; Oils ; Microbiota ; Acid ; 8-Quinolinol ; Pathogenesis ; Antibiotics ; Clioquinol
descriptionClostridium perfringens-induced necrotic enteritis is generally controlled by antibiotics. However, because of increasing antibiotic resistance, other antibacterial agents are required, preferably ones that do not affect the beneficial intestinal microbiota of the host. This study evaluated the in vitro selective growth-inhibitory effect of 8-hydroxyquinoline (8HQ) on C. perfringens vs. bifidobacteria in a medium containing chicken ileal digesta. Prior to the experiments, the minimum inhibitory concentrations of 8HQ and penicillin G were determined by broth microdilution assay. The minimum inhibitory concentration values of 8HQ for C. perfringens were 16-32 times lower than the values for bifidobacteria. Treatment of autoclaved and non-autoclaved chicken ileal digesta with 8HQ showed a selective anticlostridial effect. After incubation of C. perfringens with autoclaved ileal digesta for 3 h, all 8HQ concentrations tested (32-2048 mu g/mL) significantly reduced C. perfringens bacterial count. In contrast, the same treatment had no or only a slight effect on bifidobacteria counts. Unlike 8HQ, penicillin G did not exhibit any selectivity. Similar results were obtained after incubation for 24 h. In non-autoclaved ileal digesta, all 8HQ concentrations tested significantly reduced C. perfringens bacterial counts after incubation for 30 min and 3 h, while no effect was observed on bifidobacteria. These results suggest that 8HQ may serve as a prospective veterinary compound for use against necrotic enteritis in poultry.
languageeng
source
version10
oafree_for_read
lds50peer_reviewed
links
openurl$$Topenurl_article
linktorsrc
0$$Uhttps://biblio.ugent.be/publication/8515160$$EView_full_text_in_Ghent_University
1$$Uhttps://biblio.ugent.be/publication/8515160/file/8515161$$EView_full_text_in_Ghent_University
openurlfulltext$$Topenurlfull_article
search
creatorcontrib
0Skrivanova, Eva
1Van Immerseel, Filip
2Hovorkova, Petra
3Kokoska, Ladislav
titleIn vitro selective growth-inhibitory effect of 8-hydroxyquinoline on Clostridium perfringens versus Bifidobacteria in a medium containing chicken ileal digesta
descriptionClostridium perfringens-induced necrotic enteritis is generally controlled by antibiotics. However, because of increasing antibiotic resistance, other antibacterial agents are required, preferably ones that do not affect the beneficial intestinal microbiota of the host. This study evaluated the in vitro selective growth-inhibitory effect of 8-hydroxyquinoline (8HQ) on C. perfringens vs. bifidobacteria in a medium containing chicken ileal digesta. Prior to the experiments, the minimum inhibitory concentrations of 8HQ and penicillin G were determined by broth microdilution assay. The minimum inhibitory concentration values of 8HQ for C. perfringens were 16-32 times lower than the values for bifidobacteria. Treatment of autoclaved and non-autoclaved chicken ileal digesta with 8HQ showed a selective anticlostridial effect. After incubation of C. perfringens with autoclaved ileal digesta for 3 h, all 8HQ concentrations tested (32-2048 mu g/mL) significantly reduced C. perfringens bacterial count. In contrast, the same treatment had no or only a slight effect on bifidobacteria counts. Unlike 8HQ, penicillin G did not exhibit any selectivity. Similar results were obtained after incubation for 24 h. In non-autoclaved ileal digesta, all 8HQ concentrations tested significantly reduced C. perfringens bacterial counts after incubation for 30 min and 3 h, while no effect was observed on bifidobacteria. These results suggest that 8HQ may serve as a prospective veterinary compound for use against necrotic enteritis in poultry.
subject
0Veterinary Sciences
1Biology And Life Sciences
2Necrotic Enteritis
3Broiler-Chickens
4Antimicrobial Activity
5Essential
6Oils
7Microbiota
8Acid
98-Quinolinol
10Pathogenesis
11Antibiotics
12Clioquinol
general
0English
110.1371/journal.pone.0167638
2Ghent University Academic Bibliography
sourceidghent
recordidghent8515160
issn
01932 6203
11932-6203
rsrctypearticle
creationdate2016
addtitlePLOS ONE
searchscopeghent
scopeghent
lsr30VSR-Enriched:[pqid, galeid, pages, vol, issue]
sort
titleIn vitro selective growth-inhibitory effect of 8-hydroxyquinoline on Clostridium perfringens versus Bifidobacteria in a medium containing chicken ileal digesta
authorSkrivanova, Eva ; Van Immerseel, Filip ; Hovorkova, Petra ; Kokoska, Ladislav
creationdate20160000
facets
frbrgroupid8437217318499623671
frbrtype5
newrecords20190515
languageeng
creationdate2016
topic
0Veterinary Sciences
1Biology And Life Sciences
2Necrotic Enteritis
3Broiler-Chickens
4Antimicrobial Activity
5Essential
6Oils
7Microbiota
8Acid
98-Quinolinol
10Pathogenesis
11Antibiotics
12Clioquinol
collectionGhent University Academic Bibliography
prefilterarticles
rsrctypearticles
creatorcontrib
0Skrivanova, Eva
1Van Immerseel, Filip
2Hovorkova, Petra
3Kokoska, Ladislav
jtitlePLOS ONE
toplevelpeer_reviewed
delivery
delcategoryRemote Search Resource
fulltextfulltext_linktorsrc
addata
aulast
0Skrivanova
1Van Immerseel
2Hovorkova
3Kokoska
aufirst
0Eva
1Filip
2Petra
3Ladislav
au
0Skrivanova, Eva
1Van Immerseel, Filip
2Hovorkova, Petra
3Kokoska, Ladislav
atitleIn vitro selective growth-inhibitory effect of 8-hydroxyquinoline on Clostridium perfringens versus Bifidobacteria in a medium containing chicken ileal digesta
jtitlePLOS ONE
date2016
risdate2016
issn1932-6203
genrearticle
ristypeJOUR
abstractClostridium perfringens-induced necrotic enteritis is generally controlled by antibiotics. However, because of increasing antibiotic resistance, other antibacterial agents are required, preferably ones that do not affect the beneficial intestinal microbiota of the host. This study evaluated the in vitro selective growth-inhibitory effect of 8-hydroxyquinoline (8HQ) on C. perfringens vs. bifidobacteria in a medium containing chicken ileal digesta. Prior to the experiments, the minimum inhibitory concentrations of 8HQ and penicillin G were determined by broth microdilution assay. The minimum inhibitory concentration values of 8HQ for C. perfringens were 16-32 times lower than the values for bifidobacteria. Treatment of autoclaved and non-autoclaved chicken ileal digesta with 8HQ showed a selective anticlostridial effect. After incubation of C. perfringens with autoclaved ileal digesta for 3 h, all 8HQ concentrations tested (32-2048 mu g/mL) significantly reduced C. perfringens bacterial count. In contrast, the same treatment had no or only a slight effect on bifidobacteria counts. Unlike 8HQ, penicillin G did not exhibit any selectivity. Similar results were obtained after incubation for 24 h. In non-autoclaved ileal digesta, all 8HQ concentrations tested significantly reduced C. perfringens bacterial counts after incubation for 30 min and 3 h, while no effect was observed on bifidobacteria. These results suggest that 8HQ may serve as a prospective veterinary compound for use against necrotic enteritis in poultry.
doi10.1371/journal.pone.0167638
url
0https://biblio.ugent.be/publication/8515160
1https://biblio.ugent.be/publication/8515160/file/8515161
formatarticle
pagese0167638
volume11
issue12
oafree_for_read