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Populations of Salmonella Enteritidis in the Tissues of Reproductive Organs in Laying Hens after Oral Challenge: A Real-Time PCR Study

The objective of this study was to determine the populations of Salmonella Enteritidis (S.Enteritidis) in reproductive organs of laying hens after oral challenge. We conducted serovar-specific real-time PCR for S.Enteritidis to detect the genomic DNA of S.Enteritidis from laying hens at different ti... Full description

Journal Title: The Journal of Poultry Science 2011, Vol.advpub
Main Author: He, Guang-Zhi
Other Authors: Tian, Wei-Yi , Deng, Shu-Xuan
Format: Electronic Article Electronic Article
Language: English
Subjects:
Pcr
&Lt
ID: ISSN: 1346-7395 ; DOI: 10.2141/jpsa.010133
Link: https://jlc.jst.go.jp/DN/JALC/90000002593?from=PrimoCentral
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recordid: jstageDN/JALC/90000002593
title: Populations of Salmonella Enteritidis in the Tissues of Reproductive Organs in Laying Hens after Oral Challenge: A Real-Time PCR Study
format: Article
creator:
  • He, Guang-Zhi
  • Tian, Wei-Yi
  • Deng, Shu-Xuan
subjects:
  • Population
  • Hens
  • Pcr
  • Reproductive Organs
  • &Lt
  • I&Gt
  • Salmonella&Lt
  • /I&Gt
  • Enteritidis
ispartof: The Journal of Poultry Science, 2011, Vol.advpub
description: The objective of this study was to determine the populations of Salmonella Enteritidis (S.Enteritidis) in reproductive organs of laying hens after oral challenge. We conducted serovar-specific real-time PCR for S.Enteritidis to detect the genomic DNA of S.Enteritidis from laying hens at different time points. To validate these results, the indirect fluorescent antibody (IFA) technique was employed too. The results showed that S.Enteritidis was consistently detected in all the samples. Vagina and uters was positive at 20 h PI, and the last organ to show a positive result was the largest and third largest preovulatory follicle, at 32 h PI. The copy numbers of S.Enteritidis DNA in each tissue reached a peak at 36 60 h PI, with the vagina and uterus containing higher concentrations than other tissues. However, the number of bacteria started decreasing by 3-4 d, and by 6 d, the concentration of S.Enteritidis DNA was below the detection limits of the PCR assay, except the Vagina. In conclusion, the results provided insights into the S.Enteritidis populations in the reproductive organs; This study will help in understanding the pathogenesis of S.Enteritidis infection in vivo.
language: eng
source:
identifier: ISSN: 1346-7395 ; DOI: 10.2141/jpsa.010133
fulltext: fulltext
issn:
  • 1346-7395
  • 13467395
url: Link


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