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Increased importin 13 activity is associated with the pathogenesis of pterygium

We previously reported that importin 13 (IPO13), a member of the importin-β family of nuclear import proteins, regulates nuclear import of the glucocorticoid receptor in airway epithelial cells, IPO13 serves as a potential marker for corneal epithelial progenitor cells, and IPO13 is associated with... Full description

Journal Title: Molecular vision 2013, Vol.19, pp.604-13
Main Author: Xu, Ke
Other Authors: Tao, Tao , Jie, Jing , Lu, Xiaodong , Li, Xuezhi , Mehmood, Muhammad Aamer , He, Hui , Liu, Zhen , Xiao, Xinye , Yang, Jie , Ma, Jian-Xing , Li, Wei , Zhou, Yueping , Liu, Zuguo
Format: Electronic Article Electronic Article
Language: English
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Quelle: MEDLINE/PubMed (U.S. National Library of Medicine)
ID: E-ISSN: 1090-0535 ; PMID: 23559854 Version:1
Link: http://pubmed.gov/23559854
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title: Increased importin 13 activity is associated with the pathogenesis of pterygium
format: Article
creator:
  • Xu, Ke
  • Tao, Tao
  • Jie, Jing
  • Lu, Xiaodong
  • Li, Xuezhi
  • Mehmood, Muhammad Aamer
  • He, Hui
  • Liu, Zhen
  • Xiao, Xinye
  • Yang, Jie
  • Ma, Jian-Xing
  • Li, Wei
  • Zhou, Yueping
  • Liu, Zuguo
subjects:
  • Karyopherins -- Metabolism
  • Pterygium -- Etiology
ispartof: Molecular vision, 2013, Vol.19, pp.604-13
description: We previously reported that importin 13 (IPO13), a member of the importin-β family of nuclear import proteins, regulates nuclear import of the glucocorticoid receptor in airway epithelial cells, IPO13 serves as a potential marker for corneal epithelial progenitor cells, and IPO13 is associated with corneal cell proliferation. Here we investigated the role of IPO13 in the pathogenesis of pterygium and the underlying mechanism including interaction with other cell proliferation-related factors: keratin 17 (K17), a lesional protein and a member of the type I keratins, and c-Jun, a protein of the activator protein-1 complex. Tissue samples were collected from primary pterygia, recurrent pterygia, and normal conjunctiva to perform the following experiments: immunohistochemical measurement of IPO13 and K17. Pterygium epithelial cells (PECs) were cultured in keratinocyte serum-free defined medium to examine the expression of IPO13 and K17. Lentivirus-mediated silencing and overexpression IPO13 testing was conducted, and K17 alternation was evaluated with western blot and immunostaining. In addition, the translocation of c-Jun (a K17 regulator) was further examined after IPO13 was silenced. IPO13 activity was significantly increased in the basal layer of the epithelium of the pterygium. In cultured PECs, overexpression or knockdown of the IPO13 gene increased or decreased PEC proliferation, respectively. IPO13 was colocalized with K17 in the epithelium of the pterygium, and overexpression or knockdown of the IPO13 gene induced upregulation or downregulation of K17 expression in PECs, respectively. In addition, silencing of the IPO13 gene blocked nuclear translocation of c-Jun. We provided novel evidence that IPO13 may contribute to the pathogenesis of pterygium via modulation of K17 and c-Jun.
language: eng
source: MEDLINE/PubMed (U.S. National Library of Medicine)
identifier: E-ISSN: 1090-0535 ; PMID: 23559854 Version:1
fulltext: fulltext
issn:
  • 10900535
  • 1090-0535
url: Link


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titleIncreased importin 13 activity is associated with the pathogenesis of pterygium
creatorXu, Ke ; Tao, Tao ; Jie, Jing ; Lu, Xiaodong ; Li, Xuezhi ; Mehmood, Muhammad Aamer ; He, Hui ; Liu, Zhen ; Xiao, Xinye ; Yang, Jie ; Ma, Jian-Xing ; Li, Wei ; Zhou, Yueping ; Liu, Zuguo
ispartofMolecular vision, 2013, Vol.19, pp.604-13
identifierE-ISSN: 1090-0535 ; PMID: 23559854 Version:1
subjectKaryopherins -- Metabolism ; Pterygium -- Etiology
descriptionWe previously reported that importin 13 (IPO13), a member of the importin-β family of nuclear import proteins, regulates nuclear import of the glucocorticoid receptor in airway epithelial cells, IPO13 serves as a potential marker for corneal epithelial progenitor cells, and IPO13 is associated with corneal cell proliferation. Here we investigated the role of IPO13 in the pathogenesis of pterygium and the underlying mechanism including interaction with other cell proliferation-related factors: keratin 17 (K17), a lesional protein and a member of the type I keratins, and c-Jun, a protein of the activator protein-1 complex. Tissue samples were collected from primary pterygia, recurrent pterygia, and normal conjunctiva to perform the following experiments: immunohistochemical measurement of IPO13 and K17. Pterygium epithelial cells (PECs) were cultured in keratinocyte serum-free defined medium to examine the expression of IPO13 and K17. Lentivirus-mediated silencing and overexpression IPO13 testing was conducted, and K17 alternation was evaluated with western blot and immunostaining. In addition, the translocation of c-Jun (a K17 regulator) was further examined after IPO13 was silenced. IPO13 activity was significantly increased in the basal layer of the epithelium of the pterygium. In cultured PECs, overexpression or knockdown of the IPO13 gene increased or decreased PEC proliferation, respectively. IPO13 was colocalized with K17 in the epithelium of the pterygium, and overexpression or knockdown of the IPO13 gene induced upregulation or downregulation of K17 expression in PECs, respectively. In addition, silencing of the IPO13 gene blocked nuclear translocation of c-Jun. We provided novel evidence that IPO13 may contribute to the pathogenesis of pterygium via modulation of K17 and c-Jun.
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2IPO13 activity was significantly increased in the basal layer of the epithelium of the pterygium. In cultured PECs, overexpression or knockdown of the IPO13 gene increased or decreased PEC proliferation, respectively. IPO13 was colocalized with K17 in the epithelium of the pterygium, and overexpression or knockdown of the IPO13 gene induced upregulation or downregulation of K17 expression in PECs, respectively. In addition, silencing of the IPO13 gene blocked nuclear translocation of c-Jun.
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abstractWe previously reported that importin 13 (IPO13), a member of the importin-β family of nuclear import proteins, regulates nuclear import of the glucocorticoid receptor in airway epithelial cells, IPO13 serves as a potential marker for corneal epithelial progenitor cells, and IPO13 is associated with corneal cell proliferation. Here we investigated the role of IPO13 in the pathogenesis of pterygium and the underlying mechanism including interaction with other cell proliferation-related factors: keratin 17 (K17), a lesional protein and a member of the type I keratins, and c-Jun, a protein of the activator protein-1 complex.
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