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Conserved B-cell epitopes among human bocavirus species indicate potential diagnostic targets

BACKGROUND: Human bocavirus species 1-4 (HBoV1-4) have been associated with respiratory and enteric infections in children. However, the immunological mechanisms in response to HBoV infections are not fully understood. Though previous studies have shown cross-reactivities between HBoV species, the e... Full description

Journal Title: PLoS ONE 2014, Vol.9(1), p.e86960
Main Author: Zhou, Zhuo
Other Authors: Gao, Xin , Wang, Yaying , Zhou, Hongli , Wu, Chao , Paranhos-Baccalà, Gláucia , Vernet, Guy , Guo, Li , Wang, Jianwei
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 1932-6203 ; E-ISSN: 1932-6203 ; DOI: 10.1371/journal.pone.0086960
Link: https://hal.archives-ouvertes.fr/hal-00972352
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recordid: hal_soai_HAL_hal_00972352v1
title: Conserved B-cell epitopes among human bocavirus species indicate potential diagnostic targets
format: Article
creator:
  • Zhou, Zhuo
  • Gao, Xin
  • Wang, Yaying
  • Zhou, Hongli
  • Wu, Chao
  • Paranhos-Baccalà, Gláucia
  • Vernet, Guy
  • Guo, Li
  • Wang, Jianwei
subjects:
  • Life Sciences
  • Sciences (General)
ispartof: PLoS ONE, 2014, Vol.9(1), p.e86960
description: BACKGROUND: Human bocavirus species 1-4 (HBoV1-4) have been associated with respiratory and enteric infections in children. However, the immunological mechanisms in response to HBoV infections are not fully understood. Though previous studies have shown cross-reactivities between HBoV species, the epitopes responsible for this phenomenon remain unknown. In this study, we used genomic and immunologic approaches to identify the reactive epitopes conserved across multiple HBoV species and explored their potential as the basis of a novel diagnostic test for HBoVs. METHODOLOGY/PRINCIPAL FINDINGS: We generated HBoV1-3 VP2 gene fragment phage display libraries (GFPDLs) and used these libraries to analyze mouse antisera against VP2 protein of HBoV1, 2, and 3, and human sera positive for HBoVs. Using this approach, we mapped four epitope clusters of HBoVs and identified two immunodominant peptides--P1 (¹MSDTDIQDQQPDTVDAPQNT²⁰), and P2 (¹⁶²EHAYPNASHPWDEDVMPDL¹⁸⁰)--that are conserved...
language: eng
source:
identifier: ISSN: 1932-6203 ; E-ISSN: 1932-6203 ; DOI: 10.1371/journal.pone.0086960
fulltext: fulltext
issn:
  • 1932-6203
  • 19326203
url: Link


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titleConserved B-cell epitopes among human bocavirus species indicate potential diagnostic targets
creatorZhou, Zhuo ; Gao, Xin ; Wang, Yaying ; Zhou, Hongli ; Wu, Chao ; Paranhos-Baccalà, Gláucia ; Vernet, Guy ; Guo, Li ; Wang, Jianwei
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descriptionBACKGROUND: Human bocavirus species 1-4 (HBoV1-4) have been associated with respiratory and enteric infections in children. However, the immunological mechanisms in response to HBoV infections are not fully understood. Though previous studies have shown cross-reactivities between HBoV species, the epitopes responsible for this phenomenon remain unknown. In this study, we used genomic and immunologic approaches to identify the reactive epitopes conserved across multiple HBoV species and explored their potential as the basis of a novel diagnostic test for HBoVs. METHODOLOGY/PRINCIPAL FINDINGS: We generated HBoV1-3 VP2 gene fragment phage display libraries (GFPDLs) and used these libraries to analyze mouse antisera against VP2 protein of HBoV1, 2, and 3, and human sera positive for HBoVs. Using this approach, we mapped four epitope clusters of HBoVs and identified two immunodominant peptides--P1 (¹MSDTDIQDQQPDTVDAPQNT²⁰), and P2 (¹⁶²EHAYPNASHPWDEDVMPDL¹⁸⁰)--that are conserved...
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titleConserved B-cell epitopes among human bocavirus species indicate potential diagnostic targets
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BACKGROUND: Human bocavirus species 1-4 (HBoV1-4) have been associated with respiratory and enteric infections in children. However, the immunological mechanisms in response to HBoV infections are not fully understood. Though previous studies have shown cross-reactivities between HBoV species, the epitopes responsible for this phenomenon remain unknown. In this study, we used genomic and immunologic approaches to identify the reactive epitopes conserved across multiple HBoV species and explored their potential as the basis of a novel diagnostic test for HBoVs. METHODOLOGY/PRINCIPAL FINDINGS: We generated HBoV1-3 VP2 gene fragment phage display libraries (GFPDLs) and used these libraries to analyze mouse antisera against VP2 protein of HBoV1, 2, and 3, and human sera positive for HBoVs. Using this approach, we mapped four epitope clusters of HBoVs and identified two immunodominant peptides--P1 (¹MSDTDIQDQQPDTVDAPQNT²⁰), and P2 (¹⁶²EHAYPNASHPWDEDVMPDL¹⁸⁰)--that are conserved...

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BACKGROUND: Human bocavirus species 1-4 (HBoV1-4) have been associated with respiratory and enteric infections in children. However, the immunological mechanisms in response to HBoV infections are not fully understood. Though previous studies have shown cross-reactivities between HBoV species, the epitopes responsible for this phenomenon remain unknown. In this study, we used genomic and immunologic approaches to identify the reactive epitopes conserved across multiple HBoV species and explored their potential as the basis of a novel diagnostic test for HBoVs. METHODOLOGY/PRINCIPAL FINDINGS: We generated HBoV1-3 VP2 gene fragment phage display libraries (GFPDLs) and used these libraries to analyze mouse antisera against VP2 protein of HBoV1, 2, and 3, and human sera positive for HBoVs. Using this approach, we mapped four epitope clusters of HBoVs and identified two immunodominant peptides--P1 (¹MSDTDIQDQQPDTVDAPQNT²⁰), and P2 (¹⁶²EHAYPNASHPWDEDVMPDL¹⁸⁰)--that are conserved...

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