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Asymmetric biocatalytic reduction of 3,5-bis(trifluoromethyl) acetophenone to (1R)-3,5-bis(trifluoromethyl)phenyl ethanol using whole cells of newly isolated Leifsonia xyli HS0904

A novel bacterial strain HS0904 was isolated from a soil sample using 3,5-bis(trifluoromethyl) acetophenone as the sole carbon source. This bacterial isolate can asymmetrically reduce 3,5-bis(trifluoromethyl) acetophenone to (1R)-[3,5-bis(trifluoromethyl)phenyl] ethanol with high enantiometric exces... Full description

Journal Title: Applied microbiology and biotechnology June 2011, Vol.90(6), pp.1897-904
Main Author: Wang, Pu
Other Authors: Cai, Jin-Bo , Ouyang, Qi , He, Jun-Yao , Su, Hui-Zhen
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: E-ISSN: 1432-0614 ; PMID: 21614678 Version:1 ; DOI: 10.1007/s00253-011-3233-3
Link: http://pubmed.gov/21614678
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recordid: medline21614678
title: Asymmetric biocatalytic reduction of 3,5-bis(trifluoromethyl) acetophenone to (1R)-3,5-bis(trifluoromethyl)phenyl ethanol using whole cells of newly isolated Leifsonia xyli HS0904
format: Article
creator:
  • Wang, Pu
  • Cai, Jin-Bo
  • Ouyang, Qi
  • He, Jun-Yao
  • Su, Hui-Zhen
subjects:
  • Soil Microbiology
  • Acetophenones -- Metabolism
  • Actinomycetales -- Classification
  • Benzyl Alcohols -- Metabolism
ispartof: Applied microbiology and biotechnology, June 2011, Vol.90(6), pp.1897-904
description: A novel bacterial strain HS0904 was isolated from a soil sample using 3,5-bis(trifluoromethyl) acetophenone as the sole carbon source. This bacterial isolate can asymmetrically reduce 3,5-bis(trifluoromethyl) acetophenone to (1R)-[3,5-bis(trifluoromethyl)phenyl] ethanol with high enantiometric excess (ee) value. Based on its morphological, physiological characteristics, Biolog, 16S rDNA sequence and phylogenetic analysis, strain HS0904 was identified as Leifsonia xyli HS0904. To our knowledge, this is the first reported case on the species L. xyli exhibited R-stereospecific carbonyl reductase and used for the preparation of chiral (1R)-[3,5-bis(trifluoromethyl)phenyl] ethanol. The optimization of parameters for microbial transformation of 3,5-bis(trifluoromethyl) acetophenone to (1R)-[3,5-bis(trifluoromethyl)phenyl] ethanol catalyzed by whole cells of L. xyli HS0904 was carried out by examining some key factors including buffer pH, reaction temperature, shaking speed, substrate concentration,...
language: eng
source:
identifier: E-ISSN: 1432-0614 ; PMID: 21614678 Version:1 ; DOI: 10.1007/s00253-011-3233-3
fulltext: fulltext
issn:
  • 14320614
  • 1432-0614
url: Link


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titleAsymmetric biocatalytic reduction of 3,5-bis(trifluoromethyl) acetophenone to (1R)-3,5-bis(trifluoromethyl)phenyl ethanol using whole cells of newly isolated Leifsonia xyli HS0904
creatorWang, Pu ; Cai, Jin-Bo ; Ouyang, Qi ; He, Jun-Yao ; Su, Hui-Zhen
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subjectSoil Microbiology ; Acetophenones -- Metabolism ; Actinomycetales -- Classification ; Benzyl Alcohols -- Metabolism
descriptionA novel bacterial strain HS0904 was isolated from a soil sample using 3,5-bis(trifluoromethyl) acetophenone as the sole carbon source. This bacterial isolate can asymmetrically reduce 3,5-bis(trifluoromethyl) acetophenone to (1R)-[3,5-bis(trifluoromethyl)phenyl] ethanol with high enantiometric excess (ee) value. Based on its morphological, physiological characteristics, Biolog, 16S rDNA sequence and phylogenetic analysis, strain HS0904 was identified as Leifsonia xyli HS0904. To our knowledge, this is the first reported case on the species L. xyli exhibited R-stereospecific carbonyl reductase and used for the preparation of chiral (1R)-[3,5-bis(trifluoromethyl)phenyl] ethanol. The optimization of parameters for microbial transformation of 3,5-bis(trifluoromethyl) acetophenone to (1R)-[3,5-bis(trifluoromethyl)phenyl] ethanol catalyzed by whole cells of L. xyli HS0904 was carried out by examining some key factors including buffer pH, reaction temperature, shaking speed, substrate concentration,...
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descriptionA novel bacterial strain HS0904 was isolated from a soil sample using 3,5-bis(trifluoromethyl) acetophenone as the sole carbon source. This bacterial isolate can asymmetrically reduce 3,5-bis(trifluoromethyl) acetophenone to (1R)-[3,5-bis(trifluoromethyl)phenyl] ethanol with high enantiometric excess (ee) value. Based on its morphological, physiological characteristics, Biolog, 16S rDNA sequence and phylogenetic analysis, strain HS0904 was identified as Leifsonia xyli HS0904. To our knowledge, this is the first reported case on the species L. xyli exhibited R-stereospecific carbonyl reductase and used for the preparation of chiral (1R)-[3,5-bis(trifluoromethyl)phenyl] ethanol. The optimization of parameters for microbial transformation of 3,5-bis(trifluoromethyl) acetophenone to (1R)-[3,5-bis(trifluoromethyl)phenyl] ethanol catalyzed by whole cells of L. xyli HS0904 was carried out by examining some key factors including buffer pH, reaction temperature, shaking speed, substrate concentration,...
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abstractA novel bacterial strain HS0904 was isolated from a soil sample using 3,5-bis(trifluoromethyl) acetophenone as the sole carbon source. This bacterial isolate can asymmetrically reduce 3,5-bis(trifluoromethyl) acetophenone to (1R)-[3,5-bis(trifluoromethyl)phenyl] ethanol with high enantiometric excess (ee) value. Based on its morphological, physiological characteristics, Biolog, 16S rDNA sequence and phylogenetic analysis, strain HS0904 was identified as Leifsonia xyli HS0904. To our knowledge, this is the first reported case on the species L. xyli exhibited R-stereospecific carbonyl reductase and used for the preparation of chiral (1R)-[3,5-bis(trifluoromethyl)phenyl] ethanol. The optimization of parameters for microbial transformation of 3,5-bis(trifluoromethyl) acetophenone to (1R)-[3,5-bis(trifluoromethyl)phenyl] ethanol catalyzed by whole cells of L. xyli HS0904 was carried out by examining some key factors including buffer pH, reaction temperature, shaking speed, substrate concentration,...
doi10.1007/s00253-011-3233-3
pmid21614678
date2011-06