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Good thermally conducting material supports follicle morphologies of porcine ovaries cryopreserved with ultrarapid vitrification

Effects of supporting materials during vitrification procedure on the morphologies of preantral follicles of pig ovaries were assessed. Ovarian cortical sections of prepubertal pigs were randomly allocated to 5 groups. The sections were vitrified ultrarapidly with 5 different vitrification devices.... Full description

Journal Title: The Journal of reproduction and development October 2013, Vol.59(5), pp.496-9
Main Author: Hashimoto, Shu
Other Authors: Suzuki, Nao , Amo, Ami , Yamochi, Takayuki , Hosoi, Yoshihiko , Morimoto, Yoshiharu
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: E-ISSN: 1348-4400 ; PMID: 23774861 Version:1
Link: http://pubmed.gov/23774861
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recordid: medline23774861
title: Good thermally conducting material supports follicle morphologies of porcine ovaries cryopreserved with ultrarapid vitrification
format: Article
creator:
  • Hashimoto, Shu
  • Suzuki, Nao
  • Amo, Ami
  • Yamochi, Takayuki
  • Hosoi, Yoshihiko
  • Morimoto, Yoshiharu
subjects:
  • Sus Scrofa
  • Vitrification
  • Cryopreservation -- Instrumentation
  • Ovary -- Cytology
ispartof: The Journal of reproduction and development, October 2013, Vol.59(5), pp.496-9
description: Effects of supporting materials during vitrification procedure on the morphologies of preantral follicles of pig ovaries were assessed. Ovarian cortical sections of prepubertal pigs were randomly allocated to 5 groups. The sections were vitrified ultrarapidly with 5 different vitrification devices. The sections were put on 4 fine needles (Cryosupport), on a thin copper plate, or on a carbon graphite sheet or were sandwiched between copper plates or between carbon graphite sheets before cooling. The cooling and warming rates with the graphite sheets were significantly higher than those with the copper plates (P
language: eng
source:
identifier: E-ISSN: 1348-4400 ; PMID: 23774861 Version:1
fulltext: fulltext
issn:
  • 13484400
  • 1348-4400
url: Link


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titleGood thermally conducting material supports follicle morphologies of porcine ovaries cryopreserved with ultrarapid vitrification
creatorHashimoto, Shu ; Suzuki, Nao ; Amo, Ami ; Yamochi, Takayuki ; Hosoi, Yoshihiko ; Morimoto, Yoshiharu
ispartofThe Journal of reproduction and development, October 2013, Vol.59(5), pp.496-9
identifierE-ISSN: 1348-4400 ; PMID: 23774861 Version:1
subjectSus Scrofa ; Vitrification ; Cryopreservation -- Instrumentation ; Ovary -- Cytology
descriptionEffects of supporting materials during vitrification procedure on the morphologies of preantral follicles of pig ovaries were assessed. Ovarian cortical sections of prepubertal pigs were randomly allocated to 5 groups. The sections were vitrified ultrarapidly with 5 different vitrification devices. The sections were put on 4 fine needles (Cryosupport), on a thin copper plate, or on a carbon graphite sheet or were sandwiched between copper plates or between carbon graphite sheets before cooling. The cooling and warming rates with the graphite sheets were significantly higher than those with the copper plates (P<0.05). A total of 3,064 follicles were analyzed following HE staining after vitrification with 5 different devices. The morphologies follicles vitrified on the Cryosupport or on the graphite sheet were well preserved compared with those vitrified on the copper plate or between copper plates (P<0.01). The morphologies of follicles vitrified between copper plates were mostly damaged...
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titleGood thermally conducting material supports follicle morphologies of porcine ovaries cryopreserved with ultrarapid vitrification
descriptionEffects of supporting materials during vitrification procedure on the morphologies of preantral follicles of pig ovaries were assessed. Ovarian cortical sections of prepubertal pigs were randomly allocated to 5 groups. The sections were vitrified ultrarapidly with 5 different vitrification devices. The sections were put on 4 fine needles (Cryosupport), on a thin copper plate, or on a carbon graphite sheet or were sandwiched between copper plates or between carbon graphite sheets before cooling. The cooling and warming rates with the graphite sheets were significantly higher than those with the copper plates (P<0.05). A total of 3,064 follicles were analyzed following HE staining after vitrification with 5 different devices. The morphologies follicles vitrified on the Cryosupport or on the graphite sheet were well preserved compared with those vitrified on the copper plate or between copper plates (P<0.01). The morphologies of follicles vitrified between copper plates were mostly damaged...
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titleGood thermally conducting material supports follicle morphologies of porcine ovaries cryopreserved with ultrarapid vitrification
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abstractEffects of supporting materials during vitrification procedure on the morphologies of preantral follicles of pig ovaries were assessed. Ovarian cortical sections of prepubertal pigs were randomly allocated to 5 groups. The sections were vitrified ultrarapidly with 5 different vitrification devices. The sections were put on 4 fine needles (Cryosupport), on a thin copper plate, or on a carbon graphite sheet or were sandwiched between copper plates or between carbon graphite sheets before cooling. The cooling and warming rates with the graphite sheets were significantly higher than those with the copper plates (P<0.05). A total of 3,064 follicles were analyzed following HE staining after vitrification with 5 different devices. The morphologies follicles vitrified on the Cryosupport or on the graphite sheet were well preserved compared with those vitrified on the copper plate or between copper plates (P<0.01). The morphologies of follicles vitrified between copper plates were mostly damaged...
pmid23774861
doi10.1262/jrd.2013-012
date2013-10