schliessen

Filtern

 

Bibliotheken

Molecular cloning and genomic organization of mouse homologue of Drosophila germ cell-less and its expression in germ lineage cells

Primordial germ cells (PGCs) are founder cells of all gametes. A number of genes which control PGCs development have been identified in invertebrates, whereas such genes are by and large unelucidated in mammals. Here we describe cloning, genomic structure and expression of mouse homologue of germ ce... Full description

Journal Title: Biochemical and biophysical research communications 19 August 1999, Vol.262(1), pp.223-30
Main Author: Kimura, T
Other Authors: Yomogida, K , Iwai, N , Kato, Y , Nakano, T
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 0006-291X ; PMID: 10448096 Version:1
Link: http://pubmed.gov/10448096
Zum Text:
SendSend as email Add to Book BagAdd to Book Bag
Staff View
recordid: medline10448096
title: Molecular cloning and genomic organization of mouse homologue of Drosophila germ cell-less and its expression in germ lineage cells
format: Article
creator:
  • Kimura, T
  • Yomogida, K
  • Iwai, N
  • Kato, Y
  • Nakano, T
subjects:
  • Drosophila Proteins
  • Gene Expression
  • Cell Lineage -- Genetics
  • Germ Cells -- Metabolism
  • Nuclear Proteins -- Genetics
  • Stem Cells -- Metabolism
ispartof: Biochemical and biophysical research communications, 19 August 1999, Vol.262(1), pp.223-30
description: Primordial germ cells (PGCs) are founder cells of all gametes. A number of genes which control PGCs development have been identified in invertebrates, whereas such genes are by and large unelucidated in mammals. Here we describe cloning, genomic structure and expression of mouse homologue of germ cell-less (gcl) gene which is required for PGCs formation in Drosophila. The mouse gcl shows 34% identity compared with Drosophila gcl protein and contains BTB/POZ domain. The gcl gene consists of 14 exons and spans more than 50 kb. The CpG islands are found around exon 1 of the gene. Putative promoter region contains potential binding sites for various transcription factors. Northern blot analysis showed that its mRNA is highly expressed in adult testis with lower expression in ovary, ES (embryonic stem) cells, and various other organs. In situ hybridization analysis revealed strong expression of the gcl gene in the pachytene stage spermatocytes. The expression was also observed in post-migratory...
language: eng
source:
identifier: ISSN: 0006-291X ; PMID: 10448096 Version:1
fulltext: fulltext
issn:
  • 0006291X
  • 0006-291X
url: Link


@attributes
ID26473910
RANK0.07
NO1
SEARCH_ENGINEprimo_central_multiple_fe
SEARCH_ENGINE_TYPEPrimo Central Search Engine
LOCALfalse
PrimoNMBib
record
control
sourcerecordid10448096
sourceidmedline
recordidTN_medline10448096
sourceformatXML
sourcesystemPC
pqid17313830
ingidap/rc/1999/00000262/00000001/art01160
display
typearticle
titleMolecular cloning and genomic organization of mouse homologue of Drosophila germ cell-less and its expression in germ lineage cells
creatorKimura, T ; Yomogida, K ; Iwai, N ; Kato, Y ; Nakano, T
ispartofBiochemical and biophysical research communications, 19 August 1999, Vol.262(1), pp.223-30
identifierISSN: 0006-291X ; PMID: 10448096 Version:1
subjectDrosophila Proteins ; Gene Expression ; Cell Lineage -- Genetics ; Germ Cells -- Metabolism ; Nuclear Proteins -- Genetics ; Stem Cells -- Metabolism
descriptionPrimordial germ cells (PGCs) are founder cells of all gametes. A number of genes which control PGCs development have been identified in invertebrates, whereas such genes are by and large unelucidated in mammals. Here we describe cloning, genomic structure and expression of mouse homologue of germ cell-less (gcl) gene which is required for PGCs formation in Drosophila. The mouse gcl shows 34% identity compared with Drosophila gcl protein and contains BTB/POZ domain. The gcl gene consists of 14 exons and spans more than 50 kb. The CpG islands are found around exon 1 of the gene. Putative promoter region contains potential binding sites for various transcription factors. Northern blot analysis showed that its mRNA is highly expressed in adult testis with lower expression in ovary, ES (embryonic stem) cells, and various other organs. In situ hybridization analysis revealed strong expression of the gcl gene in the pachytene stage spermatocytes. The expression was also observed in post-migratory...
languageeng
source
version7
lds50peer_reviewed
links
openurl$$Topenurl_article
backlink$$Uhttp://pubmed.gov/10448096$$EView_this_record_in_MEDLINE/PubMed
openurlfulltext$$Topenurlfull_article
addlink$$Uhttp://exlibris-pub.s3.amazonaws.com/aboutMedline.html$$EView_the_MEDLINE/PubMed_Copyright_Statement
search
creatorcontrib
0Kimura, T
1Yomogida, K
2Iwai, N
3Kato, Y
4Nakano, T
titleMolecular cloning and genomic organization of mouse homologue of Drosophila germ cell-less and its expression in germ lineage cells
descriptionPrimordial germ cells (PGCs) are founder cells of all gametes. A number of genes which control PGCs development have been identified in invertebrates, whereas such genes are by and large unelucidated in mammals. Here we describe cloning, genomic structure and expression of mouse homologue of germ cell-less (gcl) gene which is required for PGCs formation in Drosophila. The mouse gcl shows 34% identity compared with Drosophila gcl protein and contains BTB/POZ domain. The gcl gene consists of 14 exons and spans more than 50 kb. The CpG islands are found around exon 1 of the gene. Putative promoter region contains potential binding sites for various transcription factors. Northern blot analysis showed that its mRNA is highly expressed in adult testis with lower expression in ovary, ES (embryonic stem) cells, and various other organs. In situ hybridization analysis revealed strong expression of the gcl gene in the pachytene stage spermatocytes. The expression was also observed in post-migratory...
subject
0Drosophila Proteins
1Gene Expression
2Cell Lineage -- Genetics
3Germ Cells -- Metabolism
4Nuclear Proteins -- Genetics
5Stem Cells -- Metabolism
general
010448096
1English
2MEDLINE/PubMed (U.S. National Library of Medicine)
3MEDLINE/PubMed (NLM)
sourceidmedline
recordidmedline10448096
issn
00006291X
10006-291X
rsrctypearticle
creationdate1999
addtitleBiochemical and biophysical research communications
searchscope
0medline
1nlm_medline
2MEDLINE
scope
0medline
1nlm_medline
2MEDLINE
lsr4119990819
citationpf 223 vol 262 issue 1
startdate19990819
enddate19990819
lsr30VSR-Enriched:[eissn, doi, pqid, ingid, pages]
sort
titleMolecular cloning and genomic organization of mouse homologue of Drosophila germ cell-less and its expression in germ lineage cells
authorKimura, T ; Yomogida, K ; Iwai, N ; Kato, Y ; Nakano, T
creationdate19990819
lso0119990819
facets
frbrgroupid7862616217962712349
frbrtype5
newrecords20190701
languageeng
creationdate1999
topic
0Drosophila Proteins
1Gene Expression
2Cell Lineage–Genetics
3Germ Cells–Metabolism
4Nuclear Proteins–Genetics
5Stem Cells–Metabolism
collectionMEDLINE/PubMed (NLM)
prefilterarticles
rsrctypearticles
creatorcontrib
0Kimura, T
1Yomogida, K
2Iwai, N
3Kato, Y
4Nakano, T
jtitleBiochemical And Biophysical Research Communications
toplevelpeer_reviewed
delivery
delcategoryRemote Search Resource
fulltextfulltext
addata
aulast
0Kimura
1Yomogida
2Iwai
3Kato
4Nakano
aufirst
0T
1K
2N
3Y
au
0Kimura, T
1Yomogida, K
2Iwai, N
3Kato, Y
4Nakano, T
atitleMolecular cloning and genomic organization of mouse homologue of Drosophila germ cell-less and its expression in germ lineage cells
jtitleBiochemical and biophysical research communications
risdate19990819
volume262
issue1
spage223
pages223-230
issn0006-291X
formatjournal
genrearticle
ristypeJOUR
abstractPrimordial germ cells (PGCs) are founder cells of all gametes. A number of genes which control PGCs development have been identified in invertebrates, whereas such genes are by and large unelucidated in mammals. Here we describe cloning, genomic structure and expression of mouse homologue of germ cell-less (gcl) gene which is required for PGCs formation in Drosophila. The mouse gcl shows 34% identity compared with Drosophila gcl protein and contains BTB/POZ domain. The gcl gene consists of 14 exons and spans more than 50 kb. The CpG islands are found around exon 1 of the gene. Putative promoter region contains potential binding sites for various transcription factors. Northern blot analysis showed that its mRNA is highly expressed in adult testis with lower expression in ovary, ES (embryonic stem) cells, and various other organs. In situ hybridization analysis revealed strong expression of the gcl gene in the pachytene stage spermatocytes. The expression was also observed in post-migratory...
pmid10448096
doi10.1006/bbrc.1999.1160
eissn10902104
date1999-08-19