schliessen

Filtern

 

Bibliotheken

Analysis of Liver Transcriptome in Broilers with Ascites and Regulation by L-Carnitine

Two experiments were conducted using male Ross-308 broilers. Exp. 1 was to determine the differences in liver transcriptomic profile of healthy broilers at 39 days (D39) vs. 21 days of age (D21), and of ascites vs. healthy broilers at D39 by GeneChips, under a low-temperature environment. Differenti... Full description

Journal Title: The Journal of Poultry Science 2013, Vol.50(2), pp.126-137
Main Author: Wang, Yongwei
Other Authors: Guo, Yuming , Ning, Dong , Peng, Yunzhi , Yang, Ying , Liu, Dan
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 1346-7395 ; DOI: 10.2141/jpsa.0120124
Link: https://jlc.jst.go.jp/DN/JALC/10017625387?from=PrimoCentral
Zum Text:
SendSend as email Add to Book BagAdd to Book Bag
Staff View
recordid: jstageDN/JALC/10017625387
title: Analysis of Liver Transcriptome in Broilers with Ascites and Regulation by L-Carnitine
format: Article
creator:
  • Wang, Yongwei
  • Guo, Yuming
  • Ning, Dong
  • Peng, Yunzhi
  • Yang, Ying
  • Liu, Dan
subjects:
  • Broiler Ascites
  • L-Carnitine
  • Nutritional Regulation
  • Transcriptome
ispartof: The Journal of Poultry Science, 2013, Vol.50(2), pp.126-137
description: Two experiments were conducted using male Ross-308 broilers. Exp. 1 was to determine the differences in liver transcriptomic profile of healthy broilers at 39 days (D39) vs. 21 days of age (D21), and of ascites vs. healthy broilers at D39 by GeneChips, under a low-temperature environment. Differentially expressed genes related to liver antioxidative capacity and energy metabolism were screened. Exp. 2 was to analyze the regulatory roles of L-carnitine on genes and biochemical parameters involved in liver oxidoreduction and energy metabolism. The results showed that the expression of 790 genes in D39 vs D21 healthy broilers (386 up- and 404 down-regulated) and expression of 178 genes in ascites vs. healthy broilers on D39 (128 up- and 50 down-regulated) was changed. The analysis of pathway and GO indicated that genes related to amino acid metabolism, fatty acid metabolism, Krebs cycle, carbohydrate metabolism, vitamin metabolism, cell apoptosis, immune response, oxidoreduction, angiogenesis, nitric oxide formation and erythrocyte differentiation were involved in the development of ascites. Liver MDA level of ascites broilers was significantly increased, but the activity of T-SOD was significantly decreased than healthy broilers. The activity of liver HK, MDH, PK and Na+-K+-ATPase was significantly lower in ascites broilers. The expression of liver HKDC1 and PCK1 was significantly down-regulated, and that of HNF-4α and AKR1B10 expression was significantly up-regulated. L-carnitine supplementation significantly increased the activity of T-SOD and PK, and significantly up-regulated HKDC1 and HNF-4α expression. It can be concluded that genes involved in many biological processes are differentially expressed due to ascites. Liver oxidation damage and energy generation obstruction were found in broilers with ascites. L-carnitine can alleviate the liver oxidative damage and promote the generation and utilization of energy. The present results demonstrated that L-carnitine could serve as a potential regulatory agent to reduce ascites susceptibility and mortality.
language: eng
source:
identifier: ISSN: 1346-7395 ; DOI: 10.2141/jpsa.0120124
fulltext: fulltext
issn:
  • 1346-7395
  • 13467395
url: Link


@attributes
ID160069074
RANK0.07
NO1
SEARCH_ENGINEprimo_central_multiple_fe
SEARCH_ENGINE_TYPEPrimo Central Search Engine
LOCALfalse
PrimoNMBib
record
control
sourcerecordidDN/JALC/10017625387
sourceidjstage
recordidTN_jstageDN/JALC/10017625387
sourcesystemOther
display
typearticle
titleAnalysis of Liver Transcriptome in Broilers with Ascites and Regulation by L-Carnitine
creatorWang, Yongwei ; Guo, Yuming ; Ning, Dong ; Peng, Yunzhi ; Yang, Ying ; Liu, Dan
ispartofThe Journal of Poultry Science, 2013, Vol.50(2), pp.126-137
identifierISSN: 1346-7395 ; DOI: 10.2141/jpsa.0120124
subjectBroiler Ascites ; L-Carnitine ; Nutritional Regulation ; Transcriptome
descriptionTwo experiments were conducted using male Ross-308 broilers. Exp. 1 was to determine the differences in liver transcriptomic profile of healthy broilers at 39 days (D39) vs. 21 days of age (D21), and of ascites vs. healthy broilers at D39 by GeneChips, under a low-temperature environment. Differentially expressed genes related to liver antioxidative capacity and energy metabolism were screened. Exp. 2 was to analyze the regulatory roles of L-carnitine on genes and biochemical parameters involved in liver oxidoreduction and energy metabolism. The results showed that the expression of 790 genes in D39 vs D21 healthy broilers (386 up- and 404 down-regulated) and expression of 178 genes in ascites vs. healthy broilers on D39 (128 up- and 50 down-regulated) was changed. The analysis of pathway and GO indicated that genes related to amino acid metabolism, fatty acid metabolism, Krebs cycle, carbohydrate metabolism, vitamin metabolism, cell apoptosis, immune response, oxidoreduction, angiogenesis, nitric oxide formation and erythrocyte differentiation were involved in the development of ascites. Liver MDA level of ascites broilers was significantly increased, but the activity of T-SOD was significantly decreased than healthy broilers. The activity of liver HK, MDH, PK and Na+-K+-ATPase was significantly lower in ascites broilers. The expression of liver HKDC1 and PCK1 was significantly down-regulated, and that of HNF-4α and AKR1B10 expression was significantly up-regulated. L-carnitine supplementation significantly increased the activity of T-SOD and PK, and significantly up-regulated HKDC1 and HNF-4α expression. It can be concluded that genes involved in many biological processes are differentially expressed due to ascites. Liver oxidation damage and energy generation obstruction were found in broilers with ascites. L-carnitine can alleviate the liver oxidative damage and promote the generation and utilization of energy. The present results demonstrated that L-carnitine could serve as a potential regulatory agent to reduce ascites susceptibility and mortality.
languageeng
source
version3
lds50peer_reviewed
links
openurl$$Topenurl_article
backlink$$Uhttps://jlc.jst.go.jp/DN/JALC/10017625387?from=PrimoCentral$$EView_source_record
openurlfulltext$$Topenurlfull_article
search
creatorcontrib
0Wang, Yongwei
1Guo, Yuming
2Ning, Dong
3Peng, Yunzhi
4Yang, Ying
5Liu, Dan
titleAnalysis of Liver Transcriptome in Broilers with Ascites and Regulation by L-Carnitine
descriptionTwo experiments were conducted using male Ross-308 broilers. Exp. 1 was to determine the differences in liver transcriptomic profile of healthy broilers at 39 days (D39) vs. 21 days of age (D21), and of ascites vs. healthy broilers at D39 by GeneChips, under a low-temperature environment. Differentially expressed genes related to liver antioxidative capacity and energy metabolism were screened. Exp. 2 was to analyze the regulatory roles of L-carnitine on genes and biochemical parameters involved in liver oxidoreduction and energy metabolism. The results showed that the expression of 790 genes in D39 vs D21 healthy broilers (386 up- and 404 down-regulated) and expression of 178 genes in ascites vs. healthy broilers on D39 (128 up- and 50 down-regulated) was changed. The analysis of pathway and GO indicated that genes related to amino acid metabolism, fatty acid metabolism, Krebs cycle, carbohydrate metabolism, vitamin metabolism, cell apoptosis, immune response, oxidoreduction, angiogenesis, nitric oxide formation and erythrocyte differentiation were involved in the development of ascites. Liver MDA level of ascites broilers was significantly increased, but the activity of T-SOD was significantly decreased than healthy broilers. The activity of liver HK, MDH, PK and Na+-K+-ATPase was significantly lower in ascites broilers. The expression of liver HKDC1 and PCK1 was significantly down-regulated, and that of HNF-4α and AKR1B10 expression was significantly up-regulated. L-carnitine supplementation significantly increased the activity of T-SOD and PK, and significantly up-regulated HKDC1 and HNF-4α expression. It can be concluded that genes involved in many biological processes are differentially expressed due to ascites. Liver oxidation damage and energy generation obstruction were found in broilers with ascites. L-carnitine can alleviate the liver oxidative damage and promote the generation and utilization of energy. The present results demonstrated that L-carnitine could serve as a potential regulatory agent to reduce ascites susceptibility and mortality.
subject
0Broiler Ascites
1L-Carnitine
2Nutritional Regulation
3Transcriptome
general
0English
1Japan Poultry Science Association
2日本家禽学会
3J-STAGE (Japan Science and Technology Agency)
410.2141/jpsa.0120124
sourceidjstage
recordidjstageDN/JALC/10017625387
issn
01346-7395
113467395
rsrctypearticle
creationdate2013
addtitleThe Journal of Poultry Science
searchscopejstage
scopejstage
lsr30VSR-Enriched:[pages, eissn]
sort
titleAnalysis of Liver Transcriptome in Broilers with Ascites and Regulation by L-Carnitine
authorWang, Yongwei ; Guo, Yuming ; Ning, Dong ; Peng, Yunzhi ; Yang, Ying ; Liu, Dan
creationdate20130000
facets
frbrgroupid5363352945941724318
frbrtype5
newrecords20170214
languageeng
creationdate2013
topic
0Broiler Ascites
1L-Carnitine
2Nutritional Regulation
3Transcriptome
collectionJ-STAGE (Japan Science and Technology Agency)
prefilterarticles
rsrctypearticles
creatorcontrib
0Wang, Yongwei
1Guo, Yuming
2Ning, Dong
3Peng, Yunzhi
4Yang, Ying
5Liu, Dan
jtitle
0The Journal of Poultry Science
1Journal of Poultry Science
toplevelpeer_reviewed
delivery
delcategoryRemote Search Resource
fulltextfulltext
addata
aulastWang
aufirstYongwei
au
0Wang, Yongwei
1Guo, Yuming
2Ning, Dong
3Peng, Yunzhi
4Yang, Ying
5Liu, Dan
atitleAnalysis of Liver Transcriptome in Broilers with Ascites and Regulation by L-Carnitine
jtitleThe Journal of Poultry Science
date2013
risdate2013
volume50
issue2
spage126
epage137
issn1346-7395
genrearticle
ristypeJOUR
abstractTwo experiments were conducted using male Ross-308 broilers. Exp. 1 was to determine the differences in liver transcriptomic profile of healthy broilers at 39 days (D39) vs. 21 days of age (D21), and of ascites vs. healthy broilers at D39 by GeneChips, under a low-temperature environment. Differentially expressed genes related to liver antioxidative capacity and energy metabolism were screened. Exp. 2 was to analyze the regulatory roles of L-carnitine on genes and biochemical parameters involved in liver oxidoreduction and energy metabolism. The results showed that the expression of 790 genes in D39 vs D21 healthy broilers (386 up- and 404 down-regulated) and expression of 178 genes in ascites vs. healthy broilers on D39 (128 up- and 50 down-regulated) was changed. The analysis of pathway and GO indicated that genes related to amino acid metabolism, fatty acid metabolism, Krebs cycle, carbohydrate metabolism, vitamin metabolism, cell apoptosis, immune response, oxidoreduction, angiogenesis, nitric oxide formation and erythrocyte differentiation were involved in the development of ascites. Liver MDA level of ascites broilers was significantly increased, but the activity of T-SOD was significantly decreased than healthy broilers. The activity of liver HK, MDH, PK and Na+-K+-ATPase was significantly lower in ascites broilers. The expression of liver HKDC1 and PCK1 was significantly down-regulated, and that of HNF-4α and AKR1B10 expression was significantly up-regulated. L-carnitine supplementation significantly increased the activity of T-SOD and PK, and significantly up-regulated HKDC1 and HNF-4α expression. It can be concluded that genes involved in many biological processes are differentially expressed due to ascites. Liver oxidation damage and energy generation obstruction were found in broilers with ascites. L-carnitine can alleviate the liver oxidative damage and promote the generation and utilization of energy. The present results demonstrated that L-carnitine could serve as a potential regulatory agent to reduce ascites susceptibility and mortality.
pubJapan Poultry Science Association
doi10.2141/jpsa.0120124
pages126-137
eissn13490486