schliessen

Filtern

 

Bibliotheken

ARTIFICIAL GENE-CLUSTERS ENGINEERED INTO PLANTS USING A VECTOR SYSTEM BASED ON INTRON-AND INTEIN-ENCODED ENDONUCLEASES

The ability to create artificial gene-clusters for genetic transformation could facilitate the development of crops with multiple engineered traits, or with traits which result from the expression of multiple genes. A simple method to assemble artificial gene-clusters was developed by designing a mu... Full description

Journal Title: In Vitro Cellular & Developmental Biology. Plant 1 November 2002, Vol.38(6), pp.537-542
Main Author: Thomson, J. Michael
Other Authors: Lafayette, Peter R. , Schmidt, Monica A. , Parrott, Wayne A.
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 10545476 ; E-ISSN: 14752689
Link: https://www.jstor.org/stable/41318704
Zum Text:
SendSend as email Add to Book BagAdd to Book Bag
Staff View
recordid: jstor_archive_2241318704
title: ARTIFICIAL GENE-CLUSTERS ENGINEERED INTO PLANTS USING A VECTOR SYSTEM BASED ON INTRON-AND INTEIN-ENCODED ENDONUCLEASES
format: Article
creator:
  • Thomson, J. Michael
  • Lafayette, Peter R.
  • Schmidt, Monica A.
  • Parrott, Wayne A.
subjects:
  • Biological sciences -- Biology -- Genetics
  • Applied sciences -- Engineering -- Aerospace engineering
  • Biological sciences -- Biology -- Botany
  • Applied sciences -- Food science -- Foodstuffs
  • Biological sciences -- Biology -- Genetics
  • Biological sciences -- Biology -- Genetics
  • Biological sciences -- Bioengineering -- Biotechnology
  • Physical sciences -- Chemistry -- Chemical compounds
  • Biological sciences -- Biology -- Genetics
  • Biological sciences -- Biology -- Genetics
ispartof: In Vitro Cellular & Developmental Biology. Plant, 1 November 2002, Vol.38(6), pp.537-542
description: The ability to create artificial gene-clusters for genetic transformation could facilitate the development of crops with multiple engineered traits, or with traits which result from the expression of multiple genes. A simple method to assemble artificial gene-clusters was developed by designing a multiple cloning site consisting of an array of homing endonuclease cleavage sites into a single vector. These enzymes are also known as intron-or intein-encoded endonucleases, and have very long recognition sequences, which makes them very rare cutters. The resulting vectors are pUGA for microprojectilemediated transformation, and pUGA2 for Agrooaciermm-mediated transformation. In addition, a series of unidirectional shuttle vectors containing various combinations of homing endonuclease restriction sites was constructed. Gene cassettes can be cloned into individual shuttles, and then transferred to either pUGA or pUGA2 to construct artificial gene-clusters. To test the feasibility of this approach, a six-gene cluster was constructed and transformed into soybean via microprojectile bombardment and into tobacco via Agrobacterium. The genes were assayed for expression in both the T₀ and T₁ generations for three independent transgenics. Up to five of the six genes were expressed. Additional changes to the construction of individual gene cassettes may improve the frequency with which all genes in the cluster are expressed.
language: eng
source:
identifier: ISSN: 10545476 ; E-ISSN: 14752689
fulltext: fulltext
issn:
  • 1054-5476
  • 10545476
  • 1475-2689
  • 14752689
url: Link


@attributes
ID1001865622
RANK0.06999999
NO1
SEARCH_ENGINEprimo_central_multiple_fe
SEARCH_ENGINE_TYPEPrimo Central Search Engine
LOCALfalse
PrimoNMBib
record
control
sourcerecordid41318704
sourceidjstor_archive_22
recordidTN_jstor_archive_2241318704
sourceformatXML
sourcesystemPC
pqid18676771
display
typearticle
titleARTIFICIAL GENE-CLUSTERS ENGINEERED INTO PLANTS USING A VECTOR SYSTEM BASED ON INTRON-AND INTEIN-ENCODED ENDONUCLEASES
creatorThomson, J. Michael ; Lafayette, Peter R. ; Schmidt, Monica A. ; Parrott, Wayne A.
ispartofIn Vitro Cellular & Developmental Biology. Plant, 1 November 2002, Vol.38(6), pp.537-542
identifierISSN: 10545476 ; E-ISSN: 14752689
subjectBiological sciences -- Biology -- Genetics ; Applied sciences -- Engineering -- Aerospace engineering ; Biological sciences -- Biology -- Botany ; Applied sciences -- Food science -- Foodstuffs ; Biological sciences -- Biology -- Genetics ; Biological sciences -- Biology -- Genetics ; Biological sciences -- Bioengineering -- Biotechnology ; Physical sciences -- Chemistry -- Chemical compounds ; Biological sciences -- Biology -- Genetics ; Biological sciences -- Biology -- Genetics
descriptionThe ability to create artificial gene-clusters for genetic transformation could facilitate the development of crops with multiple engineered traits, or with traits which result from the expression of multiple genes. A simple method to assemble artificial gene-clusters was developed by designing a multiple cloning site consisting of an array of homing endonuclease cleavage sites into a single vector. These enzymes are also known as intron-or intein-encoded endonucleases, and have very long recognition sequences, which makes them very rare cutters. The resulting vectors are pUGA for microprojectilemediated transformation, and pUGA2 for Agrooaciermm-mediated transformation. In addition, a series of unidirectional shuttle vectors containing various combinations of homing endonuclease restriction sites was constructed. Gene cassettes can be cloned into individual shuttles, and then transferred to either pUGA or pUGA2 to construct artificial gene-clusters. To test the feasibility of this approach, a six-gene cluster was constructed and transformed into soybean via microprojectile bombardment and into tobacco via Agrobacterium. The genes were assayed for expression in both the T₀ and T₁ generations for three independent transgenics. Up to five of the six genes were expressed. Additional changes to the construction of individual gene cassettes may improve the frequency with which all genes in the cluster are expressed.
languageeng
source
version5
lds50peer_reviewed
links
openurl$$Topenurl_article
backlink$$Uhttps://www.jstor.org/stable/41318704$$EView_this_record_in_JSTOR
openurlfulltext$$Topenurlfull_article
search
creatorcontrib
0THOMSON, J. MICHAEL
1LAFAYETTE, PETER R.
2SCHMIDT, MONICA A.
3PARROTT, WAYNE A.
titleARTIFICIAL GENE-CLUSTERS ENGINEERED INTO PLANTS USING A VECTOR SYSTEM BASED ON INTRON-AND INTEIN-ENCODED ENDONUCLEASES
descriptionThe ability to create artificial gene-clusters for genetic transformation could facilitate the development of crops with multiple engineered traits, or with traits which result from the expression of multiple genes. A simple method to assemble artificial gene-clusters was developed by designing a multiple cloning site consisting of an array of homing endonuclease cleavage sites into a single vector. These enzymes are also known as intron-or intein-encoded endonucleases, and have very long recognition sequences, which makes them very rare cutters. The resulting vectors are pUGA for microprojectilemediated transformation, and pUGA2 for Agrooaciermm-mediated transformation. In addition, a series of unidirectional shuttle vectors containing various combinations of homing endonuclease restriction sites was constructed. Gene cassettes can be cloned into individual shuttles, and then transferred to either pUGA or pUGA2 to construct artificial gene-clusters. To test the feasibility of this approach, a six-gene cluster was constructed and transformed into soybean via microprojectile bombardment and into tobacco via Agrobacterium. The genes were assayed for expression in both the T₀ and T₁ generations for three independent transgenics. Up to five of the six genes were expressed. Additional changes to the construction of individual gene cassettes may improve the frequency with which all genes in the cluster are expressed.
subject
0Biological sciences -- Biology -- Genetics
1Applied sciences -- Engineering -- Aerospace engineering
2Biological sciences -- Biology -- Botany
3Applied sciences -- Food science -- Foodstuffs
4Biological sciences -- Bioengineering -- Biotechnology
5Physical sciences -- Chemistry -- Chemical compounds
general
0English
1Springer
2Archival Journals (JSTOR)
3JSTOR Archival Journals
sourceidjstor_archive_22
recordidjstor_archive_2241318704
issn
01054-5476
110545476
21475-2689
314752689
rsrctypearticle
creationdate2002
recordtypearticle
addtitleIn Vitro Cellular & Developmental Biology. Plant
searchscope
0jstor
1jstor_ebc2
scope
0jstor
1jstor_ebc2
citationpf 537
startdate20021101
enddate20021101
lsr30VSR-Enriched:[pqid, doi]
sort
titleARTIFICIAL GENE-CLUSTERS ENGINEERED INTO PLANTS USING A VECTOR SYSTEM BASED ON INTRON-AND INTEIN-ENCODED ENDONUCLEASES
authorThomson, J. Michael ; Lafayette, Peter R. ; Schmidt, Monica A. ; Parrott, Wayne A.
creationdate20021101
lso0120021101
facets
frbrgroupid7579909342125628578
frbrtype5
languageeng
creationdate2002
topic
0Biological sciences -- Biology -- Genetics
1Applied sciences -- Engineering -- Aerospace engineering
2Biological sciences -- Biology -- Botany
3Applied sciences -- Food science -- Foodstuffs
4Biological sciences -- Bioengineering -- Biotechnology
5Physical sciences -- Chemistry -- Chemical compounds
collectionJSTOR Archival Journals
prefilterarticles
rsrctypearticles
creatorcontrib
0Thomson, J. Michael
1Lafayette, Peter R.
2Schmidt, Monica A.
3Parrott, Wayne A.
jtitleIn Vitro Cellular & Developmental Biology. Plant
toplevelpeer_reviewed
delivery
delcategoryRemote Search Resource
fulltextfulltext
addata
aulast
0Thomson
1Lafayette
2Schmidt
3Parrott
aufirst
0J. Michael
1Peter R.
2Monica A.
3Wayne A.
au
0Thomson, J. Michael
1Lafayette, Peter R.
2Schmidt, Monica A.
3Parrott, Wayne A.
atitleARTIFICIAL GENE-CLUSTERS ENGINEERED INTO PLANTS USING A VECTOR SYSTEM BASED ON INTRON-AND INTEIN-ENCODED ENDONUCLEASES
jtitleIn Vitro Cellular & Developmental Biology. Plant
risdate20021101
volume38
issue6
spage537
epage542
pages537-542
issn10545476
eissn14752689
formatjournal
genrearticle
ristypeJOUR
abstractThe ability to create artificial gene-clusters for genetic transformation could facilitate the development of crops with multiple engineered traits, or with traits which result from the expression of multiple genes. A simple method to assemble artificial gene-clusters was developed by designing a multiple cloning site consisting of an array of homing endonuclease cleavage sites into a single vector. These enzymes are also known as intron-or intein-encoded endonucleases, and have very long recognition sequences, which makes them very rare cutters. The resulting vectors are pUGA for microprojectilemediated transformation, and pUGA2 for Agrooaciermm-mediated transformation. In addition, a series of unidirectional shuttle vectors containing various combinations of homing endonuclease restriction sites was constructed. Gene cassettes can be cloned into individual shuttles, and then transferred to either pUGA or pUGA2 to construct artificial gene-clusters. To test the feasibility of this approach, a six-gene cluster was constructed and transformed into soybean via microprojectile bombardment and into tobacco via Agrobacterium. The genes were assayed for expression in both the T₀ and T₁ generations for three independent transgenics. Up to five of the six genes were expressed. Additional changes to the construction of individual gene cassettes may improve the frequency with which all genes in the cluster are expressed.
pubSpringer
doi10.1079/IVP2002329
date2002-11-01