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MR molecular imaging and fluorescence microscopy for identification of activated tumor endothelium using a bimodal lipidic nanoparticle

In oncological research, there is a great need for imaging techniques that specifically identify angiogenic blood vessels in tumors on the basis of differences in the expression level of biomolecular markers. In the angiogenic cascade, different cell surface receptors, including the alphavbeta3-inte... Full description

Journal Title: FASEB journal : official publication of the Federation of American Societies for Experimental Biology December 2005, Vol.19(14), pp.2008-10
Main Author: Mulder, Willem J M
Other Authors: Strijkers, Gustav J , Habets, Jo W , Bleeker, Egbert J W , Van Der Schaft, Daisy W J , Storm, Gert , Koning, Gerben A , Griffioen, Arjan W , Nicolay, Klaas
Format: Electronic Article Electronic Article
Language: English
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ID: E-ISSN: 1530-6860 ; PMID: 16204353 Version:1
Link: http://pubmed.gov/16204353
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recordid: medline16204353
title: MR molecular imaging and fluorescence microscopy for identification of activated tumor endothelium using a bimodal lipidic nanoparticle
format: Article
creator:
  • Mulder, Willem J M
  • Strijkers, Gustav J
  • Habets, Jo W
  • Bleeker, Egbert J W
  • Van Der Schaft, Daisy W J
  • Storm, Gert
  • Koning, Gerben A
  • Griffioen, Arjan W
  • Nicolay, Klaas
subjects:
  • Nanostructures
  • Lipids -- Chemistry
  • Magnetic Resonance Imaging -- Methods
  • Microscopy, Fluorescence -- Methods
  • Neoplasms -- Metabolism
ispartof: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, December 2005, Vol.19(14), pp.2008-10
description: In oncological research, there is a great need for imaging techniques that specifically identify angiogenic blood vessels in tumors on the basis of differences in the expression level of biomolecular markers. In the angiogenic cascade, different cell surface receptors, including the alphavbeta3-integrin, are strongly expressed on activated endothelial cells. In the present study, we aimed to image angiogenesis by detecting the expression of alphavbeta3 in tumor bearing mice with a combination of magnetic resonance imaging (MRI) and fluorescence microscopy. To that end, we prepared MR-detectable and fluorescent liposomes, which carry approximately 700 alphavbeta3-specific RGD peptides per liposome. RGD competition experiments and RAD-conjugated liposomes were used as controls for specificity. In vivo, both RAD liposomes and RGD liposomes gave rise to signal increase on T1-weighted MR images. It was established by the use of ex vivo fluorescence microscopy that RGD liposomes and RAD liposomes...
language: eng
source:
identifier: E-ISSN: 1530-6860 ; PMID: 16204353 Version:1
fulltext: fulltext
issn:
  • 15306860
  • 1530-6860
url: Link


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titleMR molecular imaging and fluorescence microscopy for identification of activated tumor endothelium using a bimodal lipidic nanoparticle
creatorMulder, Willem J M ; Strijkers, Gustav J ; Habets, Jo W ; Bleeker, Egbert J W ; Van Der Schaft, Daisy W J ; Storm, Gert ; Koning, Gerben A ; Griffioen, Arjan W ; Nicolay, Klaas
ispartofFASEB journal : official publication of the Federation of American Societies for Experimental Biology, December 2005, Vol.19(14), pp.2008-10
identifierE-ISSN: 1530-6860 ; PMID: 16204353 Version:1
subjectNanostructures ; Lipids -- Chemistry ; Magnetic Resonance Imaging -- Methods ; Microscopy, Fluorescence -- Methods ; Neoplasms -- Metabolism
descriptionIn oncological research, there is a great need for imaging techniques that specifically identify angiogenic blood vessels in tumors on the basis of differences in the expression level of biomolecular markers. In the angiogenic cascade, different cell surface receptors, including the alphavbeta3-integrin, are strongly expressed on activated endothelial cells. In the present study, we aimed to image angiogenesis by detecting the expression of alphavbeta3 in tumor bearing mice with a combination of magnetic resonance imaging (MRI) and fluorescence microscopy. To that end, we prepared MR-detectable and fluorescent liposomes, which carry approximately 700 alphavbeta3-specific RGD peptides per liposome. RGD competition experiments and RAD-conjugated liposomes were used as controls for specificity. In vivo, both RAD liposomes and RGD liposomes gave rise to signal increase on T1-weighted MR images. It was established by the use of ex vivo fluorescence microscopy that RGD liposomes and RAD liposomes...
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titleMR molecular imaging and fluorescence microscopy for identification of activated tumor endothelium using a bimodal lipidic nanoparticle
descriptionIn oncological research, there is a great need for imaging techniques that specifically identify angiogenic blood vessels in tumors on the basis of differences in the expression level of biomolecular markers. In the angiogenic cascade, different cell surface receptors, including the alphavbeta3-integrin, are strongly expressed on activated endothelial cells. In the present study, we aimed to image angiogenesis by detecting the expression of alphavbeta3 in tumor bearing mice with a combination of magnetic resonance imaging (MRI) and fluorescence microscopy. To that end, we prepared MR-detectable and fluorescent liposomes, which carry approximately 700 alphavbeta3-specific RGD peptides per liposome. RGD competition experiments and RAD-conjugated liposomes were used as controls for specificity. In vivo, both RAD liposomes and RGD liposomes gave rise to signal increase on T1-weighted MR images. It was established by the use of ex vivo fluorescence microscopy that RGD liposomes and RAD liposomes...
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titleMR molecular imaging and fluorescence microscopy for identification of activated tumor endothelium using a bimodal lipidic nanoparticle
authorMulder, Willem J M ; Strijkers, Gustav J ; Habets, Jo W ; Bleeker, Egbert J W ; Van Der Schaft, Daisy W J ; Storm, Gert ; Koning, Gerben A ; Griffioen, Arjan W ; Nicolay, Klaas
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abstractIn oncological research, there is a great need for imaging techniques that specifically identify angiogenic blood vessels in tumors on the basis of differences in the expression level of biomolecular markers. In the angiogenic cascade, different cell surface receptors, including the alphavbeta3-integrin, are strongly expressed on activated endothelial cells. In the present study, we aimed to image angiogenesis by detecting the expression of alphavbeta3 in tumor bearing mice with a combination of magnetic resonance imaging (MRI) and fluorescence microscopy. To that end, we prepared MR-detectable and fluorescent liposomes, which carry approximately 700 alphavbeta3-specific RGD peptides per liposome. RGD competition experiments and RAD-conjugated liposomes were used as controls for specificity. In vivo, both RAD liposomes and RGD liposomes gave rise to signal increase on T1-weighted MR images. It was established by the use of ex vivo fluorescence microscopy that RGD liposomes and RAD liposomes...
pmid16204353
doi10.1096/fj.05-4145fje
date2005-12