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In vivo serial MR imaging of magnetically labeled endothelial progenitor cells homing to the endothelium injured artery in mice

Emerging evidence of histopathological analyses suggests that endothelial progenitor cells (EPCs) play an important role in vascular diseases. Neointimal hyperplasia can be reduced by intravenous transfusion of EPCs after vascular injury in mice. Therefore, it would be advantageous to develop an in... Full description

Journal Title: PloS one 2011, Vol.6(6), pp.e20790
Main Author: Chen, Jun
Other Authors: Jia, Zhen-Yu , Ma, Zhan-Long , Wang, Yuan-Yuan , Teng, Gao-Jun
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: E-ISSN: 1932-6203 ; PMID: 21731624 Version:1 ; DOI: 10.1371/journal.pone.0020790
Link: http://pubmed.gov/21731624
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title: In vivo serial MR imaging of magnetically labeled endothelial progenitor cells homing to the endothelium injured artery in mice
format: Article
creator:
  • Chen, Jun
  • Jia, Zhen-Yu
  • Ma, Zhan-Long
  • Wang, Yuan-Yuan
  • Teng, Gao-Jun
subjects:
  • Magnetics
  • Staining and Labeling
  • Arteries -- Pathology
  • Endothelial Cells -- Cytology
  • Endothelium, Vascular -- Pathology
  • Magnetic Resonance Imaging -- Methods
  • Stem Cells -- Cytology
ispartof: PloS one, 2011, Vol.6(6), pp.e20790
description: Emerging evidence of histopathological analyses suggests that endothelial progenitor cells (EPCs) play an important role in vascular diseases. Neointimal hyperplasia can be reduced by intravenous transfusion of EPCs after vascular injury in mice. Therefore, it would be advantageous to develop an in vivo technique that can explore the temporal and spatial migration of EPCs homing to the damaged endothelium noninvasively. The left carotid common artery (LCCA) was injured by removal of endothelium with a flexible wire in Kunming mice. EPCs were collected by in vitro culture of spleen-derived mouse mononuclear cells (MNCs). EPCs labeling was carried out in vitro using Fe₂O₃-poly-L-lysine (Fe₂O₃-PLL). In vivo serial MR imaging was performed to follow-up the injured artery at different time points after intravenous transfusion of EPCs. Vessel wall areas of injured artery were computed on T₂WI. Larger MR signal voids of vessel wall on T₂WI was revealed in all 6 mice of the labeled EPC transfusion group 15 days after LCCA injury, and it was found only in 1 mouse in the unlabeled EPC transfusion group (p = 0.015). Quantitative analyses of vessel wall areas on T₂WI showed that the vessel wall areas of labeled EPC transfusion group were less than those of unlabeled EPC transfusion group and control group fifteen days after artery injury (p
language: eng
source:
identifier: E-ISSN: 1932-6203 ; PMID: 21731624 Version:1 ; DOI: 10.1371/journal.pone.0020790
fulltext: fulltext
issn:
  • 19326203
  • 1932-6203
url: Link


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titleIn vivo serial MR imaging of magnetically labeled endothelial progenitor cells homing to the endothelium injured artery in mice
creatorChen, Jun ; Jia, Zhen-Yu ; Ma, Zhan-Long ; Wang, Yuan-Yuan ; Teng, Gao-Jun
ispartofPloS one, 2011, Vol.6(6), pp.e20790
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subjectMagnetics ; Staining and Labeling ; Arteries -- Pathology ; Endothelial Cells -- Cytology ; Endothelium, Vascular -- Pathology ; Magnetic Resonance Imaging -- Methods ; Stem Cells -- Cytology
descriptionEmerging evidence of histopathological analyses suggests that endothelial progenitor cells (EPCs) play an important role in vascular diseases. Neointimal hyperplasia can be reduced by intravenous transfusion of EPCs after vascular injury in mice. Therefore, it would be advantageous to develop an in vivo technique that can explore the temporal and spatial migration of EPCs homing to the damaged endothelium noninvasively. The left carotid common artery (LCCA) was injured by removal of endothelium with a flexible wire in Kunming mice. EPCs were collected by in vitro culture of spleen-derived mouse mononuclear cells (MNCs). EPCs labeling was carried out in vitro using Fe₂O₃-poly-L-lysine (Fe₂O₃-PLL). In vivo serial MR imaging was performed to follow-up the injured artery at different time points after intravenous transfusion of EPCs. Vessel wall areas of injured artery were computed on T₂WI. Larger MR signal voids of vessel wall on T₂WI was revealed in all 6 mice of the labeled EPC transfusion group 15 days after LCCA injury, and it was found only in 1 mouse in the unlabeled EPC transfusion group (p = 0.015). Quantitative analyses of vessel wall areas on T₂WI showed that the vessel wall areas of labeled EPC transfusion group were less than those of unlabeled EPC transfusion group and control group fifteen days after artery injury (p<0.05). Histopathological analyses confirmed accumulation and distribution... These data indicate that MR imaging might be used as an in vivo method for the tracking of EPCs homing to the endothelium injured artery.
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abstractEmerging evidence of histopathological analyses suggests that endothelial progenitor cells (EPCs) play an important role in vascular diseases. Neointimal hyperplasia can be reduced by intravenous transfusion of EPCs after vascular injury in mice. Therefore, it would be advantageous to develop an in vivo technique that can explore the temporal and spatial migration of EPCs homing to the damaged endothelium noninvasively.
doi10.1371/journal.pone.0020790
pmid21731624