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DNA sensing by amplifying the number of near-infrared emitting, oligonucleotide-encapsulated silver clusters

A bifunctional oligonucleotide integrates in situ synthesis of a fluorogenic silver cluster with recognition of a target DNA sequence. With the template C(3)AC(3)AC(3)GC(3)A, a complex forms with 10 silver atoms that possesses electronic transitions in the near-infrared and that is detected at nanom... Full description

Journal Title: Analytical chemistry 01 August 2011, Vol.83(15), pp.5957-64
Main Author: Petty, Jeffrey T
Other Authors: Sengupta, Bidisha , Story, Sandra P , Degtyareva, Natalya N
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: E-ISSN: 1520-6882 ; PMID: 21702495 Version:1 ; DOI: 10.1021/ac201321m
Link: http://pubmed.gov/21702495
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recordid: medline21702495
title: DNA sensing by amplifying the number of near-infrared emitting, oligonucleotide-encapsulated silver clusters
format: Article
creator:
  • Petty, Jeffrey T
  • Sengupta, Bidisha
  • Story, Sandra P
  • Degtyareva, Natalya N
subjects:
  • DNA -- Analysis
  • Nucleic Acid Hybridization -- Methods
  • Oligonucleotides -- Chemistry
  • Silver -- Chemistry
  • Spectroscopy, Near-Infrared -- Methods
ispartof: Analytical chemistry, 01 August 2011, Vol.83(15), pp.5957-64
description: A bifunctional oligonucleotide integrates in situ synthesis of a fluorogenic silver cluster with recognition of a target DNA sequence. With the template C(3)AC(3)AC(3)GC(3)A, a complex forms with 10 silver atoms that possesses electronic transitions in the near-infrared and that is detected at nanomolar concentrations using diode laser excitation. Pendant to this cluster encoding region, the recognition component binds a target DNA strand through hybridization, and decoupling of these two regions of the composite sensor renders a modular sensor for specific oligonucleotides. A target is detected using a quencher strand that bridges the cluster template and recognition components and disturbs cluster binding, as indicated by static quenching. Competitive displacement of the quencher by the target strand restores the favored cluster environment, and our key finding is that this exchange enhances emission through a proportional increase in the number of emissive clusters. DNA detection is also accomplished in serum-containing buffers by taking advantage of the high brightness of this fluorophore and the inherently low endogenous background in the near-infrared spectral region. Cluster stability in this biological environment is enhanced by supplementing the solutions with Ag(+).
language: eng
source:
identifier: E-ISSN: 1520-6882 ; PMID: 21702495 Version:1 ; DOI: 10.1021/ac201321m
fulltext: no_fulltext
issn:
  • 15206882
  • 1520-6882
url: Link


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titleDNA sensing by amplifying the number of near-infrared emitting, oligonucleotide-encapsulated silver clusters
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ispartofAnalytical chemistry, 01 August 2011, Vol.83(15), pp.5957-64
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subjectDNA -- Analysis ; Nucleic Acid Hybridization -- Methods ; Oligonucleotides -- Chemistry ; Silver -- Chemistry ; Spectroscopy, Near-Infrared -- Methods
descriptionA bifunctional oligonucleotide integrates in situ synthesis of a fluorogenic silver cluster with recognition of a target DNA sequence. With the template C(3)AC(3)AC(3)GC(3)A, a complex forms with 10 silver atoms that possesses electronic transitions in the near-infrared and that is detected at nanomolar concentrations using diode laser excitation. Pendant to this cluster encoding region, the recognition component binds a target DNA strand through hybridization, and decoupling of these two regions of the composite sensor renders a modular sensor for specific oligonucleotides. A target is detected using a quencher strand that bridges the cluster template and recognition components and disturbs cluster binding, as indicated by static quenching. Competitive displacement of the quencher by the target strand restores the favored cluster environment, and our key finding is that this exchange enhances emission through a proportional increase in the number of emissive clusters. DNA detection is also accomplished in serum-containing buffers by taking advantage of the high brightness of this fluorophore and the inherently low endogenous background in the near-infrared spectral region. Cluster stability in this biological environment is enhanced by supplementing the solutions with Ag(+).
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abstractA bifunctional oligonucleotide integrates in situ synthesis of a fluorogenic silver cluster with recognition of a target DNA sequence. With the template C(3)AC(3)AC(3)GC(3)A, a complex forms with 10 silver atoms that possesses electronic transitions in the near-infrared and that is detected at nanomolar concentrations using diode laser excitation. Pendant to this cluster encoding region, the recognition component binds a target DNA strand through hybridization, and decoupling of these two regions of the composite sensor renders a modular sensor for specific oligonucleotides. A target is detected using a quencher strand that bridges the cluster template and recognition components and disturbs cluster binding, as indicated by static quenching. Competitive displacement of the quencher by the target strand restores the favored cluster environment, and our key finding is that this exchange enhances emission through a proportional increase in the number of emissive clusters. DNA detection is also accomplished in serum-containing buffers by taking advantage of the high brightness of this fluorophore and the inherently low endogenous background in the near-infrared spectral region. Cluster stability in this biological environment is enhanced by supplementing the solutions with Ag(+).
doi10.1021/ac201321m
pmid21702495
issn00032700
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date2011-08-01