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Direct detection of unamplified hepatoma upregulated protein RNA in urine using gold nanoparticles for bladder cancer diagnosis

To develop a gold nanoparticle (AuNP) assay for direct detection of unamplified HURP RNA in urine. HURP RNA was extracted from urine samples (50 bladder carcinoma patients, 25 benign bladder lesions, and 25 controls) and further purified using magnetic nanoparticles (MNPs), functionalized with HURP... Full description

Journal Title: Clinical biochemistry January 2014, Vol.47(1-2), pp.104-10
Main Author: Eissa, Sanaa
Other Authors: Shawky, Sherif M , Matboli, Marwa , Mohamed, Shaymaa , Azzazy, Hassan M E
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: E-ISSN: 1873-2933 ; PMID: 24183881 Version:1 ; DOI: 10.1016/j.clinbiochem.2013.10.022
Link: http://pubmed.gov/24183881
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recordid: medline24183881
title: Direct detection of unamplified hepatoma upregulated protein RNA in urine using gold nanoparticles for bladder cancer diagnosis
format: Article
creator:
  • Eissa, Sanaa
  • Shawky, Sherif M
  • Matboli, Marwa
  • Mohamed, Shaymaa
  • Azzazy, Hassan M E
subjects:
  • Bladder Carcinoma
  • Gold Nanoparticles
  • Hepatoma Upregulated Protein
  • Magnetic Nanoparticles
  • Squamous Cell Carcinoma
  • Transitional Cell Carcinoma
  • Urinary Biomarkers
  • Metal Nanoparticles
  • Gold -- Chemistry
  • Neoplasm Proteins -- Genetics
  • RNA -- Urine
  • Urinary Bladder Neoplasms -- Diagnosis
ispartof: Clinical biochemistry, January 2014, Vol.47(1-2), pp.104-10
description: To develop a gold nanoparticle (AuNP) assay for direct detection of unamplified HURP RNA in urine. HURP RNA was extracted from urine samples (50 bladder carcinoma patients, 25 benign bladder lesions, and 25 controls) and further purified using magnetic nanoparticles (MNPs), functionalized with HURP RNA-specific oligonucleotides, and then detected by RT-PCR or gold nanoparticles. The developed HURP RNA AuNP assay has a sensitivity and a specificity of 88.5% and 94%, respectively, and a detection limit of 2.4 nmol/L. The concordance between the HURP AuNP assay with RT-PCR after RNA purification using functionalized MNPs was 97%. The developed colorimetric HURP RNA AuNP assay is sensitive, simple, and can aid noninvasive diagnosis of bladder cancer.
language: eng
source:
identifier: E-ISSN: 1873-2933 ; PMID: 24183881 Version:1 ; DOI: 10.1016/j.clinbiochem.2013.10.022
fulltext: fulltext
issn:
  • 18732933
  • 1873-2933
url: Link


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titleDirect detection of unamplified hepatoma upregulated protein RNA in urine using gold nanoparticles for bladder cancer diagnosis
creatorEissa, Sanaa ; Shawky, Sherif M ; Matboli, Marwa ; Mohamed, Shaymaa ; Azzazy, Hassan M E
ispartofClinical biochemistry, January 2014, Vol.47(1-2), pp.104-10
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subjectBladder Carcinoma ; Gold Nanoparticles ; Hepatoma Upregulated Protein ; Magnetic Nanoparticles ; Squamous Cell Carcinoma ; Transitional Cell Carcinoma ; Urinary Biomarkers ; Metal Nanoparticles ; Gold -- Chemistry ; Neoplasm Proteins -- Genetics ; RNA -- Urine ; Urinary Bladder Neoplasms -- Diagnosis
descriptionTo develop a gold nanoparticle (AuNP) assay for direct detection of unamplified HURP RNA in urine. HURP RNA was extracted from urine samples (50 bladder carcinoma patients, 25 benign bladder lesions, and 25 controls) and further purified using magnetic nanoparticles (MNPs), functionalized with HURP RNA-specific oligonucleotides, and then detected by RT-PCR or gold nanoparticles. The developed HURP RNA AuNP assay has a sensitivity and a specificity of 88.5% and 94%, respectively, and a detection limit of 2.4 nmol/L. The concordance between the HURP AuNP assay with RT-PCR after RNA purification using functionalized MNPs was 97%. The developed colorimetric HURP RNA AuNP assay is sensitive, simple, and can aid noninvasive diagnosis of bladder cancer.
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2The developed HURP RNA AuNP assay has a sensitivity and a specificity of 88.5% and 94%, respectively, and a detection limit of 2.4 nmol/L. The concordance between the HURP AuNP assay with RT-PCR after RNA purification using functionalized MNPs was 97%.
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titleDirect detection of unamplified hepatoma upregulated protein RNA in urine using gold nanoparticles for bladder cancer diagnosis
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abstractTo develop a gold nanoparticle (AuNP) assay for direct detection of unamplified HURP RNA in urine.
doi10.1016/j.clinbiochem.2013.10.022
pmid24183881
date2014-01