schliessen

Filtern

 

Bibliotheken

Mesenchymal stem cells enhance allogeneic islet engraftment in nonhuman primates

To test the graft-promoting effects of mesenchymal stem cells (MSCs) in a cynomolgus monkey model of islet/bone marrow transplantation. Cynomolgus MSCs were obtained from iliac crest aspirate and characterized through passage 11 for phenotype, gene expression, differentiation potential, and karyotyp... Full description

Journal Title: Diabetes October 2010, Vol.59(10), pp.2558-68
Main Author: Berman, Dora M
Other Authors: Willman, Melissa A , Han, Dongmei , Kleiner, Gary , Kenyon, Norman M , Cabrera, Over , Karl, Julie A , Wiseman, Roger W , O'Connor, David H , Bartholomew, Amelia M , Kenyon, Norma S
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: E-ISSN: 1939-327X ; PMID: 20622174 Version:1 ; DOI: 10.2337/db10-0136
Link: http://pubmed.gov/20622174
Zum Text:
SendSend as email Add to Book BagAdd to Book Bag
Staff View
recordid: medline20622174
title: Mesenchymal stem cells enhance allogeneic islet engraftment in nonhuman primates
format: Article
creator:
  • Berman, Dora M
  • Willman, Melissa A
  • Han, Dongmei
  • Kleiner, Gary
  • Kenyon, Norman M
  • Cabrera, Over
  • Karl, Julie A
  • Wiseman, Roger W
  • O'Connor, David H
  • Bartholomew, Amelia M
  • Kenyon, Norma S
subjects:
  • Diabetes Mellitus, Experimental -- Surgery
  • Islets of Langerhans Transplantation -- Physiology
  • Mesenchymal Stem Cell Transplantation -- Methods
ispartof: Diabetes, October 2010, Vol.59(10), pp.2558-68
description: To test the graft-promoting effects of mesenchymal stem cells (MSCs) in a cynomolgus monkey model of islet/bone marrow transplantation. Cynomolgus MSCs were obtained from iliac crest aspirate and characterized through passage 11 for phenotype, gene expression, differentiation potential, and karyotype. Allogeneic donor MSCs were cotransplanted intraportally with islets on postoperative day (POD) 0 and intravenously with donor marrow on PODs 5 and 11. Recipients were followed for stabilization of blood glucose levels, reduction of exogenous insulin requirement (EIR), C-peptide levels, changes in peripheral blood T regulatory cells, and chimerism. Destabilization of glycemia and increases in EIR were used as signs of rejection; additional intravenous MSCs were administered to test the effect on reversal of rejection. MSC phenotype and a normal karyotype were observed through passage 11. IL-6, IL-10, vascular endothelial growth factor, TGF-β, hepatocyte growth factor, and galectin-1 gene expression levels varied among donors. MSC treatment significantly enhanced islet engraftment and function at 1 month posttransplant (n = 8), as compared with animals that received islets without MSCs (n = 3). Additional infusions of donor or third-party MSCs resulted in reversal of rejection episodes and prolongation of islet function in two animals. Stable islet allograft function was associated with increased numbers of regulatory T-cells in peripheral blood. MSCs may provide an important approach for enhancement of islet engraftment, thereby decreasing the numbers of islets needed to achieve insulin independence. Furthermore, MSCs may serve as a new, safe, and effective antirejection therapy.
language: eng
source:
identifier: E-ISSN: 1939-327X ; PMID: 20622174 Version:1 ; DOI: 10.2337/db10-0136
fulltext: fulltext
issn:
  • 1939327X
  • 1939-327X
url: Link


@attributes
ID1509373191
RANK0.07
NO1
SEARCH_ENGINEprimo_central_multiple_fe
SEARCH_ENGINE_TYPEPrimo Central Search Engine
LOCALfalse
PrimoNMBib
record
control
sourcerecordid20622174
sourceidmedline
recordidTN_medline20622174
sourceformatXML
sourcesystemOther
pqid755968145
display
typearticle
titleMesenchymal stem cells enhance allogeneic islet engraftment in nonhuman primates
creatorBerman, Dora M ; Willman, Melissa A ; Han, Dongmei ; Kleiner, Gary ; Kenyon, Norman M ; Cabrera, Over ; Karl, Julie A ; Wiseman, Roger W ; O'Connor, David H ; Bartholomew, Amelia M ; Kenyon, Norma S
ispartofDiabetes, October 2010, Vol.59(10), pp.2558-68
identifier
subjectDiabetes Mellitus, Experimental -- Surgery ; Islets of Langerhans Transplantation -- Physiology ; Mesenchymal Stem Cell Transplantation -- Methods
descriptionTo test the graft-promoting effects of mesenchymal stem cells (MSCs) in a cynomolgus monkey model of islet/bone marrow transplantation. Cynomolgus MSCs were obtained from iliac crest aspirate and characterized through passage 11 for phenotype, gene expression, differentiation potential, and karyotype. Allogeneic donor MSCs were cotransplanted intraportally with islets on postoperative day (POD) 0 and intravenously with donor marrow on PODs 5 and 11. Recipients were followed for stabilization of blood glucose levels, reduction of exogenous insulin requirement (EIR), C-peptide levels, changes in peripheral blood T regulatory cells, and chimerism. Destabilization of glycemia and increases in EIR were used as signs of rejection; additional intravenous MSCs were administered to test the effect on reversal of rejection. MSC phenotype and a normal karyotype were observed through passage 11. IL-6, IL-10, vascular endothelial growth factor, TGF-β, hepatocyte growth factor, and galectin-1 gene expression levels varied among donors. MSC treatment significantly enhanced islet engraftment and function at 1 month posttransplant (n = 8), as compared with animals that received islets without MSCs (n = 3). Additional infusions of donor or third-party MSCs resulted in reversal of rejection episodes and prolongation of islet function in two animals. Stable islet allograft function was associated with increased numbers of regulatory T-cells in peripheral blood. MSCs may provide an important approach for enhancement of islet engraftment, thereby decreasing the numbers of islets needed to achieve insulin independence. Furthermore, MSCs may serve as a new, safe, and effective antirejection therapy.
languageeng
source
version7
lds50peer_reviewed
links
openurl$$Topenurl_article
backlink$$Uhttp://pubmed.gov/20622174$$EView_this_record_in_MEDLINE/PubMed
openurlfulltext$$Topenurlfull_article
addlink$$Uhttp://exlibris-pub.s3.amazonaws.com/aboutMedline.html$$EView_the_MEDLINE/PubMed_Copyright_Statement
search
creatorcontrib
0Berman, Dora M
1Willman, Melissa A
2Han, Dongmei
3Kleiner, Gary
4Kenyon, Norman M
5Cabrera, Over
6Karl, Julie A
7Wiseman, Roger W
8O'Connor, David H
9Bartholomew, Amelia M
10Kenyon, Norma S
titleMesenchymal stem cells enhance allogeneic islet engraftment in nonhuman primates
description
0To test the graft-promoting effects of mesenchymal stem cells (MSCs) in a cynomolgus monkey model of islet/bone marrow transplantation.
1Cynomolgus MSCs were obtained from iliac crest aspirate and characterized through passage 11 for phenotype, gene expression, differentiation potential, and karyotype. Allogeneic donor MSCs were cotransplanted intraportally with islets on postoperative day (POD) 0 and intravenously with donor marrow on PODs 5 and 11. Recipients were followed for stabilization of blood glucose levels, reduction of exogenous insulin requirement (EIR), C-peptide levels, changes in peripheral blood T regulatory cells, and chimerism. Destabilization of glycemia and increases in EIR were used as signs of rejection; additional intravenous MSCs were administered to test the effect on reversal of rejection.
2MSC phenotype and a normal karyotype were observed through passage 11. IL-6, IL-10, vascular endothelial growth factor, TGF-β, hepatocyte growth factor, and galectin-1 gene expression levels varied among donors. MSC treatment significantly enhanced islet engraftment and function at 1 month posttransplant (n = 8), as compared with animals that received islets without MSCs (n = 3). Additional infusions of donor or third-party MSCs resulted in reversal of rejection episodes and prolongation of islet function in two animals. Stable islet allograft function was associated with increased numbers of regulatory T-cells in peripheral blood.
3MSCs may provide an important approach for enhancement of islet engraftment, thereby decreasing the numbers of islets needed to achieve insulin independence. Furthermore, MSCs may serve as a new, safe, and effective antirejection therapy.
subject
0Diabetes Mellitus, Experimental -- Surgery
1Islets of Langerhans Transplantation -- Physiology
2Mesenchymal Stem Cell Transplantation -- Methods
general
020622174
1English
2MEDLINE/PubMed (U.S. National Library of Medicine)
310.2337/db10-0136
4MEDLINE/PubMed (NLM)
sourceidmedline
recordidmedline20622174
issn
01939327X
11939-327X
rsrctypearticle
creationdate2010
addtitleDiabetes
searchscope
0medline
1nlm_medline
2MEDLINE
scope
0medline
1nlm_medline
2MEDLINE
lsr41201010
citationpf 2558 vol 59 issue 10
startdate20101001
enddate20101031
lsr30VSR-Enriched:[pages, pqid, issn]
sort
titleMesenchymal stem cells enhance allogeneic islet engraftment in nonhuman primates
authorBerman, Dora M ; Willman, Melissa A ; Han, Dongmei ; Kleiner, Gary ; Kenyon, Norman M ; Cabrera, Over ; Karl, Julie A ; Wiseman, Roger W ; O'Connor, David H ; Bartholomew, Amelia M ; Kenyon, Norma S
creationdate20101000
lso0120101000
facets
frbrgroupid6252679664939024717
frbrtype5
newrecords20190701
languageeng
creationdate2010
topic
0Diabetes Mellitus, Experimental–Surgery
1Islets of Langerhans Transplantation–Physiology
2Mesenchymal Stem Cell Transplantation–Methods
collectionMEDLINE/PubMed (NLM)
prefilterarticles
rsrctypearticles
creatorcontrib
0Berman, Dora M
1Willman, Melissa A
2Han, Dongmei
3Kleiner, Gary
4Kenyon, Norman M
5Cabrera, Over
6Karl, Julie A
7Wiseman, Roger W
8O'Connor, David H
9Bartholomew, Amelia M
10Kenyon, Norma S
jtitleDiabetes
toplevelpeer_reviewed
delivery
delcategoryRemote Search Resource
fulltextfulltext
addata
aulast
0Berman
1Willman
2Han
3Kleiner
4Kenyon
5Cabrera
6Karl
7Wiseman
8O'Connor
9Bartholomew
aufirst
0Dora M
1Melissa A
2Dongmei
3Gary
4Norman M
5Over
6Julie A
7Roger W
8David H
9Amelia M
10Norma S
au
0Berman, Dora M
1Willman, Melissa A
2Han, Dongmei
3Kleiner, Gary
4Kenyon, Norman M
5Cabrera, Over
6Karl, Julie A
7Wiseman, Roger W
8O'Connor, David H
9Bartholomew, Amelia M
10Kenyon, Norma S
atitleMesenchymal stem cells enhance allogeneic islet engraftment in nonhuman primates
jtitleDiabetes
risdate201010
volume59
issue10
spage2558
pages2558-2568
eissn1939-327X
formatjournal
genrearticle
ristypeJOUR
abstractTo test the graft-promoting effects of mesenchymal stem cells (MSCs) in a cynomolgus monkey model of islet/bone marrow transplantation.
doi10.2337/db10-0136
pmid20622174
issn00121797
oafree_for_read
date2010-10