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'Hit and run' serial femtosecond crystallography of a membrane kinase in the lipid cubic phase

The lipid-based bicontinuous cubic mesophase is a nanoporous membrane mimetic with applications in areas that include medicine, personal care products, foods and the basic sciences. An application of particular note concerns it use as a medium in which to grow crystals of membrane proteins for struc... Full description

Journal Title: Philosophical transactions of the Royal Society of London. Series B Biological sciences, 17 July 2014, Vol.369(1647), pp.20130621
Main Author: Caffrey, Martin
Other Authors: Li, Dianfan , Howe, Nicole , Shah, Syed T A
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: E-ISSN: 1471-2970 ; PMID: 24914170 Version:1 ; DOI: 10.1098/rstb.2013.0621
Link: http://pubmed.gov/24914170
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recordid: medline24914170
title: 'Hit and run' serial femtosecond crystallography of a membrane kinase in the lipid cubic phase
format: Article
creator:
  • Caffrey, Martin
  • Li, Dianfan
  • Howe, Nicole
  • Shah, Syed T A
subjects:
  • X-Ray Free-Electron Laser
  • Crystal Structure
  • Enzyme
  • Membrane Protein
  • Mesophase
  • Monoacylglycerol
  • Electrons
  • Lasers
  • Crystallography, X-Ray -- Methods
  • Diacylglycerol Kinase -- Chemistry
  • X-Ray Diffraction -- Methods
ispartof: Philosophical transactions of the Royal Society of London. Series B, Biological sciences, 17 July 2014, Vol.369(1647), pp.20130621
description: The lipid-based bicontinuous cubic mesophase is a nanoporous membrane mimetic with applications in areas that include medicine, personal care products, foods and the basic sciences. An application of particular note concerns it use as a medium in which to grow crystals of membrane proteins for structure determination by X-ray crystallography. At least two variations of the mesophase exist. One is the highly viscous cubic phase, which has well developed long-range order. The other so-called sponge phase is considerably more fluid and lacks long-range order. The sponge phase has recently been shown to be a convenient vehicle for delivering microcrystals of membrane proteins to an X-ray free-electron laser beam for serial femtosecond crystallography (SFX). Unfortunately, the sponge phase approach calls for large amounts of protein that are not always available in the case of membrane proteins. The cubic phase offers the advantage of requiring significantly less protein for SFX but comes with its own challenges. Here, we describe the physico-chemical bases for these challenges, solutions to them and prospects for future uses of lipidic mesophases in the SFX arena.
language: eng
source:
identifier: E-ISSN: 1471-2970 ; PMID: 24914170 Version:1 ; DOI: 10.1098/rstb.2013.0621
fulltext: fulltext
issn:
  • 14712970
  • 1471-2970
url: Link


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title'Hit and run' serial femtosecond crystallography of a membrane kinase in the lipid cubic phase
creatorCaffrey, Martin ; Li, Dianfan ; Howe, Nicole ; Shah, Syed T A
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subjectX-Ray Free-Electron Laser ; Crystal Structure ; Enzyme ; Membrane Protein ; Mesophase ; Monoacylglycerol ; Electrons ; Lasers ; Crystallography, X-Ray -- Methods ; Diacylglycerol Kinase -- Chemistry ; X-Ray Diffraction -- Methods
descriptionThe lipid-based bicontinuous cubic mesophase is a nanoporous membrane mimetic with applications in areas that include medicine, personal care products, foods and the basic sciences. An application of particular note concerns it use as a medium in which to grow crystals of membrane proteins for structure determination by X-ray crystallography. At least two variations of the mesophase exist. One is the highly viscous cubic phase, which has well developed long-range order. The other so-called sponge phase is considerably more fluid and lacks long-range order. The sponge phase has recently been shown to be a convenient vehicle for delivering microcrystals of membrane proteins to an X-ray free-electron laser beam for serial femtosecond crystallography (SFX). Unfortunately, the sponge phase approach calls for large amounts of protein that are not always available in the case of membrane proteins. The cubic phase offers the advantage of requiring significantly less protein for SFX but comes with its own challenges. Here, we describe the physico-chemical bases for these challenges, solutions to them and prospects for future uses of lipidic mesophases in the SFX arena.
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abstractThe lipid-based bicontinuous cubic mesophase is a nanoporous membrane mimetic with applications in areas that include medicine, personal care products, foods and the basic sciences. An application of particular note concerns it use as a medium in which to grow crystals of membrane proteins for structure determination by X-ray crystallography. At least two variations of the mesophase exist. One is the highly viscous cubic phase, which has well developed long-range order. The other so-called sponge phase is considerably more fluid and lacks long-range order. The sponge phase has recently been shown to be a convenient vehicle for delivering microcrystals of membrane proteins to an X-ray free-electron laser beam for serial femtosecond crystallography (SFX). Unfortunately, the sponge phase approach calls for large amounts of protein that are not always available in the case of membrane proteins. The cubic phase offers the advantage of requiring significantly less protein for SFX but comes with its own challenges. Here, we describe the physico-chemical bases for these challenges, solutions to them and prospects for future uses of lipidic mesophases in the SFX arena.
doi10.1098/rstb.2013.0621
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date2014-07-17