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Regulation of Autophagy in Chick Myotubes: Effects of Insulin, Insulin-Like Growth Factor-I, and Amino Acids

Autophagy, an intracellular bulk protein degradation system in skeletal muscle, is increased under catabolic conditions resulting in muscle atrophy. This study aimed to investigate the effects of insulin, insulin-like growth factor (IGF)-I, and amino acids on autophagy (LC3-II content and expression... Full description

Journal Title: The Journal of Poultry Science 2018, Vol.55(4), pp.257-262
Main Author: Nakashima, Kazuki
Other Authors: Ishida, Aiko
Format: Electronic Article Electronic Article
Language: English
Subjects:
Lc3
ID: ISSN: 1346-7395 ; DOI: 10.2141/jpsa.0170196
Link: https://jlc.jst.go.jp/DN/JLC/20099532596?from=PrimoCentral
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recordid: jstageDN/JLC/20099532596
title: Regulation of Autophagy in Chick Myotubes: Effects of Insulin, Insulin-Like Growth Factor-I, and Amino Acids
format: Article
creator:
  • Nakashima, Kazuki
  • Ishida, Aiko
subjects:
  • Amino Acids
  • Autophagy
  • Chick Myotubes
  • Igf-I
  • Insulin
  • Lc3
ispartof: The Journal of Poultry Science, 2018, Vol.55(4), pp.257-262
description: Autophagy, an intracellular bulk protein degradation system in skeletal muscle, is increased under catabolic conditions resulting in muscle atrophy. This study aimed to investigate the effects of insulin, insulin-like growth factor (IGF)-I, and amino acids on autophagy (LC3-II content and expression of autophagy-related genes) in chick myotubes. Chick myotubes were incubated with insulin (1 µg/ml), IGF-I (100 ng/ml), and amino acids for 3 h. The LC3-II content, an index of autophagosome formation, and mRNA expression of LC3B and GABARAPL1 were significantly decreased by insulin. The LC3-II content, but not mRNA expression of autophagy-related genes, was also significantly decreased by IGF-I. The LC3-II content and LC3B mRNA level were also significantly decreased by amino acids. The mRNA expression of atrogin-1/MAFbx, a muscle-specific ubiquitin ligase, was also significantly decreased by insulin, IGF-I, and amino acids in chick myotubes. These results indicated that insulin, IGF-I, and amino acids regulate autophagy as well as the ubiquitin–proteasome proteolytic pathway in chick myotubes.
language: eng
source:
identifier: ISSN: 1346-7395 ; DOI: 10.2141/jpsa.0170196
fulltext: fulltext
issn:
  • 1346-7395
  • 13467395
url: Link


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identifierISSN: 1346-7395 ; DOI: 10.2141/jpsa.0170196
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