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Optical imaging. Expansion microscopy

In optical microscopy, fine structural details are resolved by using refraction to magnify images of a specimen. We discovered that by synthesizing a swellable polymer network within a specimen, it can be physically expanded, resulting in physical magnification. By covalently anchoring specific labe... Full description

Journal Title: Science (New York N.Y.), 30 January 2015, Vol.347(6221), pp.543-8
Main Author: Chen, Fei
Other Authors: Tillberg, Paul W , Boyden, Edward S
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: E-ISSN: 1095-9203 ; PMID: 25592419 Version:1 ; DOI: 10.1126/science.1260088
Link: http://pubmed.gov/25592419
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recordid: medline25592419
title: Optical imaging. Expansion microscopy
format: Article
creator:
  • Chen, Fei
  • Tillberg, Paul W
  • Boyden, Edward S
subjects:
  • Coated Pits, Cell-Membrane -- Ultrastructure
  • Hippocampus -- Ultrastructure
  • Microscopy -- Methods
  • Microtubules -- Ultrastructure
  • Optical Imaging -- Methods
ispartof: Science (New York, N.Y.), 30 January 2015, Vol.347(6221), pp.543-8
description: In optical microscopy, fine structural details are resolved by using refraction to magnify images of a specimen. We discovered that by synthesizing a swellable polymer network within a specimen, it can be physically expanded, resulting in physical magnification. By covalently anchoring specific labels located within the specimen directly to the polymer network, labels spaced closer than the optical diffraction limit can be isotropically separated and optically resolved, a process we call expansion microscopy (ExM). Thus, this process can be used to perform scalable superresolution microscopy with diffraction-limited microscopes. We demonstrate ExM with apparent ~70-nanometer lateral resolution in both cultured cells and brain tissue, performing three-color superresolution imaging of ~10(7) cubic micrometers of the mouse hippocampus with a conventional confocal microscope.
language: eng
source:
identifier: E-ISSN: 1095-9203 ; PMID: 25592419 Version:1 ; DOI: 10.1126/science.1260088
fulltext: no_fulltext
issn:
  • 10959203
  • 1095-9203
url: Link


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subjectCoated Pits, Cell-Membrane -- Ultrastructure ; Hippocampus -- Ultrastructure ; Microscopy -- Methods ; Microtubules -- Ultrastructure ; Optical Imaging -- Methods
descriptionIn optical microscopy, fine structural details are resolved by using refraction to magnify images of a specimen. We discovered that by synthesizing a swellable polymer network within a specimen, it can be physically expanded, resulting in physical magnification. By covalently anchoring specific labels located within the specimen directly to the polymer network, labels spaced closer than the optical diffraction limit can be isotropically separated and optically resolved, a process we call expansion microscopy (ExM). Thus, this process can be used to perform scalable superresolution microscopy with diffraction-limited microscopes. We demonstrate ExM with apparent ~70-nanometer lateral resolution in both cultured cells and brain tissue, performing three-color superresolution imaging of ~10(7) cubic micrometers of the mouse hippocampus with a conventional confocal microscope.
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descriptionIn optical microscopy, fine structural details are resolved by using refraction to magnify images of a specimen. We discovered that by synthesizing a swellable polymer network within a specimen, it can be physically expanded, resulting in physical magnification. By covalently anchoring specific labels located within the specimen directly to the polymer network, labels spaced closer than the optical diffraction limit can be isotropically separated and optically resolved, a process we call expansion microscopy (ExM). Thus, this process can be used to perform scalable superresolution microscopy with diffraction-limited microscopes. We demonstrate ExM with apparent ~70-nanometer lateral resolution in both cultured cells and brain tissue, performing three-color superresolution imaging of ~10(7) cubic micrometers of the mouse hippocampus with a conventional confocal microscope.
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abstractIn optical microscopy, fine structural details are resolved by using refraction to magnify images of a specimen. We discovered that by synthesizing a swellable polymer network within a specimen, it can be physically expanded, resulting in physical magnification. By covalently anchoring specific labels located within the specimen directly to the polymer network, labels spaced closer than the optical diffraction limit can be isotropically separated and optically resolved, a process we call expansion microscopy (ExM). Thus, this process can be used to perform scalable superresolution microscopy with diffraction-limited microscopes. We demonstrate ExM with apparent ~70-nanometer lateral resolution in both cultured cells and brain tissue, performing three-color superresolution imaging of ~10(7) cubic micrometers of the mouse hippocampus with a conventional confocal microscope.
doi10.1126/science.1260088
pmid25592419
date2015-01-30