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Pluripotent stem cells induced from mouse somatic cells by small-molecule compounds

Pluripotent stem cells can be induced from somatic cells, providing an unlimited cell resource, with potential for studying disease and use in regenerative medicine. However, genetic manipulation and technically challenging strategies such as nuclear transfer used in reprogramming limit their clinic... Full description

Journal Title: Science (New York N.Y.), 09 August 2013, Vol.341(6146), pp.651-4
Main Author: Hou, Pingping
Other Authors: Li, Yanqin , Zhang, Xu , Liu, Chun , Guan, Jingyang , Li, Honggang , Zhao, Ting , Ye, Junqing , Yang, Weifeng , Liu, Kang , Ge, Jian , Xu, Jun , Zhang, Qiang , Zhao, Yang , Deng, Hongkui
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: E-ISSN: 1095-9203 ; PMID: 23868920 Version:1 ; DOI: 10.1126/science.1239278
Link: http://pubmed.gov/23868920
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recordid: medline23868920
title: Pluripotent stem cells induced from mouse somatic cells by small-molecule compounds
format: Article
creator:
  • Hou, Pingping
  • Li, Yanqin
  • Zhang, Xu
  • Liu, Chun
  • Guan, Jingyang
  • Li, Honggang
  • Zhao, Ting
  • Ye, Junqing
  • Yang, Weifeng
  • Liu, Kang
  • Ge, Jian
  • Xu, Jun
  • Zhang, Qiang
  • Zhao, Yang
  • Deng, Hongkui
subjects:
  • Cell Engineering -- Methods
  • Cellular Reprogramming -- Drug Effects
  • Fibroblasts -- Drug Effects
  • Induced Pluripotent Stem Cells -- Cytology
  • Small Molecule Libraries -- Pharmacology
ispartof: Science (New York, N.Y.), 09 August 2013, Vol.341(6146), pp.651-4
description: Pluripotent stem cells can be induced from somatic cells, providing an unlimited cell resource, with potential for studying disease and use in regenerative medicine. However, genetic manipulation and technically challenging strategies such as nuclear transfer used in reprogramming limit their clinical applications. Here, we show that pluripotent stem cells can be generated from mouse somatic cells at a frequency up to 0.2% using a combination of seven small-molecule compounds. The chemically induced pluripotent stem cells resemble embryonic stem cells in terms of their gene expression profiles, epigenetic status, and potential for differentiation and germline transmission. By using small molecules, exogenous "master genes" are dispensable for cell fate reprogramming. This chemical reprogramming strategy has potential use in generating functional desirable cell types for clinical applications.
language: eng
source:
identifier: E-ISSN: 1095-9203 ; PMID: 23868920 Version:1 ; DOI: 10.1126/science.1239278
fulltext: fulltext
issn:
  • 10959203
  • 1095-9203
url: Link


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titlePluripotent stem cells induced from mouse somatic cells by small-molecule compounds
creatorHou, Pingping ; Li, Yanqin ; Zhang, Xu ; Liu, Chun ; Guan, Jingyang ; Li, Honggang ; Zhao, Ting ; Ye, Junqing ; Yang, Weifeng ; Liu, Kang ; Ge, Jian ; Xu, Jun ; Zhang, Qiang ; Zhao, Yang ; Deng, Hongkui
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subjectCell Engineering -- Methods ; Cellular Reprogramming -- Drug Effects ; Fibroblasts -- Drug Effects ; Induced Pluripotent Stem Cells -- Cytology ; Small Molecule Libraries -- Pharmacology
descriptionPluripotent stem cells can be induced from somatic cells, providing an unlimited cell resource, with potential for studying disease and use in regenerative medicine. However, genetic manipulation and technically challenging strategies such as nuclear transfer used in reprogramming limit their clinical applications. Here, we show that pluripotent stem cells can be generated from mouse somatic cells at a frequency up to 0.2% using a combination of seven small-molecule compounds. The chemically induced pluripotent stem cells resemble embryonic stem cells in terms of their gene expression profiles, epigenetic status, and potential for differentiation and germline transmission. By using small molecules, exogenous "master genes" are dispensable for cell fate reprogramming. This chemical reprogramming strategy has potential use in generating functional desirable cell types for clinical applications.
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descriptionPluripotent stem cells can be induced from somatic cells, providing an unlimited cell resource, with potential for studying disease and use in regenerative medicine. However, genetic manipulation and technically challenging strategies such as nuclear transfer used in reprogramming limit their clinical applications. Here, we show that pluripotent stem cells can be generated from mouse somatic cells at a frequency up to 0.2% using a combination of seven small-molecule compounds. The chemically induced pluripotent stem cells resemble embryonic stem cells in terms of their gene expression profiles, epigenetic status, and potential for differentiation and germline transmission. By using small molecules, exogenous "master genes" are dispensable for cell fate reprogramming. This chemical reprogramming strategy has potential use in generating functional desirable cell types for clinical applications.
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authorHou, Pingping ; Li, Yanqin ; Zhang, Xu ; Liu, Chun ; Guan, Jingyang ; Li, Honggang ; Zhao, Ting ; Ye, Junqing ; Yang, Weifeng ; Liu, Kang ; Ge, Jian ; Xu, Jun ; Zhang, Qiang ; Zhao, Yang ; Deng, Hongkui
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abstractPluripotent stem cells can be induced from somatic cells, providing an unlimited cell resource, with potential for studying disease and use in regenerative medicine. However, genetic manipulation and technically challenging strategies such as nuclear transfer used in reprogramming limit their clinical applications. Here, we show that pluripotent stem cells can be generated from mouse somatic cells at a frequency up to 0.2% using a combination of seven small-molecule compounds. The chemically induced pluripotent stem cells resemble embryonic stem cells in terms of their gene expression profiles, epigenetic status, and potential for differentiation and germline transmission. By using small molecules, exogenous "master genes" are dispensable for cell fate reprogramming. This chemical reprogramming strategy has potential use in generating functional desirable cell types for clinical applications.
doi10.1126/science.1239278
pmid23868920
issn00368075
oafree_for_read
date2013-08-09