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Use of fluorescent DNA-templated gold/silver nanoclusters for the detection of sulfide ions

We have developed a one-pot approach to prepare fluorescent DNA-templated gold/silver nanoclusters (DNA-Au/Ag NCs) from Au(3+), Ag(+), and DNA (5'-CCCTTAATCCCC-3') in the presence of NaBH(4) in order to detect sulfide (S(2-)) ions on the basis of fluorescence quenching. The as-prepared DNA-Au/Ag NCs... Full description

Journal Title: Analytical chemistry 15 December 2011, Vol.83(24), pp.9450-5
Main Author: Chen, Wei-Yu
Other Authors: Lan, Guo-Yu , Chang, Huan-Tsung
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: E-ISSN: 1520-6882 ; PMID: 22029551 Version:1 ; DOI: 10.1021/ac202162u
Link: http://pubmed.gov/22029551
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title: Use of fluorescent DNA-templated gold/silver nanoclusters for the detection of sulfide ions
format: Article
creator:
  • Chen, Wei-Yu
  • Lan, Guo-Yu
  • Chang, Huan-Tsung
subjects:
  • Spectrophotometry, Ultraviolet
  • DNA -- Chemistry
  • Gold -- Chemistry
  • Metal Nanoparticles -- Chemistry
  • Silver -- Chemistry
  • Sulfides -- Analysis
ispartof: Analytical chemistry, 15 December 2011, Vol.83(24), pp.9450-5
description: We have developed a one-pot approach to prepare fluorescent DNA-templated gold/silver nanoclusters (DNA-Au/Ag NCs) from Au(3+), Ag(+), and DNA (5'-CCCTTAATCCCC-3') in the presence of NaBH(4) in order to detect sulfide (S(2-)) ions on the basis of fluorescence quenching. The as-prepared DNA-Au/Ag NCs have been characterized by UV-vis absorption, fluorescence, circular dichroism, X-ray photoelectron spectroscopy, and electrospray ionization-mass spectrometry measurements. Relative to DNA-Ag NCs, DNA-Au/Ag NCs are much more stable in high ionic strength media (e.g., 200 mM NaCl). The quantum yield of the as-prepared DNA-Au/Ag NCs is 4.5%. We have demonstrated that the fluorescence of DNA-Au/Ag NCs is quenched by S(2-) ions through the interaction between sulfide ions and gold/silver atoms/ions, a result which leads to changes in the conformation of the templated DNA from packed hairpin to random coil structures. These changes in fluorescence intensity allow sensitive detection of S(2-) ions at concentrations as low as 0.83 nM. To minimize interference from I(-) ions for the detection of S(2-) ions using the DNA-Au/Ag NCs, the addition of sodium peroxydisulfate to the solution is essential. We have validated the practicality of this probe for the detection of S(2-) ions in hot spring and seawater samples, demonstrating its advantages of simplicity, sensitivity, selectivity, and low cost.
language: eng
source:
identifier: E-ISSN: 1520-6882 ; PMID: 22029551 Version:1 ; DOI: 10.1021/ac202162u
fulltext: fulltext
issn:
  • 15206882
  • 1520-6882
url: Link


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titleUse of fluorescent DNA-templated gold/silver nanoclusters for the detection of sulfide ions
creatorChen, Wei-Yu ; Lan, Guo-Yu ; Chang, Huan-Tsung
ispartofAnalytical chemistry, 15 December 2011, Vol.83(24), pp.9450-5
identifier
subjectSpectrophotometry, Ultraviolet ; DNA -- Chemistry ; Gold -- Chemistry ; Metal Nanoparticles -- Chemistry ; Silver -- Chemistry ; Sulfides -- Analysis
descriptionWe have developed a one-pot approach to prepare fluorescent DNA-templated gold/silver nanoclusters (DNA-Au/Ag NCs) from Au(3+), Ag(+), and DNA (5'-CCCTTAATCCCC-3') in the presence of NaBH(4) in order to detect sulfide (S(2-)) ions on the basis of fluorescence quenching. The as-prepared DNA-Au/Ag NCs have been characterized by UV-vis absorption, fluorescence, circular dichroism, X-ray photoelectron spectroscopy, and electrospray ionization-mass spectrometry measurements. Relative to DNA-Ag NCs, DNA-Au/Ag NCs are much more stable in high ionic strength media (e.g., 200 mM NaCl). The quantum yield of the as-prepared DNA-Au/Ag NCs is 4.5%. We have demonstrated that the fluorescence of DNA-Au/Ag NCs is quenched by S(2-) ions through the interaction between sulfide ions and gold/silver atoms/ions, a result which leads to changes in the conformation of the templated DNA from packed hairpin to random coil structures. These changes in fluorescence intensity allow sensitive detection of S(2-) ions at concentrations as low as 0.83 nM. To minimize interference from I(-) ions for the detection of S(2-) ions using the DNA-Au/Ag NCs, the addition of sodium peroxydisulfate to the solution is essential. We have validated the practicality of this probe for the detection of S(2-) ions in hot spring and seawater samples, demonstrating its advantages of simplicity, sensitivity, selectivity, and low cost.
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descriptionWe have developed a one-pot approach to prepare fluorescent DNA-templated gold/silver nanoclusters (DNA-Au/Ag NCs) from Au(3+), Ag(+), and DNA (5'-CCCTTAATCCCC-3') in the presence of NaBH(4) in order to detect sulfide (S(2-)) ions on the basis of fluorescence quenching. The as-prepared DNA-Au/Ag NCs have been characterized by UV-vis absorption, fluorescence, circular dichroism, X-ray photoelectron spectroscopy, and electrospray ionization-mass spectrometry measurements. Relative to DNA-Ag NCs, DNA-Au/Ag NCs are much more stable in high ionic strength media (e.g., 200 mM NaCl). The quantum yield of the as-prepared DNA-Au/Ag NCs is 4.5%. We have demonstrated that the fluorescence of DNA-Au/Ag NCs is quenched by S(2-) ions through the interaction between sulfide ions and gold/silver atoms/ions, a result which leads to changes in the conformation of the templated DNA from packed hairpin to random coil structures. These changes in fluorescence intensity allow sensitive detection of S(2-) ions at concentrations as low as 0.83 nM. To minimize interference from I(-) ions for the detection of S(2-) ions using the DNA-Au/Ag NCs, the addition of sodium peroxydisulfate to the solution is essential. We have validated the practicality of this probe for the detection of S(2-) ions in hot spring and seawater samples, demonstrating its advantages of simplicity, sensitivity, selectivity, and low cost.
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abstractWe have developed a one-pot approach to prepare fluorescent DNA-templated gold/silver nanoclusters (DNA-Au/Ag NCs) from Au(3+), Ag(+), and DNA (5'-CCCTTAATCCCC-3') in the presence of NaBH(4) in order to detect sulfide (S(2-)) ions on the basis of fluorescence quenching. The as-prepared DNA-Au/Ag NCs have been characterized by UV-vis absorption, fluorescence, circular dichroism, X-ray photoelectron spectroscopy, and electrospray ionization-mass spectrometry measurements. Relative to DNA-Ag NCs, DNA-Au/Ag NCs are much more stable in high ionic strength media (e.g., 200 mM NaCl). The quantum yield of the as-prepared DNA-Au/Ag NCs is 4.5%. We have demonstrated that the fluorescence of DNA-Au/Ag NCs is quenched by S(2-) ions through the interaction between sulfide ions and gold/silver atoms/ions, a result which leads to changes in the conformation of the templated DNA from packed hairpin to random coil structures. These changes in fluorescence intensity allow sensitive detection of S(2-) ions at concentrations as low as 0.83 nM. To minimize interference from I(-) ions for the detection of S(2-) ions using the DNA-Au/Ag NCs, the addition of sodium peroxydisulfate to the solution is essential. We have validated the practicality of this probe for the detection of S(2-) ions in hot spring and seawater samples, demonstrating its advantages of simplicity, sensitivity, selectivity, and low cost.
doi10.1021/ac202162u
pmid22029551
issn00032700
oafree_for_read
date2011-12-15