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The regulatory effect of UL-16 binding protein-3 expression on the cytotoxicity of NK cells in cancer patients

The activating immunoreceptor NKG2D (natural killer group 2, member D) and its ligands play important roles in the innate and adaptive immune responses. UL16-binding protein 3 (ULBP3), an NKG2D ligand, is overexpressed on certain epithelial tumor cells. In this study, we investigated the effect of U... Full description

Journal Title: Scientific reports 20 August 2014, Vol.4, pp.6138
Main Author: Mou, Xiao
Other Authors: Zhou, Yuepeng , Jiang, Peng , Zhou, Tong , Jiang, Qian , Xu, Chengcheng , Liu, Hongli , Zheng, Tingting , Yuan, Guoyue , Zhang, Yanyun , Chen, Deyu , Mao, Chaoming
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: E-ISSN: 2045-2322 ; PMID: 25138242 Version:1 ; DOI: 10.1038/srep06138
Link: http://pubmed.gov/25138242
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recordid: medline25138242
title: The regulatory effect of UL-16 binding protein-3 expression on the cytotoxicity of NK cells in cancer patients
format: Article
creator:
  • Mou, Xiao
  • Zhou, Yuepeng
  • Jiang, Peng
  • Zhou, Tong
  • Jiang, Qian
  • Xu, Chengcheng
  • Liu, Hongli
  • Zheng, Tingting
  • Yuan, Guoyue
  • Zhang, Yanyun
  • Chen, Deyu
  • Mao, Chaoming
subjects:
  • Intercellular Signaling Peptides and Proteins -- Metabolism
  • Killer Cells, Natural -- Immunology
  • Neoplasms -- Pathology
ispartof: Scientific reports, 20 August 2014, Vol.4, pp.6138
description: The activating immunoreceptor NKG2D (natural killer group 2, member D) and its ligands play important roles in the innate and adaptive immune responses. UL16-binding protein 3 (ULBP3), an NKG2D ligand, is overexpressed on certain epithelial tumor cells. In this study, we investigated the effect of ULBP3 expression on the cytotoxic activity of natural killer (NK) cells. ULBP3 were measured by flow cytometry analysis, immunohistochemistry, and time-resolved fluoroimmunoassay. The cytotoxicity of NK cells was determined with the lactate dehydrogenase release assay. We found that ULBP3 was overexpressed on tumor cell lines and tumor tissues. Serum from cancer patients, but not from healthy donors, contained elevated levels of soluble ULBP3 (sULBP3). Importantly, high expression of ULBP3 on the cell surface of tumor cells augmented NKG2D-mediated NK cell cytotoxicity. However, low levels of sULBP3 (
language: eng
source:
identifier: E-ISSN: 2045-2322 ; PMID: 25138242 Version:1 ; DOI: 10.1038/srep06138
fulltext: fulltext
issn:
  • 20452322
  • 2045-2322
url: Link


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titleThe regulatory effect of UL-16 binding protein-3 expression on the cytotoxicity of NK cells in cancer patients
creatorMou, Xiao ; Zhou, Yuepeng ; Jiang, Peng ; Zhou, Tong ; Jiang, Qian ; Xu, Chengcheng ; Liu, Hongli ; Zheng, Tingting ; Yuan, Guoyue ; Zhang, Yanyun ; Chen, Deyu ; Mao, Chaoming
ispartofScientific reports, 20 August 2014, Vol.4, pp.6138
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subjectIntercellular Signaling Peptides and Proteins -- Metabolism ; Killer Cells, Natural -- Immunology ; Neoplasms -- Pathology
descriptionThe activating immunoreceptor NKG2D (natural killer group 2, member D) and its ligands play important roles in the innate and adaptive immune responses. UL16-binding protein 3 (ULBP3), an NKG2D ligand, is overexpressed on certain epithelial tumor cells. In this study, we investigated the effect of ULBP3 expression on the cytotoxic activity of natural killer (NK) cells. ULBP3 were measured by flow cytometry analysis, immunohistochemistry, and time-resolved fluoroimmunoassay. The cytotoxicity of NK cells was determined with the lactate dehydrogenase release assay. We found that ULBP3 was overexpressed on tumor cell lines and tumor tissues. Serum from cancer patients, but not from healthy donors, contained elevated levels of soluble ULBP3 (sULBP3). Importantly, high expression of ULBP3 on the cell surface of tumor cells augmented NKG2D-mediated NK cell cytotoxicity. However, low levels of sULBP3 (<15 ng/ml) weakened the cytotoxicity of NK cells by decreasing NKG2D expression on NK cells. Further...
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descriptionThe activating immunoreceptor NKG2D (natural killer group 2, member D) and its ligands play important roles in the innate and adaptive immune responses. UL16-binding protein 3 (ULBP3), an NKG2D ligand, is overexpressed on certain epithelial tumor cells. In this study, we investigated the effect of ULBP3 expression on the cytotoxic activity of natural killer (NK) cells. ULBP3 were measured by flow cytometry analysis, immunohistochemistry, and time-resolved fluoroimmunoassay. The cytotoxicity of NK cells was determined with the lactate dehydrogenase release assay. We found that ULBP3 was overexpressed on tumor cell lines and tumor tissues. Serum from cancer patients, but not from healthy donors, contained elevated levels of soluble ULBP3 (sULBP3). Importantly, high expression of ULBP3 on the cell surface of tumor cells augmented NKG2D-mediated NK cell cytotoxicity. However, low levels of sULBP3 (<15 ng/ml) weakened the cytotoxicity of NK cells by decreasing NKG2D expression on NK cells. Further...
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abstractThe activating immunoreceptor NKG2D (natural killer group 2, member D) and its ligands play important roles in the innate and adaptive immune responses. UL16-binding protein 3 (ULBP3), an NKG2D ligand, is overexpressed on certain epithelial tumor cells. In this study, we investigated the effect of ULBP3 expression on the cytotoxic activity of natural killer (NK) cells. ULBP3 were measured by flow cytometry analysis, immunohistochemistry, and time-resolved fluoroimmunoassay. The cytotoxicity of NK cells was determined with the lactate dehydrogenase release assay. We found that ULBP3 was overexpressed on tumor cell lines and tumor tissues. Serum from cancer patients, but not from healthy donors, contained elevated levels of soluble ULBP3 (sULBP3). Importantly, high expression of ULBP3 on the cell surface of tumor cells augmented NKG2D-mediated NK cell cytotoxicity. However, low levels of sULBP3 (<15 ng/ml) weakened the cytotoxicity of NK cells by decreasing NKG2D expression on NK cells. Further...
doi10.1038/srep06138
pmid25138242
issue1
date2014-08-20