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Expression of GFP Gene in Gonads of Chicken Embryos by Transfecting Primordial Germ Cells in vitro or in vivo using the PiggyBac Transposon Vector System

In the present study, chicken primordial germ cells (PGCs) were transfected with GFP gene in vitro or in vivo using the piggyBac transposon vector system. PGCs cultured for 465 days were transfected in vitro, and GFP gene expression was observed in 25% of the treated PGCs after culturing for further... Full description

Journal Title: The Journal of Poultry Science 2015, Vol.52(3), pp.227-231
Main Author: Naito, Mitsuru
Other Authors: Harumi, Takashi , Kuwana, Takashi
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 1346-7395 ; DOI: 10.2141/jpsa.0140197
Link: https://jlc.jst.go.jp/DN/JLC/20013456403?from=PrimoCentral
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recordid: jstageDN/JLC/20013456403
title: Expression of GFP Gene in Gonads of Chicken Embryos by Transfecting Primordial Germ Cells in vitro or in vivo using the PiggyBac Transposon Vector System
format: Article
creator:
  • Naito, Mitsuru
  • Harumi, Takashi
  • Kuwana, Takashi
subjects:
  • Chick Embryo
  • Gfp Gene
  • Primordial Germ Cells
  • Transfection
  • Transposon
ispartof: The Journal of Poultry Science, 2015, Vol.52(3), pp.227-231
description: In the present study, chicken primordial germ cells (PGCs) were transfected with GFP gene in vitro or in vivo using the piggyBac transposon vector system. PGCs cultured for 465 days were transfected in vitro, and GFP gene expression was observed in 25% of the treated PGCs after culturing for further 42 days. The cultured PGCs expressing GFP gene were transferred to recipient embryos and strong GFP gene expression was observed in the recipient gonads at day 18.5 of incubation. Circulating PGCs were transfected in vivo, and intense GFP gene expression was observed in the gonads of recipient embryos at day 18.5 of incubation. The procedure employed in the present study will contribute to successful gene transfer into chickens.
language: eng
source:
identifier: ISSN: 1346-7395 ; DOI: 10.2141/jpsa.0140197
fulltext: fulltext
issn:
  • 1346-7395
  • 13467395
url: Link


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titleExpression of GFP Gene in Gonads of Chicken Embryos by Transfecting Primordial Germ Cells in vitro or in vivo using the PiggyBac Transposon Vector System
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ispartofThe Journal of Poultry Science, 2015, Vol.52(3), pp.227-231
identifierISSN: 1346-7395 ; DOI: 10.2141/jpsa.0140197
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