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Efficient and Fast Differentiation of Human Neural Stem Cells from Human Embryonic Stem Cells for Cell Therapy

Stem cell-based therapies have been used for repairing damaged brain tissue and helping functional recovery after brain injury. Aberrance neurogenesis is related with brain injury, and multipotential neural stem cells from human embryonic stem (hES) cells provide a great promise for cell replacement... Full description

Journal Title: Stem Cells International 2017, Vol.2017, 11 pages
Main Author: Han, Xinxin
Other Authors: Yu, Liming , Ren, Jie , Wang, Min , Liu, Zhongliang , Hu, Xinyu , Hu, Daiyu , Chen, Yihong , Chen, Li , Zhang, Ying , Liu, Yuehua , Zhang, Xiaoqing , He, Hua , Gao, Zhengliang
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ID: ISSN: 1687-966X ; E-ISSN: 1687-9678 ; DOI: 10.1155/2017/9405204
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recordid: hindawi10.1155/2017/9405204
title: Efficient and Fast Differentiation of Human Neural Stem Cells from Human Embryonic Stem Cells for Cell Therapy
format: Article
creator:
  • Han, Xinxin
  • Yu, Liming
  • Ren, Jie
  • Wang, Min
  • Liu, Zhongliang
  • Hu, Xinyu
  • Hu, Daiyu
  • Chen, Yihong
  • Chen, Li
  • Zhang, Ying
  • Liu, Yuehua
  • Zhang, Xiaoqing
  • He, Hua
  • Gao, Zhengliang
subjects:
  • Stem Cell Research
  • Brain Injuries
  • Brain Damage
  • Stem Cell Transplantation
  • Cell Differentiation
  • Embryonic Stem Cells
ispartof: Stem Cells International, 2017, Vol.2017, 11 pages
description: Stem cell-based therapies have been used for repairing damaged brain tissue and helping functional recovery after brain injury. Aberrance neurogenesis is related with brain injury, and multipotential neural stem cells from human embryonic stem (hES) cells provide a great promise for cell replacement therapies. Optimized protocols for neural differentiation are necessary to produce functional human neural stem cells (hNSCs) for cell therapy. However, the qualified procedure is scarce and detailed features of hNSCs originated from hES cells are still unclear. In this study, we developed a method to obtain hNSCs from hES cells, by which we could harvest abundant hNSCs in a relatively short time. Then, we examined the expression of pluripotent and multipotent marker genes through immunostaining and confirmed differentiation potential of the differentiated hNSCs. Furthermore, we analyzed the mitotic activity of these hNSCs. In this report, we provided comprehensive features of hNSCs and delivered the knowledge about how to obtain more high-quality hNSCs from hES cells which may help to accelerate the NSC-based therapies in brain injury treatment.
language:
source:
identifier: ISSN: 1687-966X ; E-ISSN: 1687-9678 ; DOI: 10.1155/2017/9405204
fulltext: fulltext
issn:
  • 1687-966X
  • 1687966X
  • 1687-9678
  • 16879678
url: Link


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titleEfficient and Fast Differentiation of Human Neural Stem Cells from Human Embryonic Stem Cells for Cell Therapy
creatorHan, Xinxin ; Yu, Liming ; Ren, Jie ; Wang, Min ; Liu, Zhongliang ; Hu, Xinyu ; Hu, Daiyu ; Chen, Yihong ; Chen, Li ; Zhang, Ying ; Liu, Yuehua ; Zhang, Xiaoqing ; He, Hua ; Gao, Zhengliang
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descriptionStem cell-based therapies have been used for repairing damaged brain tissue and helping functional recovery after brain injury. Aberrance neurogenesis is related with brain injury, and multipotential neural stem cells from human embryonic stem (hES) cells provide a great promise for cell replacement therapies. Optimized protocols for neural differentiation are necessary to produce functional human neural stem cells (hNSCs) for cell therapy. However, the qualified procedure is scarce and detailed features of hNSCs originated from hES cells are still unclear. In this study, we developed a method to obtain hNSCs from hES cells, by which we could harvest abundant hNSCs in a relatively short time. Then, we examined the expression of pluripotent and multipotent marker genes through immunostaining and confirmed differentiation potential of the differentiated hNSCs. Furthermore, we analyzed the mitotic activity of these hNSCs. In this report, we provided comprehensive features of hNSCs and delivered the knowledge about how to obtain more high-quality hNSCs from hES cells which may help to accelerate the NSC-based therapies in brain injury treatment.
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titleEfficient and Fast Differentiation of Human Neural Stem Cells from Human Embryonic Stem Cells for Cell Therapy
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abstractStem cell-based therapies have been used for repairing damaged brain tissue and helping functional recovery after brain injury. Aberrance neurogenesis is related with brain injury, and multipotential neural stem cells from human embryonic stem (hES) cells provide a great promise for cell replacement therapies. Optimized protocols for neural differentiation are necessary to produce functional human neural stem cells (hNSCs) for cell therapy. However, the qualified procedure is scarce and detailed features of hNSCs originated from hES cells are still unclear. In this study, we developed a method to obtain hNSCs from hES cells, by which we could harvest abundant hNSCs in a relatively short time. Then, we examined the expression of pluripotent and multipotent marker genes through immunostaining and confirmed differentiation potential of the differentiated hNSCs. Furthermore, we analyzed the mitotic activity of these hNSCs. In this report, we provided comprehensive features of hNSCs and delivered the knowledge about how to obtain more high-quality hNSCs from hES cells which may help to accelerate the NSC-based therapies in brain injury treatment.
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