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Interaction between microRNAs and actin-associated protein Arpc5 regulates translational suppression during male germ cell differentiation

Decoupling of transcription and translation during postmeiotic germ cell differentiation is critical for successful spermatogenesis. Here we establish that the interaction between microRNAs and actin-associated protein Arpc5 sets the stage for an elaborate translational control mechanism by facilita... Full description

Journal Title: Proceedings of the National Academy of Sciences of the United States of America 10 April 2012, Vol.109(15), pp.5750-5
Main Author: Chang, Yao-Fu
Other Authors: Lee-Chang, Jennifer S , Imam, J Saadi , Buddavarapu, Kalyan Chakravarthy , Subaran, Sarah S , Sinha-Hikim, Amiya P , Gorospe, Myriam , Rao, Manjeet K
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: E-ISSN: 1091-6490 ; PMID: 22447776 Version:1 ; DOI: 10.1073/pnas.1117837109
Link: http://pubmed.gov/22447776
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recordid: medline22447776
title: Interaction between microRNAs and actin-associated protein Arpc5 regulates translational suppression during male germ cell differentiation
format: Article
creator:
  • Chang, Yao-Fu
  • Lee-Chang, Jennifer S
  • Imam, J Saadi
  • Buddavarapu, Kalyan Chakravarthy
  • Subaran, Sarah S
  • Sinha-Hikim, Amiya P
  • Gorospe, Myriam
  • Rao, Manjeet K
subjects:
  • Actin-Related Protein 2-3 Complex -- Metabolism
  • Cell Differentiation -- Genetics
  • Micrornas -- Metabolism
  • Spermatozoa -- Metabolism
ispartof: Proceedings of the National Academy of Sciences of the United States of America, 10 April 2012, Vol.109(15), pp.5750-5
description: Decoupling of transcription and translation during postmeiotic germ cell differentiation is critical for successful spermatogenesis. Here we establish that the interaction between microRNAs and actin-associated protein Arpc5 sets the stage for an elaborate translational control mechanism by facilitating the sequestration of germ cell mRNAs into translationally inert ribonucleoprotein particles until they are later translated. Our studies reveal that loss of microRNA-dependent regulation of Arpc5, which controls the distribution of germ cell mRNAs between translationally active and inactive pools, results in abnormal round spermatid differentiation and impaired fertility. Interestingly, Arpc5 functions as a broadly acting translational suppressor, as it inhibits translation initiation by blocking 80S formation and facilitates the transport of mRNAs to chromatoid/P bodies. These findings identify a unique role for actin-associated proteins in translational regulation, and suggest that mRNA-specific...
language: eng
source:
identifier: E-ISSN: 1091-6490 ; PMID: 22447776 Version:1 ; DOI: 10.1073/pnas.1117837109
fulltext: fulltext
issn:
  • 10916490
  • 1091-6490
url: Link


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titleInteraction between microRNAs and actin-associated protein Arpc5 regulates translational suppression during male germ cell differentiation
creatorChang, Yao-Fu ; Lee-Chang, Jennifer S ; Imam, J Saadi ; Buddavarapu, Kalyan Chakravarthy ; Subaran, Sarah S ; Sinha-Hikim, Amiya P ; Gorospe, Myriam ; Rao, Manjeet K
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subjectActin-Related Protein 2-3 Complex -- Metabolism ; Cell Differentiation -- Genetics ; Micrornas -- Metabolism ; Spermatozoa -- Metabolism
descriptionDecoupling of transcription and translation during postmeiotic germ cell differentiation is critical for successful spermatogenesis. Here we establish that the interaction between microRNAs and actin-associated protein Arpc5 sets the stage for an elaborate translational control mechanism by facilitating the sequestration of germ cell mRNAs into translationally inert ribonucleoprotein particles until they are later translated. Our studies reveal that loss of microRNA-dependent regulation of Arpc5, which controls the distribution of germ cell mRNAs between translationally active and inactive pools, results in abnormal round spermatid differentiation and impaired fertility. Interestingly, Arpc5 functions as a broadly acting translational suppressor, as it inhibits translation initiation by blocking 80S formation and facilitates the transport of mRNAs to chromatoid/P bodies. These findings identify a unique role for actin-associated proteins in translational regulation, and suggest that mRNA-specific...
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titleInteraction between microRNAs and actin-associated protein Arpc5 regulates translational suppression during male germ cell differentiation
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titleInteraction between microRNAs and actin-associated protein Arpc5 regulates translational suppression during male germ cell differentiation
authorChang, Yao-Fu ; Lee-Chang, Jennifer S ; Imam, J Saadi ; Buddavarapu, Kalyan Chakravarthy ; Subaran, Sarah S ; Sinha-Hikim, Amiya P ; Gorospe, Myriam ; Rao, Manjeet K
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abstractDecoupling of transcription and translation during postmeiotic germ cell differentiation is critical for successful spermatogenesis. Here we establish that the interaction between microRNAs and actin-associated protein Arpc5 sets the stage for an elaborate translational control mechanism by facilitating the sequestration of germ cell mRNAs into translationally inert ribonucleoprotein particles until they are later translated. Our studies reveal that loss of microRNA-dependent regulation of Arpc5, which controls the distribution of germ cell mRNAs between translationally active and inactive pools, results in abnormal round spermatid differentiation and impaired fertility. Interestingly, Arpc5 functions as a broadly acting translational suppressor, as it inhibits translation initiation by blocking 80S formation and facilitates the transport of mRNAs to chromatoid/P bodies. These findings identify a unique role for actin-associated proteins in translational regulation, and suggest that mRNA-specific...
doi10.1073/pnas.1117837109
pmid22447776
date2012-04-10