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A comparative study on the effects of pristine and functionalized single-walled carbon nanotubes on osteoblasts: ultrastructural and biochemical properties

A comparative study was performed to investigate the ultrastructural and biomolecular properties of osteoblasts induced by three types of single-walled carbon nanotubes (SWNTs). The results on cellular uptake and ultrastructural alteration indicate that SWNTs enter osteoblasts by endocytosis. SWNTs-... Full description

Journal Title: Journal of materials science. Materials in medicine August 2014, Vol.25(8), pp.1915-23
Main Author: Tong, Qiu
Other Authors: Qingzhi, Wu , Honglian, Dai , Xinyu, Wang , Youfa, Wang , Shipu, Li , Junli, Li
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: E-ISSN: 1573-4838 ; PMID: 24875669 Version:1 ; DOI: 10.1007/s10856-014-5227-z
Link: http://pubmed.gov/24875669
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recordid: medline24875669
title: A comparative study on the effects of pristine and functionalized single-walled carbon nanotubes on osteoblasts: ultrastructural and biochemical properties
format: Article
creator:
  • Tong, Qiu
  • Qingzhi, Wu
  • Honglian, Dai
  • Xinyu, Wang
  • Youfa, Wang
  • Shipu, Li
  • Junli, Li
subjects:
  • Nanotubes, Carbon
  • Osteoblasts -- Drug Effects
ispartof: Journal of materials science. Materials in medicine, August 2014, Vol.25(8), pp.1915-23
description: A comparative study was performed to investigate the ultrastructural and biomolecular properties of osteoblasts induced by three types of single-walled carbon nanotubes (SWNTs). The results on cellular uptake and ultrastructural alteration indicate that SWNTs enter osteoblasts by endocytosis. SWNTs-COOH and SWNTs-OH particles were freely dispersed in the cytoplasm, while pristine SWNTs were localized to the periphery of the cell. Both SWNTs-OH and SWNTs-COOH promoted cell changes in cell activity regarding mRNA expression at doses of 50 and 100 μg/mL in the first 24 h. When treated with 50 μg/mL SWNTs-COOH for 48 h, the expression of type I collagen increased by 6.3-fold (for MG63) or 9.1-fold (for primary osteoblasts) compared with the control group. The present study observed for the first time that SWNTs-COOH initiated the prompt and the maximum upregulation of type I collagen gene expression, and simultaneously induced the expansion of the endoplasmic reticulum for increased protein synthesis, which in turn accelerated the mineralization process. However, impaired cell properties and mitochondrial injury were detected following treatment with SWNTs at 100 μg/mL after 48 h. In conclusion, we believe that SWNTs-COOH is a good candidate for the fabrication of biomedical scaffolds for bone regeneration.
language: eng
source:
identifier: E-ISSN: 1573-4838 ; PMID: 24875669 Version:1 ; DOI: 10.1007/s10856-014-5227-z
fulltext: fulltext
issn:
  • 15734838
  • 1573-4838
url: Link


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titleA comparative study on the effects of pristine and functionalized single-walled carbon nanotubes on osteoblasts: ultrastructural and biochemical properties
creatorTong, Qiu ; Qingzhi, Wu ; Honglian, Dai ; Xinyu, Wang ; Youfa, Wang ; Shipu, Li ; Junli, Li
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descriptionA comparative study was performed to investigate the ultrastructural and biomolecular properties of osteoblasts induced by three types of single-walled carbon nanotubes (SWNTs). The results on cellular uptake and ultrastructural alteration indicate that SWNTs enter osteoblasts by endocytosis. SWNTs-COOH and SWNTs-OH particles were freely dispersed in the cytoplasm, while pristine SWNTs were localized to the periphery of the cell. Both SWNTs-OH and SWNTs-COOH promoted cell changes in cell activity regarding mRNA expression at doses of 50 and 100 μg/mL in the first 24 h. When treated with 50 μg/mL SWNTs-COOH for 48 h, the expression of type I collagen increased by 6.3-fold (for MG63) or 9.1-fold (for primary osteoblasts) compared with the control group. The present study observed for the first time that SWNTs-COOH initiated the prompt and the maximum upregulation of type I collagen gene expression, and simultaneously induced the expansion of the endoplasmic reticulum for increased protein synthesis, which in turn accelerated the mineralization process. However, impaired cell properties and mitochondrial injury were detected following treatment with SWNTs at 100 μg/mL after 48 h. In conclusion, we believe that SWNTs-COOH is a good candidate for the fabrication of biomedical scaffolds for bone regeneration.
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abstractA comparative study was performed to investigate the ultrastructural and biomolecular properties of osteoblasts induced by three types of single-walled carbon nanotubes (SWNTs). The results on cellular uptake and ultrastructural alteration indicate that SWNTs enter osteoblasts by endocytosis. SWNTs-COOH and SWNTs-OH particles were freely dispersed in the cytoplasm, while pristine SWNTs were localized to the periphery of the cell. Both SWNTs-OH and SWNTs-COOH promoted cell changes in cell activity regarding mRNA expression at doses of 50 and 100 μg/mL in the first 24 h. When treated with 50 μg/mL SWNTs-COOH for 48 h, the expression of type I collagen increased by 6.3-fold (for MG63) or 9.1-fold (for primary osteoblasts) compared with the control group. The present study observed for the first time that SWNTs-COOH initiated the prompt and the maximum upregulation of type I collagen gene expression, and simultaneously induced the expansion of the endoplasmic reticulum for increased protein synthesis, which in turn accelerated the mineralization process. However, impaired cell properties and mitochondrial injury were detected following treatment with SWNTs at 100 μg/mL after 48 h. In conclusion, we believe that SWNTs-COOH is a good candidate for the fabrication of biomedical scaffolds for bone regeneration.
doi10.1007/s10856-014-5227-z
pmid24875669
issn09574530
oafree_for_read
date2014-08